Table of Content

01 April 2012, Volume 16 Issue 14 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Reference Manager ProCite BibTeX RefWorks

Toggle Thumbnails

Select

Induced differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by four inductors    Liu Bo-wu, Lü An-lin, Huang Wei, Hou Jing, Hou Zhao-lei, Hou Hong, Da Jing, Yang Na 2012, 16 (14):  2471-2476.  doi: 10.3969/j.issn.1673-8225.2012.14.001 Abstract ( 216 )   PDF (725KB) ( 326 )   Save BACKGROUND: It has been rarely reported that different inductors are used to induce bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells.  OBJECTIVE: To investigate the effects of 5-azacytidine, angiotensin Ⅱ, bone morphogenetic protein 2, pifithrin-α on induced differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells.  METHODS: Passage 3 rat bone marrow mesenchymal stem cells were divided into five groups: control, 5-azacytidine, angiotensin Ⅱ, bone morphogenetic protein 2, pifithrin-α. Surface antigen expression of bone marrow mesenchymal stem cells was identified by flow cytometry. After 4 weeks of induction, Cx43 and troponin Ⅰ expression was detected by western blot method and calcium transient capability of induced bone marrow mesenchymal stem cells was detected by laser confocal scanning microscopy with flou3/AM. RESULTS AND CONCLUSION: Cell surface antigen CD29, CD44, CD45 positive rate was 89.1%, 90.4%, 1.9% respectively. Troponin Ⅰ expression appeared in each group and the expression was significantly higher in the pifithrin-α group (P < 0.05), as well as significantly lower in the bone morphogenetic protein 2 group (P < 0.05), compared with the remaining groups. Cx 43 expression appeared in each group, and the expression was significantly lower in the control group (P < 0.05) compared with the remaining groups. In the four experimental groups, Cx43 expression was highest in the pifithrin-α group and lowest in the angiotensin Ⅱ group (P < 0.05). Calcium transient capability determined by fluorescence intensity was arranged as follows: angiotensin Ⅱ group > pifithrin-α group > 5-azacytidine group > bone morphogenetic protein 2 group > control group (P < 0.01). These findings suggest that after induced by 5-azacytidine, angiotensin Ⅱ, bone morphogenetic protein 2, and pifithrin-α, bone marrow mesenchymal stem cells differentiated towards cardiomyocyte-like cells and expressed myocardium-specific protein. Different inductors exert different effects on differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells which occurs because of different pathways. Related Articles | Metrics

Select

In vitro culture and biological characteristics of rabbit mandible-derived bone marrow mesenchymal stem cells    Li Song, Guo Yan-wei, Fang Dian-ji 2012, 16 (14):  2477-2480.  doi: 10.3969/j.issn.1673-8225.2012.14.002 Abstract ( 272 )   PDF (332KB) ( 305 )   Save BACKGROUND: Previous studies have demonstrated that bone marrow mesenchymal stem cells are harvested primarily from femur or ilium, but there have been few reports describing mandible-derived bone marrow mesenchymal stem cells. OBJECTIVE: To investigate in vitro isolation, culture and biological characteristics of rabbit mandible-derived bone marrow mesenchymal stem cells. METHODS: Bone marrow mesenchymal stem cells were harvested from rabbit mandible and isolated by density gradient centrifugation. Cells were adherently cultured in vitro, and passage 2 or 3 bone marrow mesenchymal stem cells were used for detection. Cell proliferation was detected by MTT assay. Fibroblast colony forming units were detected by examination of colony formation. The potential of bone marrow mesenchymal stem cells differentiation into osteoblasts and adipocytes was detected. RESULTS AND CONCLUSION: MTT assay showed that the cell growth curves of mandible-derived bone marrow mesenchymal stem cells exhibited incubation period, logarithmic phase and platform period. Colony-formation assays showed that cells exhibited the appearance of fibroblast colony forming units. After Aalizarin red and oil red O staining, there were mineralized nodules and lipid drops. These findings suggest that rabbit mandible-derived bone marrow mesenchymal stem cells show strong self-renewal and proliferative capacities and exhibit the potential of multi-directional differentiation. Related Articles | Metrics

