中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 4998-5001.doi: 10.3969/j.issn.1673-8225.2011.27.013

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

吡咯喹啉醌与神经干细胞的增殖

熊顺华1，李  清2，姚柏春3，赵  丹1   

  1. 湖北医药学院，1生理学教研室， 2附属太和医院麻醉科，3解剖学教研室，湖北省十堰市  442000
  • 收稿日期:2011-01-05 修回日期:2011-04-11 出版日期:2011-07-02 发布日期:2011-07-02
  • 作者简介:熊顺华★，女，1963年生，湖北省武汉市人，2000年武汉大学医学院毕业，硕士，副教授，主要从事神经的生理病理研究。 xiongshunhua@hotmail.com
  • 基金资助:

    郧阳医学院优秀中青年科技创新团队(2005CX01)。

Pyrroloquinoline quinone and proliferation of neural stem cells

Xiong Shun-hua1, Li Qing2, Yao Bai-chun3, Zhao Dan1   

  1. 1Department of Physiology, Hubei University of Medicine, Shiyan  442000, Hubei Province, China
    2Department of Anesthesiology, Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan  442000, Hubei Province, China
    3Department of Anatomy, Hubei University of Medicine, Shiyan  442000, Hubei Province, China
  • Received:2011-01-05 Revised:2011-04-11 Online:2011-07-02 Published:2011-07-02
  • About author:Xiong Shun-hua★, Master, Associate professor, Department of Physiology, Hubei University of Medicine, Shiyan 442000, Hubei Province, China xiongshunhua@hotmail.com
  • Supported by:

    the Science and Technology Innovation Team of Excellent Middle-Aged People and Youth of Yunyang Medical College, No. 2005CX01*

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摘要：

背景：目前国内外学者主要应用生长因子样物质来促进神经干细胞的分裂与增殖，所以作者推测吡咯喹啉醌小分子化合物也能促进神经干细胞的分裂增殖。
目的：体外观察氧化还原酶辅酶吡咯喹啉醌促进鼠成体神经干细胞增殖的作用。
方法：体外培养Wistar胎鼠成体神经干细胞成球体后，于培养液中加入1×10-8 mol/L吡咯喹啉醌，对照组加入同等剂量的Hanks溶液。应用流式细胞仪测细胞周期增殖率，应用免疫组化法和Western Blot测周期蛋白激酶(CDK2)和周期蛋白激酶抑制剂(P27)的表达。
结果与结论：与对照组相比，吡咯喹啉醌处理16 h和24 h后，S期和G2期细胞数的比例显著增高，而细胞的凋亡率降低；CDK2蛋白的表达显著增强，而P27的表达显著降低。结果显示低浓度的吡咯喹啉醌即能促进鼠成体神经干细胞的增殖。

关键词: 吡咯喹啉醌, 神经干细胞, 体外培养, 增殖, 细胞周期蛋白依赖性蛋白激酶, 细胞周期蛋白依赖性激酶抑制剂

Abstract:

BACKGROUND: At present, scholars mainly used growth factor-like substance to promote the division and proliferation of neural stem cells. We assumed that pyrroloquinoline quinone (PQQ) micromolecular compound can accelerate the division and proliferation of neural stem cells.
OBJECTIVE: To investigate the promoting effects of PQQ on the proliferation of rat adult neural stem cells in vitro. 
METHODS: The neural stem cells of Wistar rat were cultured in vitro, and 1×10-8 mol/L PQQ was administered into the culture fluid. An equal volume of Hanks solution was added in the control group. The cell cycle was examined by the flow cytometry. The cyclin-dependent Kinase (CDK2) and cyclin-dependent kinase inhibitor (P27) expressions were detected by immunohistochemical staining and western blot assay.
RESULTS AND CONCLUSION: Compared with the control group, after 16 and 24 hours of PQQ administered into the culture fluid, the rate of cells in S and G2 stage was increased and the apoptosis rate of cells was decreased markedly. The expression of CDK2 protein was noticeably increased, and the expression of P27 protein was decreased. Results suggest that low dose of PQQ could encourage the proliferation of the neural stem cells of rats.

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