中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (27): 5058-5061.doi: 10.3969/j.issn.1673-8225.2010.27.027

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

枸杞多糖对大鼠骨髓间充质干细胞增殖及向内皮谱系分化的影响

林壮琳，卢文岳，陈  萍，郑惠珍   

  1. 广东医学院，广东省湛江市 524023
  • 出版日期:2010-07-02 发布日期:2010-07-02
  • 通讯作者: 郑惠珍，博士，教授，硕士生导师，广东医学院生理学研究室主任，广东省湛江市 524023 zhenghz6@gdmc.edu.cn
  • 作者简介:林壮琳，男，1985年生，广东省汕头市人，汉族，广东医学院在读。
  • 基金资助:

    广东医学院康博创新基金(200801)，湛江市科技计划招标项目(2006-3-3)，广东省自然科学基金(9152402301000028)。

Effects of lycium barbarum polysaccharides on proliferation and differentiation of rat bone marrow mesenchymal stem cells into cells of endothelial lineage  

Lin Zhuang-lin, Lu Wen-yue, Chen Ping, Zheng Hui-zhen   

  1. Guangdong Medical Collehanjiang  524023, Guangdong Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Zheng Hui-zhen, Doctor, Professor, Master’s supervisor, Department of Physiology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China zhenghz6@gdmc.edu.cn
  • About author:Lin Zhuang-lin, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China
  • Supported by:

    the Kangbo Innovation Foundation of Guangdong Medical College, No. 200801*; the Technology Plan Tender Project of Zhanjiang City, No. 2006-3-3*; the Natural Science Foundation of Guangdong Province, No. 9152402301000028*

PDF

707

被引次数

18

摘要：

背景：枸杞多糖可促进生精细胞的增殖，诱导大鼠骨髓间充质干细胞、人脐血间充质干细胞向神经元样细胞分化，枸杞多糖对骨髓间充质干细胞的生物学特性有何影响，有待深入研究。
目的：观察枸杞多糖对大鼠骨髓间充质干细胞增殖及向内皮分化的影响。
方法：采用密度梯度离心法分离SD大鼠骨髓间充质干细胞，MTT法检测细胞增殖率。用HG-DMEN+EGM-2诱导大鼠骨髓间充质干细胞向内皮谱系分化，检测细胞Ⅷ因子相关抗原和荆豆凝集素表达。在培养液中加入或不加入枸杞多糖，分析枸杞多糖对大鼠骨髓间充质干细胞增殖和向内皮谱系分化的影响。
结果与结论：枸杞多糖未改变大鼠骨髓间充质干细胞形态，不影响细胞表面抗原CD29、CD45、CD106的表达，细胞Ⅷ因子相关抗原检测呈阴性反应。但明显提高了大鼠骨髓间充质干细胞的增殖率，延长细胞增殖的高峰时间。大鼠骨髓间充质干细胞用含EGM-2的内皮诱导培养基或内皮诱导培养基+枸杞多糖诱导，未加枸杞多糖诱导组大鼠骨髓间充质干细胞在第10天时已转化为具有内皮特征的细胞；而含有枸杞多糖的内皮诱导液需诱导至第12天，细胞才转化为具有内皮特征的细胞，提示枸杞多糖具有延缓EGM-2诱导的大鼠骨髓间充质干细胞向内皮谱系分化的作用。

关键词: 枸杞多糖, 骨髓间充质干细胞, 增殖, 内皮分化, 大鼠

Abstract:

BACKGROUND: Lycium barbarum polysaccharides (LBP) can promote spermatogenic cell proliferation and induce the differentiation of rat bone marrow messenchymal stem cells (BMSCs) and human umbilical cord blood messenchymal stem cells into neuron-like cells. But, it is unknown that the effects of LBP on biological characteristics of BMSCs, which deserves further study.
OBJECTIVE: To investigate the effects of LBP on proliferation and endothelial differentiation of rat BMSCs.
METHODS: BMSCs were separated from Sprague Dawley rats by gradient centrifugation. Cell proliferation rate was analyzed by MTT assay. Rat BMSCs were induced to differentiate into endothelial lineage cells by HG-DMEN plus EGM-2. These cells were identified as endothelial cells with positive factor Ⅷ and Ulex europaeus agglutinin expression. The effects of LBP on proliferation and differentiation of rat BMSCs into endothelial lineage cells were analyzed by adding LBP or not in the medium.
RESULTS AND CONCLUSION: LBP in medium did not change the cellular morphology and did not affect the expression of cell surface marker CD29, CD45 or CD106 and with negative factor Ⅷ. However, LBP speeded up the proliferation of rat BMSCs and delayed its peak proliferation time. When rat BMSCs were cultured in endothelial induction medium containing EGM-2 or endothelial induction medium + LBP. In the induction group without LBP, rat BMSCs had changed into endothelial-like cells after 10 days of induction. However, these induced by medium containing LBP should change into endothelial-like cells by 12 days. These indicated that LBP can delay the differentiation of EGM-2-induced rat BMSCs into endothelial lineage cells.

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