Select

Effects of bone marrow mesenchymal stem cells on vascularization of hepatoma models of rats Song Hao1, Ji Wei-zheng2, Tai Qin-wen1, Shan Jiao-yu3, Zhang Jin-hui1 2012, 16 (14):  2481-2486.  doi: 10.3969/j.issn.1673-8225.2012.14.003 Abstract ( 229 )   PDF (705KB) ( 245 )   Save BACKGROUND: There are few reports about the effects of bone marrow mesenchymal stem cells (BMSCs) transplantation on vascularization during hepatocarcinogenesis in rats. OBJECTIVE: To observe the effects of BMSCs transplantation on vascularization during hepatocarcinogenesis in rats. METHODS: Thirty Wistar rats were divided into BMSCs transplantation group, pure model group and blank control group randomly. The first two groups were treated with diethylnitrosamine to prepare hepatoma models. The blank control group was treated with water as control. RESULTS AND CONCLUSION: Eight rats in the pure model group and nine rats in the BMSCs transplantation group were proved to be with liver cancer, and the SrY+ cells were seen in the BMSCs transplantation group. MRI showed that there were more space-occupying lesions in the liver of the BMSCs transplantation group compared with the pure model group (t = 2.45, P = 0.026). The average positive expression rate of vascular endothelial growth factor and microvessel density in the BMSCs transplantation group were higher than those in the pure model group (P < 0.05). In the hepatoma models of rats prepared with diethylnitrosamine, BMSCs can promote the vascularization resulting in the development of liver cancer. Related Articles | Metrics

Select

Rat bone marrow mesenchymal stem cells labeled and tracked with PKH26   Peng Yan, He Yuan-li, Zhu Shao-fang 2012, 16 (14):  2487-2390.  doi: 10.3969/j.issn.1673-8225.2012.14.004 Abstract ( 299 )   PDF (458KB) ( 309 )   Save BACKGROUND: Labeling of bone marrow mesenchymal stem cells (BMSCs) are important links to the research of differentiation and migration in vivo. OBJECTIVE: To label the BMSCs with PKH26 and to explore the effect of PKH26 labeling on the biological activity, differentiation in vitro and tracking in vivo. METHODS: BMSCs were isolated and cultivated from the bone marrow of rats. The passage 2 BMSCs were cultured and labeled with PKH26. The proliferation, cycle and apoptotic of cells in labeled group and unlabeled group were evaluated. The BMSCs in labeled group were performed with osteoblasts and lipoblasts induction in vitro. The PKH26-labeled BMSCs were transplanted through vein and the distribution of BMSCs in rat endometrium was observed under fluorescence microscope at 6 weeks after transplantation. RESULTS AND CONCLUSION: The influence of PKH26-labelled cells on cell proliferation, apoptotic and cycle was not significant. And the labeled cells still had biological characteristics of osteoblasts and lipoblasts. In endometrium, PKH26-labelled cells were mainly distributed in epithelial cells and stromal cells. It suggested that the PKH26 labeling method could be used to study the homing, plasticity and transplantation of BMSCs. Related Articles | Metrics

Select

In vitro osteogenic induction of rat bone marrow mesenchymal stem cells   Zhao Da-cheng, Wang Yu-liang, Dang Yue-xiu, Zhao Lin, Wang Jing, Wang Cui-fang 2012, 16 (14):  2491-2495.  doi: 10.3969/j.issn.1673-8225.2012.14.005 Abstract ( 195 )   PDF (423KB) ( 530 )   Save BACKGROUND: Bone marrow mesenchymal stem cells are a kind of good seed cells. Bone marrow mesenchymal stem cells compounded onto biological scaffold for treatment of bone defects have acquired satisfactory outcomes and become a gradually increasing research area. OBJECTIVE: To investigate the effect of in vitro osteogenic induction of rat bone marrow mesenchymal stem cells. METHODS: Sprague-Dawley rat bone marrow mesenchymal stem cells were in vitro isolated by adherent method. Passage 3 well growing bone marrow mesenchymal stem cells were divided into two groups. The control group was cultured only with DMEM/F12 culture medium. The experimental group was cultured with DMEM/F12 culture medium containing osteogenic inductor. RESULTS AND CONCLUSION: Primary bone marrow mesenchymal stem cells cultured by the whole bone marrow adherence method exhibited a shuttle-shaped or polygon-shaped appearance. After induced by osteogenic inductor, bone marrow mesenchymal stem cells adhered to plate wall in a circle or oval-shaped manner. Alkaline phosphatase activity was significantly higher in the experimental group than in the control group (P < 0.01). Calcified nodules appeared after alizarin red staining. Western blotting results showed that type Ⅰ collagen protein expression was significantly higher in the experimental group than in the control group (P < 0.01). ELISA quantitative analysis showed that osteocalcin was significantly higher in the experimental group than in the control group (P < 0.01). These findings suggest that whole bone marrow adherence method for culture of bone marrow mesenchymal stem cells is simple and practical and the cultured cells exhibit morphological and biological characteristics of osteoblasts after osteogenic induction. Related Articles | Metrics

Select

Salidrosides induced rat bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro Zhang Quan-wei1, 2, Zhao Xing-xu1, Zhao Hong-bin2, Zhang Ming2, Yang Yin-shu3, Ge Bao-feng2◇ 2012, 16 (14):  2496-2504.  doi: 10.3969/j.issn.1673-8225.2012.14.006 Abstract ( 240 )   PDF (1702KB) ( 347 )   Save BACKGROUND: Preliminary experiments have demonstrated that salidroside (SD) can induce bone marrow mesenchymal stem cells (BMSCs) to differentiate into neural-like cells. However, little is known about how SD affects BMSCs differentiation into neural-like cells. OBJECTIVE: To investigate the molecular mechanism by which SD induces BMSCs to differentiate into neural-like cells. METHODS: Passage 2 Wistar rat BMSCs were divided into a retinoic acid (RA) group, SD group, and a control group. Cells were observed at different time points. RESULTS AND CONCLUSION: The expression levels of neural stem cell marker nestin, neural cell differentiation-related to rabbit anti-microtubule protein and microtubule-associated protein 2 (MAP2) in the RA and SD groups were significantly higher than those in the control group at each time point (P < 0.01). The expression level of glial fibrillary acidic protein (GFAP) in the RA group was significantly higher than that in the SD group (P < 0.01). Real-time PCR results suggested that there were neuron-specific enolase (NSE) and MAP2 mRNA expressions in the RA and SD groups at each time point. The expression level depended but not absolutely on induction time. The high abundance of GFAP mRNA in the RA and SD groups appeared in the early stage of induction. NSE protein expression in the RA and SD groups was significantly increased with prolongation of induction time and rabbit anti-microtubule protein appeared in the early stage of induction. These findings suggest that SD, similar to RA, can promote BMSCs to differentiate toward neural cells, but it exhibits better effects on differentiation towards neural-like cells than RA.   Related Articles | Metrics

Select

Local injection of bone marrow mesenchymal stem cells versus triamcinolone acetonide for treatment of Achilles tendinitis in rabbits Qiu Chao, Hong Jian-jun, Mao Cheng-huang, Lu Xiao-lang 2012, 16 (14):  2505-2508.  doi: 10.3969/j.issn.1673-8225.2012.14.007 Abstract ( 266 )   PDF (344KB) ( 288 )   Save BACKGROUND: It remains to be disputed to apply long-lasting glucocorticoid for partial closure theray of Achilles tendonitis. OBJECTIVE: Bone marrow mesenchymal stem cells and long-lasting triamcinolone acetonide were respectively injected into the achilles tendon of a rabbit model of Achilles tendonitis to dynamically observe related indices and investigate the effects of bone marrow mesenchymal stem cells and long-lasting triamcinolone acetonide on tissue structure and mechanical performance of Achilles tendon with tendonitis. METHODS: Sixty rabbit models of unilateral Achilles tendinitis were randomly divided into four groups. Two groups were locally administered bone marrow mesenchymal stem cells and triamcinolone acetonide respectively once, and the remaining two groups were locally administered bone marrow mesenchymal stem cells and glucocortocoid respectively twice with a time interval of 2 weeks. RESULTS AND CONCLUSION: Compared with triamcinolone acetonide once group, rabbits in the bone marrow mesenchymal stem cells once or twice groups, rabbits exhibited regular, dense tendon bundles, increased fibroblasts, increased hydroxyproline content in the tendon tissue and increased maximum load strength of tendon (P < 0.05), in particular twice injections (P < 0.05). These findings suggest that local repeated injections of bone marrow mesenchymal stem cells exhibit better therapeutic effects than long-lasting triamcinolone acetonide application in treatment of Achilles tendinitis. Related Articles | Metrics

Select

Co-culture of autogenic bone marrow mesenchymal stem cells and allogenic chondrocytes optimizes seed cell source of cartilage tissue engineering  Xie Peng1, Zhang Zhong-wen2 2012, 16 (14):  2509-2514.  doi: 10.3969/j.issn.1673-8225.2012.14.008 Abstract ( 272 )   PDF (479KB) ( 310 )   Save BACKGROUND: There have been no cells that can meet the requirement of tissue engineering for seed cells. OBJECTIVE: To investigate the feasibility of co-culturing autogenic bone marrow mesenchymal stem cells and allogenic chondrocytes. METHODS: Rabbit chondorcytes were harvested by enzymatic digestion. Bone marrow mesenchymal stem cells were harvested by density gradient centrifugation and adherence methods. Passage 2 bone marrow mesenchymal stem cells and chondrocytes at a concentration of 3×108/L were randomly divided into three groups. Co-culture group: bone marrow mesenchymal stem cells were co-cultured with chondrocytes at a ratio of 2: 1. Experimental group: Chondrocytes of the same passage and concentration (concentration the same as the final concentration of co-culture group); control group: 1×108 /L chondrocytes (concentration the same as the final concentration of co-culture group). RESULTS AND CONCLUSION: The average population doubling time was 3 days in the co-culture group, 7 days in the experimental group, and 8 days in the control group. Cell proliferation was significantly faster in the co-culture group than in the experimental and control groups (P < 0.05). The glycosaminoglycan level was significantly higher in the co-culture group than in the experimental and control groups (P < 0.05). There was no obvious rejection in the co-cultures of autogenic bone marrow mesenchymal stem cells and allogenic chondrocytes. This suggests that autogenic bone marrow mesenchymal stem cells in cultures can promote the proliferation of chondrocytes and the synthesis of extracellular matrix, shorten culture time of chondrocytes, and reduce passages; at the same time, chondrocytes can promote oriented transformation of bone marrow mesenchymal stem cells towards chondrocytes, which save a large number of chondrocytes. Related Articles | Metrics

Select

In vitro culture, identification and osteogenic differentiation of human bone marrow mesenchymal stem cells Liu Wei1, Liu Meng2, Zhu Jing-song1, Hao Hong-wei1, Dong Ning-zheng2, Zhou Hai-bin1 2012, 16 (14):  2515-2519.  doi: 10.3969/j.issn.1673-8225.2012.14.009 Abstract ( 411 )   PDF (1326KB) ( 477 )   Save BACKGROUND: Research approaches and measurement index regarding osteogenic differentiation of bone marrow mesenchymal stem cells in vitro are not fully considered at home and abroad. OBJECTIVE: To establish and consummate a set of methods of isolating, culturing and identifying human bone marrow-derived mesenchymal stem cells (hBMSCs) and investigate their osteogenic differentiation ability in vitro. METHODS: hBMSCs were isolated using the method of density gradient centrifugation. The cell surface phenotypes of BMSCs were identified by flow cytometry. Passage 3 hBMSCs were induced by osteogenic culture medium for 2-3 weeks. RESULTS AND CONCLUSION: The primary cultured hBMSCs grew and proliferated vigorously after subculture. Passage 3 hBMSCs were positive for CD44, CD73, CD90 and were negative for CD34. After osteogenic induction, alkaline phosphatase activity was increased and cells were stained positive by Gomori, Von kossa and alizarin red. RT-PCR results showed the gene expression of collagen Ⅰ, alkaline phosphatase, osteocalcin, bone sialoprotein, osteopotin and osteonectin. This confirms that the primary hBMSCs can differentiate into osteoblasts in vitro successfully. These findings suggest that a stable, mature method of isolating, culturing and amplifying hBMSCs has been established. Related Articles | Metrics

Select

Three kinds of two-dimensional gel electrophoresis sample preparation methods of rabbit bone marrow mesenchymal stem cells   Qin Wan-an, Su Wei, Huang Zhao, Cui Xiang-rong, Guo Jie 2012, 16 (14):  2520-2523.  doi: 10.3969/j.issn.1673-8225.2012.14.010 Abstract ( 350 )   PDF (476KB) ( 287 )   Save BACKGROUND: A variety of “standard” sample preparation methods for two-dimensional gel electrophoresis have been reported, but there is no single one can be universally applied to rabbit bone marrow mesenchymal stem cells (rBMSCs). OBJECTIVE: To identif a suitable sample preparation method for rBMSCs two-dimensional gel electrophoresis (2-DE). METHODS: After full protein extraction of rBMSCs cultured in vitro, protein samples were divided into three groups: untreated, acetone precipitation and 2-D clean-up kit group, the protein concentration was measured by Bradford method, electrophoresis maps were analyzed. RESULTS AND CONCLUSION: Using 2-D clean-up kit to treat the protein sample could obtain the best electrophoresis map which showed lowest background, clearest protein spots, highest resolution and good reproducibility. Protein extraction treated with 2-D Clean-up was a more suitable 2-DE sample preparation method of rBMSCs. Related Articles | Metrics

Select

Effects of co-culture system on Bmi-1 expression in bone marrow mesenchymal stem cells Yang Rui-nian, Liu Liu, Wang Fu-ke, Zhao De-ping 2012, 16 (14):  2524-2529.  doi: 10.3969/j.issn.1673-8225.2012.14.011 Abstract ( 219 )   PDF (450KB) ( 290 )   Save BACKGROUND: Vascular endothelial cells can promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and provide nutritional support for growth and proliferation of BMSCs. OBJECTIVE: To investigate the effects of human umbilical vein vascular endothelial cells (hUVECs) in a co-culture system on morphology, growth and differentiation of human BMSCs, and Bmi-1 gene expression in cells. METHODS: Based on the co-culture system of cells, a simple culture group (hBMSCs group) and a combined culture group (BMSCs + hUVECs group) were established. On days 4, 6, 8, 10, cell morphology was observed and cells were counted for drawing cell growth curves; in addition, Bmi-1 gene expression in cells was detected in each group using real-time quantitative fluorescent PCR. RESULTS AND CONCLUSION: At each time point, compared with hBMSCs group, cells exhibited multiple morphological changes, some cells connected together in the latter stage, and osteogenic differentiation was more obvious in the BMSCs + hUVECs group. At each time point, cell number and Bmi-1 expression in the BMSCs + hUVECs group were significantly greater than in the BMSCs group. The BMSCs + hUVECs group showed a better compatibility compared with the hBMSCs group. These findings suggest that hUVECs promote the proliferation of hBMSCs in the co-culture system, and increase Bmi-1 expression in hBMSCs, thereby affecting aging and proliferative capacity of hBMSCs. Related Articles | Metrics

Select

Periurinary tract transplantation of autologous adipose-derived stem cells combined with fibroblasts for treatment of stress urinary incontinence Guan Li-ming, Li Xiu-juan, Chen Ya-ping, Li Qian, Wu Yun, Chen Wei 2012, 16 (14):  2530-2533.  doi: 10.3969/j.issn.1673-8225.2012.14.012 Abstract ( 285 )   PDF (323KB) ( 314 )   Save BACKGROUND: Stem cells proliferated and differented to form fibroblasts and well-suited connective tissue, followed by tissue regeneration, which is a gradually increasing research area for treatment of pelvic organ prolapse and stress urinary incontinence. OBJECTIVE: To investigate the feasibility of periurinary tract infection of autologous adipose-derived stem cells combined with fibroblasts for treatment of stress urinary incontinence. METHODS: Rats were induced to develop stress urinary transplantation by postpartum vaginal balloon dilation and bilateral ovariectomy. One month later, autologous adipose-derived stem cells and fibroblasts were injected into the region surrounding the urinary tract. Another month later, urinary voiding function was assessed by conscious cystometry. At the same time, urinary tract proximal end tissue was stained by hematoxylin-eosin and elastic fibers were stained to observe morphological change. RESULTS AND CONCLUSION: Compared with un-treated rats, rats receiving stem cell treatment exhibited increased leak point pressure (P < 0.01) with normal voiding, thickened urinary tract muscular layer (P < 0.01) and increased elastic fiber and smooth muscle content (P < 0.01). These findings suggest that periurinary tract infection of autologous adipose-derived stem cells combined with fibroblasts can be used for treatment of stress urinary incontinence because it can significantly increase abdominal leak point pressure and strengthen urinary tract muscular layer pressure. Related Articles | Metrics

Select

Stem cells transplanted with vascular endothelial growth factor 165 gene for improving the survival of tissue-engineered adipose Yang Bo, Shen Hong-liang, Xu Zhi-fei, Chen Kun, Li Song 2012, 16 (14):  2534-2539.  doi: 10.3969/j.issn.1673-8225.2012.14.013 Abstract ( 296 )   PDF (473KB) ( 385 )   Save BACKGROUND: During in vitro tissue engineering construction, poor blood supply in transplanted areas or delayed revascularization of implants leads to nutrition deficiency of seed cells, metabolism disorders, proliferation, differentiation and secretion dysfunction or even death, and at last causes the transplantation failure. OBJECTIVE: To investigate the feasibility of transfecting vascular endothelial growth factor 165 (VEGF165) gene into human adipose tissue-derived stem cells (ADSCs) for the neovascularization of tissue engineered adipose. METHODS: ADSCs were transfected by adenovirus-mediated VEGF165 gene, and transfected ADSCs combined with collagen sponge were transplanted into the back of nude mice as experimental group. Non-transfected ADSCs with collagen sponge was used as cell group, and DMEM culture medium+collagen sponge as control group. RESULTS AND CONCLUSION: Three months after transplantation, survival volume and capillary density of adipose tissue in the experimental group were higher than those of the cell and control groups, which in the cell group were higher than those of the control group (P < 0.01, P < 0.05). It is indicated that ADSCs can improve the neovascularization and survival volume of tissue-engineered adipose tissues and VEGF165 gene transfected ADSCs have a stronger ability of promoting the neovascularization of transplanted tissues. Related Articles | Metrics

