中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (12): 2268-2272.doi: 10.3969/j.issn.1673-8225.2010.12.045

• 药物控释材料 drug delivery materials • 上一篇    下一篇

壳聚糖-抗坏血酸盐胶体用于牙周炎模型大鼠牙周组织的修复与再生：效果验证

王  璇1，贾洪诚2，冯延民3，洪丽华3   

  1. 1首都医科大学附属北京同仁医院口腔科，北京市  100730；2首都医科大学，北京地坛医院口腔科，北京市  100015；3吉林大学口腔医学院，吉林省长春市  130041
  • 出版日期:2010-03-19 发布日期:2010-03-19
  • 通讯作者: 贾洪诚，硕士，主治医师，首都医科大学，北京地坛医院口腔科，北京市 100015
  • 作者简介:王 璇★，女，1976年生，辽宁省沈阳市人，汉族，2003年吉林大学毕业，硕士，主治医师，主要从事牙周病学研究。

Chitosan-ascorbate for periodontal tissue healing and regeneration in a rat periodontitis model
An effectiveness validation

Wang Xuan1, Jia Hong-cheng2, Feng Yan-min3, Hong Li-hua3   

  1. 1 Department of Stomatology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China; 2 Department of Stomatology, Beijing Ditan Hospital, Capital Medical University, Beijing   100015, China; 3 School of Stomatology, Jilin University, Changchun   130041 , Jilin Province, China
  • Online:2010-03-19 Published:2010-03-19
  • Contact: Jia Hong-cheng, Mater, Attending physician, Department of Stomatology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
  • About author:Wang Xuan★, Master, Attending physician, Department of Stomatology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

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摘要：

背景：随着对牙周病的病因和发病机制的深入认识，牙周结缔组织破坏的直接原因在于宿主的免疫反应，而非细菌本身，学者们进行了大量具有宿主调节功能的牙周局部用药的研究。壳聚糖是目前较受关注的一种材料，用其附带抗坏血酸盐来处理牙周疾病值得探索。
目的：评估壳聚糖-抗坏血酸盐促进牙周炎模型大鼠牙周组织修复与再生的作用。
方法：采用56只Wistar大鼠建立牙周炎模型，随机分成两组，实验组用壳聚糖-抗坏血酸盐胶体，对照组用生理盐水，隔天牙周袋底注射治疗。治疗后不同时间进行临床检查、形态学测量和组织学观察。
结果与结论：实验组菌斑指数、牙龈指数、牙周袋深度、附着丧失和牙齿松动度均低于对照组，最大差值分别为1.18(28 d)、0.92  (2 d)、0.33 mm(4 d)、0.49 mm(28 d)、1.69(28 d)。实验组牙槽骨丧失量在0，2，4周分别是(1.18±0.08)，(1.10±0.12), (1.03±0.08) mm；而对照组分别为 (1.17±0.11)，(1.31±0.09)，(1.42±0.14) mm。组织学观察实验组和对照组都没有明显的新附着形成，但实验组炎症浸润程度明显低于对照组，且有再生修复。说明壳聚糖-抗坏血酸盐能够促进牙周炎模型大鼠牙周组织修复与再生，是一种很有治疗意义和开发价值的牙周局部应用药物。

关键词: 壳聚糖, 抗坏血酸盐, 牙周炎, 动物模型, 口腔材料

Abstract:

BACKGROUND: With in-depth understanding of the etiology and pathogenesis of periodontal disease, the direct cause of periodontal connective tissue destruction is the host immune response, rather than the bacteria itself. A large number of studies have been made regarding host response modulators and local application of the periodontal pocket. Chitosan exhibits not only excellent biocompatibility and biodegradability, but also various promising biological activities, such as broad-spectrum antibacterial activity and a variety of host regulatorary effects.
OBJECTIVE: To evaluate the therapeutic effects of chitosan-ascorbate on promoting periodontal tissue healing and regeneration in a rat periodontitis model.
METHODS: Fifty-six Wistar rat models of periodontitis were established and randomly divided into two groups: experimental and control. The experimental group received chitosan-ascorbate injection into the pocket bottom, while the control group received physiological saline injection. At different time points after treatment, clinical examination, morphometric and histopathologic analyses were performed. 
RESULTS AND CONCLUSION: Dental plaque index, gingival index, pocket depth, attachment loss, and tooth mobility were significantly lower in the experimental group than in the control group. The largest difference value was 1.18 (28 days), 0.92 (2 days), 0.33 mm (4 days), 0.49 mm (28 days), 1.69 (28 days), respectively. Alveolar bone lose in the experimental group was (1.18±0.08) mm, (1.10±0.12) mm, and (1.03±0.08) mm after 0, 2, and 4 weeks of treatment, while it was (1.17±0.11) mm, (1.31±0.09) mm, and (1.42±0.14) mm in the control group at the corresponding time points. Histopathologic observation showed that no obvious new attachment formation in the experimental and control groups. Compared to the control group, the inflammatory infiltration was significantly lower in the experimental groups, with the phenomena of regeneration and repair. These findings suggest that chitosan-ascorbate gels can promote periodontal tissue healing and regeneration in a rat periodontitis model and can be used as an adjunct to the nonsurgical periodontal therapy in treatment of periodontitis.

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