中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (25): 4716-4708.doi: 10.3969/j.issn.1673-8225.2010.25.036铸造合金；细胞毒性；生物相容性；细胞因子；口腔生物材料

• 生物材料综述 biomaterial review • 上一篇    下一篇

牙科合金生物相容性评价：细胞培养法的可行与可信？

王莉莉1，刘学政2   

  1. 1辽宁医学院附属第二医院，辽宁省锦州市  121000；2辽宁医学院，辽宁省锦州市  121000
  • 出版日期:2010-06-18 发布日期:2010-06-18
  • 通讯作者: 刘学政，教授，博士生导师，辽宁医学院，辽宁省锦州市 121000
  • 作者简介:王莉莉，女，1979年生，辽宁省朝阳市人，汉族， 2004年辽宁医学院毕业，医师，主要从事口腔修复学临床和教学。 Wanglili511@yahoo.com.cn

Evaluation of oral alloy biocompatibility: Is cell culture method feasible and reliable?

Wang Li-li1, Liu Xue-zheng2   

  1. 1 Second Hospital, Liaoning Medical University, Jinzhou   121000, Liaoning Province, China; 2 Liaoning Medical University, Jinzhou   121000, Liaoning Province, China
  • Online:2010-06-18 Published:2010-06-18
  • Contact: Liu Xue-zheng, Professor, Doctoral supervisor, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China
  • About author:Wang Li-li, Physician, Second Hospital, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China Wanglili511@yahoo.com.cn

PDF

311

摘要：

背景：随着口腔金属材料在修复学领域的大量应用，逐渐暴露出一定的生物毒性。细胞培养法以其操作简便、敏感性高、研究周期短、效费比高等特有的优点成为检测材料毒性的有效途径。
目的：概括体外细胞培养法评价牙科用合金的生物相容性研究状况。
方法：以casting alloy, Biocompatibility, Cytotoxicity,  Cytokine为检索词，检索Pubmed数据库；以铸造合金，生物相容性，细胞毒性，炎症因子为检索词，检索CNKI数据库。纳入牙科用合金对细胞生物活性影响方面的内容。排除牙科用合金的理化特性以及力学方面的研究。
结果与结论：当牙龈组织金属离子释出量超过生理耐受量时，可能导致机体毒性，并改变该区域细胞代谢，出现细胞不同程度的损伤(包括细胞活性，DNA/RNA/蛋白质的合成，胞膜的完整性等)及局部免疫学的变化，严重时可使该区域细胞代谢停止。

关键词: 铸造合金, 细胞毒性, 生物相容性, 细胞因子, 口腔生物材料

Abstract:

BACKGROUND: With the development of oral metal material in prosthodontics, the toxicity of organism is shown. Cell culture method is an effective method to check the toxicity, which is easy, with high degree of sensitivity, short time, high efficiency, and low cost.
OBJECTIVE: To summarize the biocompatibility of oral alloy evaluated by cell culture method.
METHODS: A computer-based online search of Pubmed data base and CNKI was performed for related articles with the key words “casting alloy, biocompatibility, cytotoxicity, cytokine” in English and Chinese. Articles recording the influence of oral metal material on cell bioactivity were included. The research of physical and chemical properties and mechanics of oral alloy was excluded.
RESULT AND CONCLUSION: The quantity of metal ion exceeding tolerance dose which release from gum may lead to toxicity of organism, and change cellular metabolism, ultimately resulting in cell damage in various degrees, such as cytoactivity, DNA/RNA protein synthesis, integrity of cytomembrane, as well as local immunology changed, even stopping cellular metabolism.

中图分类号: