In vitro cytotoxicity of SuperBond C&B, a resin cement for dental bonding

doi:10.3969/j.issn.2095-4344.2016.03.007

Abstract

Abstract:

BACKGROUND: Because of long-term contact with tooth tissues, gingival tissues and saliva in the mouth, oral bonding materials must have the characteristics of biological safety and low or no cytotoxicity, so as to ensure no irritation response to the oral mucous and no toxicity and allergic reactions when contacting with human tissues.
OBJECTIVE: To observe the in vitro cytotoxicity of an oral bonding material, SuperBond C&B.
METHODS: The logarithmic human periodontal ligament fibroblasts were obtained and inoculated in 96-well plates with a cell concentration of 1.0×108/L, 100 μL/hole. Supernatant was removed after 24 hours of routine culture, and the specimens were grouped and cultured. 90 μL DMEM culture liquid+10 μL SuperBond C&B were added in the experimental group. 100 μL medium was added in the negative control group. 100 μL phenol solution was added in the positive control group. After 72 hours of culture, the cell survival rate in each group was calculated by trypan blue exclusion test.
RESULTS AND CONCLUSION: The cell survival rates of the experimental group and the negative control group were higher than that of the positive control group (P < 0.05), and there was no significant difference between the experimental group and the negative control group (P > 0.05). These results show that SuperBond C&B has no cytotoxicity.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Jiang Xian-min, Zhao Xi . In vitro cytotoxicity of SuperBond C&B, a resin cement for dental bonding[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(3): 341-345.

Figures/Tables 3

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