BACKGROUND: To improve the cell affinity of polylactic acid, the three-dimensional polylactic acid scaffold is made after pretreatment with bone morphogenetic protein 7 (BMP7).
OBJECTIVE: To evaluate the compatibility of BMP7-pretreated three-dimensional polylactic acid scaffold with
periosteum-derived cells.
METHODS: Passage 3 human periosteum-derived cells undergoing osteogenic induction at a density of 1×109/L were seeded into culture media containing the three-dimensional polylactic acid scaffold (experimental group) or seeded into simple culture media (control group). In the experimental group, the scaffold was pretreated with BMP7 for 24 hours. At 1, 3, 5, 7, 9, 11 days after culture, cell counting kit-8 method was used to measure cellular proliferation; at 3, 6, 9, 12 days, the cell-scaffold complex was observed under an electron microscope.
RESULTS AND CONCLUSION: In the two groups, the cells had an S-shaped growth curve. Compared with the control group, the cell proliferation in the experimental group was faster at the logarithmic phase, but slower at the stationary phase (P < 0.05). Under the transmission electron microscope, in the experimental group, the number of adherent cells was increased gradually, and the cells grew well with exuberant organelles at 3-6 days of co-culture, but cell aging and apoptosis were found at 9 days of co-culture. Under the scanning electron microscope, in the experimental group, the number of adherent cells on the scaffold surface gradually increased at 3-9 days of co-culture, and then cell apoptosis was notable at 12 days. These findings indicate that BMP7-pretreated three-dimensional polylactic acid scaffold has good cytocompatibility, and the optimal in vitro co-culture duration is 1 week.