中国组织工程研究

中国组织工程研究 ›› 2024, Vol. 28 ›› Issue (16): 2605-2612.doi: 10.12307/2024.281

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

低频脉冲磁场诱导TRPC1改善COVID-19患者康复期下肢的肌肉无力症状

厉中山1，2，包义君3，刘  洁4，孔维签5，李  伟5，陈  琳6，白  石5，7，杨铁黎8，王春露9   

  1. 1福建师范大学体育科学学院，福建省福州市  350117；2东北大学体育部，辽宁省沈阳市  110819；3中国医科大学附属第四医院，辽宁省沈阳市110044；4中国医科大学科学试验研究中心，辽宁省沈阳市  110122；5沈阳工业大学信息科学与工程学院，辽宁省沈阳市  111003；6东北大学生命科学与健康学院，辽宁省沈阳市  110167；7辽宁省磁医学检测与治疗专业技术创新中心，辽宁省沈阳市  110034；8首都体育学院，北京市100191；9北京体育大学冰上运动学院，北京市  100084
  • 收稿日期:2023-03-15 接受日期:2023-04-01 出版日期:2024-06-08 发布日期:2023-07-31
  • 通讯作者: 白石，沈阳工业大学信息科学与工程学院，辽宁省沈阳市 111003；辽宁省磁医学检测与治疗专业技术创新中心，辽宁省沈阳市 110034 杨铁黎，首都体育学院，北京市 100191 王春露，北京体育大学冰上运动学院，北京市 100084
  • 作者简介:厉中山，体育教育训练学在读博士。
  • 基金资助:
    国家自然科学基金青年项目(62001313)，项目负责人：白石；中国大学生体育协会一般项目(L202103003)，项目负责人：厉中山

Low-frequency pulsed magnetic field induces classical transient receptor potential channels 1 to relieve lower limb muscle weakness in patients recovering from COVID-19

Li Zhongshan1, 2, Bao Yijun3, Liu Jie4, Kong Weiqian5, Li Wei5, Chen Lin6, Bai Shi5, 7, Yang Tieli8, Wang Chunlu9   

  1. 1College of Sports Science, Fujian Normal University, Fuzhou 350117, Fujian Province, China; 2Department of Physical Education, Northeastern University, Shenyang 110819, Liaoning Province, China; 3The Fourth Affiliated Hospital of China Medical University, Shenyang 110044, Liaoning Province, China; 4Experimental Research Center, China Medical University, Shenyang 110122, Liaoning Province, China; 5College of Information Science and Engineering, Shenyang University of Technology, Shenyang 111003, Liaoning Province, China; 6College of Life Science and Health, Northeastern University, Shenyang 110167, Liaoning Province, China; 7Magnetic Medical Detection and Treatment Innovation Center of Liaoning Province, Shenyang 110034, Liaoning Province, China; 8Capital University of Physical Education and Sports, Beijing 100191, China; 9College of Ice Sports, Beijing Sport University, Beijing 100084, China
  • Received:2023-03-15 Accepted:2023-04-01 Online:2024-06-08 Published:2023-07-31
  • Contact: Bai Shi, College of Information Science and Engineering, Shenyang University of Technology, Shenyang 111003, Liaoning Province, China; Magnetic Medical Detection and Treatment Innovation Center of Liaoning Province, Shenyang 110034, Liaoning Province, China Yang Tieli, Capital University of Physical Education and Sports, Beijing 100191, China Wang Chunlu, College of Ice Sports, Beijing Sport University, Beijing 100084, China
  • About author:Li Zhongshan, PhD candidate, College of Sports Science, Fujian Normal University, Fuzhou 350117, Fujian Province, China; Department of Physical Education, Northeastern University, Shenyang 110819, Liaoning Province, China
  • Supported by:
    the National Natural Science Foundation of China (Youth Program), No. 62001313 (to BS); General Project of Federation of University Sports of China, No. L202103003 (to LZS)

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摘要：


文题释义：

新型冠状病毒(COVID-19)：是具有流感症状，并易导致肺部感染的病毒。与其他病毒相似，人体感染新型冠状病毒(COVID-19)后会触发多种免疫反应，对肌肉组织产生酸痛与能量供应限制的影响。
瞬时感受器电位通道1：是一类定位在质膜上的非选择性阳离子通道，可通过改变膜电位或细胞内 Ca2+浓度来充当信号转换器。


背景：肌肉无力是新型冠状病毒(COVID-19)感染后的常见症状，影响康复期人体日常活动能力。在强度1.5 mT，频率3 300 Hz的低频脉冲磁场刺激下可通过诱导和激活经典瞬时感受器电位通1(classical transient receptor potential channel 1，TRPC1)，提升人体骨骼肌的最大自主收缩力与力量耐力，对肌肉组织产生一系列生理支持效应，该手段是否会改善新型冠状病毒肺炎患者康复期的肌无力症状尚无研究。

