Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (3): 418-422.doi: 10.3969/j.issn.2095-4344.2016.03.021

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Optimal dose and mechanism of sodium hyaluronate/carboxymethylcellulose for prevention of postoperative peritoneal adhesions: study protocol for a randomized controlled trial

Wu Zhi-min1, 2, Cui Hong-li2 , Liu Guo-hui3   

  1. 1Center for Gastrointestinal Cancer, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Beijing 100142, China; 2Department of General Surgery, Beijing Chuiyangliu Hospital, Beijing 100022, China;3Department of Emergency Surgery, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China
  • Received:2015-11-23 Online:2016-01-15 Published:2016-01-15
  • Contact: Liu Guo-hui, Department of Emergency Surgery, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China
  • About author:Wu Zhi-min, M.D., Associate chief physician, Center for Gastrointestinal Cancer, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Beijing 100142, China
  • Supported by:

    the Scientific Research Project of Jilin Provincial Health Department, No. 2012s011

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Abstract:

BACKGROUND: Local application of barriers is an effective way to prevent postoperative peritoneal adhesions. In previous experiments, a patented anti-adhesion material, sodium hyaluronate/carboxymethylcellulose, has been prepared. This glue-like polymer material has good biocompatibility, and because of its fluidity and compressibility, it can wriggle with the organs and diffuse in the entire abdominal cavity, and thereby play alubricant role to separate the intestine and peritoneum and reduce tissue adhesion.
METHODS/DESIGN: A randomized controlled animal experiment has been designed. Animal models of peritoneal adhesion are made in New Zealand rabbits followed by injection of sodium hyaluronate/ carboxymethylcellulose at different concentrations and doses. The optimal concentration and dose of sodium hyaluronate/ carboxymethylcellulose is screened by comparing percentage of peritoneal adhesion between treatment and control groups. Anti-adhesion mechanism of sodium hyaluronate/carboxymethylcellulose is explored through the detection of fibroblast growth factor, endothelial cell growth factor, transforming growth factor β and serum tissue-type plasminogen activator, urokinase-type plasminogen activator and fibrinogen.
DISCUSSION: This study will be powered to provide experimental evidence for sodium hyaluronate/carboxymethylcellulose in clincial prevention of postoperative peritoneal adhesion, as well as feasible ideas for exploring the anti-adhesion mechanism of sodium hyaluronate/carboxymethylcellulose.
ETHICAL APPROVAL: This study was approved by the Ethics Committee of the First Hospital of Jilin University. The disposal of animals was in line with the Guideline for the Care and Use of Laboratory Animals formulated by the Ministry of Science and Technology, China in 2006 and the Ethical Issues in Animal Experimentation released in 2009