目的：选用低频脉冲磁场对新型冠状病毒肺炎患者下肢肌群进行磁刺激，以观察该刺激对新型冠状病毒肺炎患者康复期下肢肌群肌无力改善的影响。  
方法：招募胶体金法抗原检测试剂(COVID-19)为阳性并伴有肌肉无力症状的新型冠状病毒(奥密克戎毒株)感染患者14例，将所有受试者随机分成2组，分别为接受磁场刺激的试验组和接受假治疗的对照组。试验总时长3周，试验组每隔48 h对腿部进行低频脉冲磁刺激，对照组与试验组干预流程一致但给予假刺激，两组患者均不被告知磁刺激仪器是否运行，两组患者共进行9次操作，随后观察两组患者下肢局部肌群最大自主收缩力、腿部爆发力与力量耐力的变化情况。

结果与结论：①在采集的8个局部肌群中，试验组患者7个局部肌群在经过3周的低频脉冲磁场刺激，最大自主收缩力值均增长。对照组除3个肌群最大自主收缩力自行增长改善以外，其他肌群肌力无提升。②试验组的左腿前群与双腿后群提升率显著高于对照组。③两组的纵跳摸高高度与膝关节峰值角速度相比试验前测均提升，试验组摸高高度提升率高于对照组。④在疲劳状态下，试验组膝关节峰值角速度下降率显著下降，对照组膝关节峰值角速度下降率无显著性变化；试验组摸高高度下降率显著下降，而对照组摸高高度下降率无显著性变化。⑤上述数据证实，在强度1.5 mT，频率3 300 Hz的低频脉冲磁场刺激方案下，新型冠状病毒肺炎患者在康复期经过3周的低频脉冲磁场刺激相比人体自愈过程可使更多的下肢局部肌群肌力获得提升，对基于腿部爆发力的全身协调发力能力及功能状态明显改善。因此，低频脉冲磁场刺激可作为一种改善新冠感染患者下肢肌肉无力症状的有效、非运动的康复手段。

https://orcid.org/0000-0002-9010-609X(厉中山)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 新型冠状病毒, COVID-19, 新型冠状病毒肺炎, 脉冲磁场, 经典瞬时感受器电位通道1, TRPC1, 肌肉无力

Abstract: BACKGROUND: Muscle weakness is a common symptom after coronavirus disease 2019 (COVID-19) infection and affects the ability to perform daily activities in humans during recovery. Low-frequency pulsed magnetic field stimulation at a strength of 1.5 mT and a frequency of 3 300 Hz can enhance the maximal voluntary contraction and strength endurance of human skeletal muscle by inducing and activating classical transient receptor potential channel 1 (TRPC1), which produces a series of pathological support effects on muscle tissue. It has not been studied whether this means will improve muscle weakness in patients recovering from COVID-19.
OBJECTIVE: To select the low-frequency pulsed magnetic field for magnetic stimulation of lower limb muscle groups in patients with COVID-19, in order to observe the effect of this stimulation on the improvement of muscle weakness of lower limb muscle groups in patients with COVID-19 during the recovery period.
METHODS: Fourteen patients infected with COVID-19 (Omicron strain) positive for Innovita COVID-19 Ab Test (Colloidal Gold) and accompanied by muscle weakness were recruited and randomly divided into two groups: a test group receiving magnetic field stimulation and a control group receiving sham treatment, respectively. The total duration of the trial was 3 weeks. The test group was given low-frequency pulsed magnetic stimulation of the lower limbs every 48 hours and the control group was given the same intervention procedure as the test group but with sham stimulation. Patients in both groups were not informed whether the magnetic stimulation apparatus was running or not. Nine sessions were performed in both groups and the changes in the maximum voluntary contraction, explosive leg force and strength endurance of the local muscle groups of the lower limbs were subsequently observed in both groups.
RESULTS AND CONCLUSION: Among the eight local muscle groups collected, seven local muscle groups in the test group showed an increase in the maximum voluntary contraction value after 3 weeks of low-frequency pulsed magnetic field stimulation. In the control group, there were only three muscle groups with improvement in the maximum voluntary contraction. The rate of improvement in the anterior and posterior muscle groups of the left leg in the test group was significantly higher than that in the control group. The longitudinal jump height and peak angular velocity of the knee joint in both groups were improved compared with the pre-test measurement, and the elevation rate of jumping height in the test group was higher than that in the control group. Under the fatigue condition, the decline rates of peak angular velocity of the knee joint and jumping height in the test group decreased significantly, while those in the control group did not change significantly. The above data confirmed that the low-frequency pulsed magnetic field stimulation with the intensity of 1.5 mT and frequency of 3 300 Hz could improve the muscle strength of more local muscle groups in the lower limbs of patients with COVID-19 during the recovery period compared with the human self-healing process, and the whole-body coordination ability and functional status based on explosive leg force of the legs could be significantly improved. Therefore, low-frequency pulsed magnetic field stimulation can be used as an effective, non-exercise rehabilitation tool to improve muscle weakness in the lower limbs of patients with COVID-19.

Key words: novel coronavirus, COVID-19, novel coronavirus pneumonia, pulsed magnetic field, classical transient receptor potential channel 1, TRPC1, muscle weakness

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