Select

Comparison of ex vivo culture characteristics between human and rabbit adipose derived stem cells    Zhao Jian-hui1, Li Long2, Liang Li-hua3, Yi Cheng-gang1, Zeng Shu-hong1, Guo Shu-zhong1 2012, 16 (14):  2540-2544.  doi: 10.3969/j.issn.1673-8225.2012.14.014 Abstract ( 248 )   PDF (475KB) ( 337 )   Save BACKGROUND: Whether there are differences of different sources of adipose derived stem/stromal cells (ASCs) when cultured in vitro has been poorly understood. OBJECTIVE: To investigate the differences of ex vivo culture characteristics between human and rabbit ASCs under the same culture condition.  METHODS: Human ASCs (hASCs) were isolated from intact fat of abdomen harvesting of skin grafts. Rabbit ASCs (rASCs) were derived from subcutaneous fat tissue of back. The hASCs and rASCs were cultured and passaged in vitro and the morphology of the cells was observed. Passage 3 ASCs were used to compare the ability of growth and proliferation, identification of CD surface molecules and the ability of adipogenic and osteogenic differentiation. RESULTS AND CONCLUSION: Fibroblast-like adherent spindle-shaped cells could be isolated from both human and rabbit subcutaneous fat tissue. hASCs could passage after cultured for about 6-8 days, but rASCs were about 4-5 days. MTT results manifested that the growth climax of hASCs was the 4th day, whereas rASCs was the 6th day. Flow cytometry analysis showed the expression of CD29+CD31- could be seen both in human and rabbit ASCs. Both hASCs and rASCs had the cultural characteristics of stem cells. Compared with hASCs, rASCs had more powerful ability of proliferation and induction to fat whereas the ability of induction to bone was poor. Rabbit was a good choice of laboratory animal for fat transplantation research. Related Articles | Metrics

Select

In vitro osteogenetic effects of the Tet-On lentiviral vectors carrying human bone morphologic protein 2 transfected human umbilical cord mesenchymal stem cells  Zhang Zhen1, Tian Shao-qi1, Zhang Cai-long1, Sun Kang1, Zhang Ji-hua2, Sui Ai-hua3, Zhou Ming1, Feng Xue-tao 2012, 16 (14):  2545-2549.  doi: 10.3969/j.issn.1673-8225.2012.14.015 Abstract ( 282 )   PDF (527KB) ( 308 )   Save BACKGROUND: In addition to the advantages of conventional lentiviral vectors, Tet-On lentiviral vectors can regulate the expression of target gene by doxycycline or similar analogue because of insertion of trans-acting factors. OBJECTIVE: To investigate the effects of Tet-On lentiviral vectors carrying human bone morphologic protein 2 on transfection and osteogenesis of human umbilical cord mesenchymal stem cells (hUMSCs). METHODS: The cultured passage 3 hUMSCs were divided into four groups. In the hUMSC + doxcycline group, cells were stably transfected with Tet-On lentiviral vectors carrying human bone morphologic protein 2 and 10 mg/L doxcycline was added. In the hUMSC group, cells were treated the same as the hUMSC + doxcycline group, with the exception of addition of doxcycline. In the empty virus group, cells were transfected by green fluorescence protein. In the blank control group, cells were not treated. RESULTS AND CONCLUSION: Human bone morphologic protein 2 expression was significantly higher in the hUMSC + doxcycline group than that in the hUMSC group (P < 0.05). Human bone morphologic protein 2 expression was not detected in the empty virus and blank control groups. Alkaline phosphatase staining results showed that a large number of red or brown granules appeared in the cytoplasm in the hUMSC+ doxcycline group, and there were a few red or brown granules in the cytoplasm in the hUMSC group. Alkaline phosphatase activity was significantly higher in the hUMSC+ doxcycline group than in the hUMSC group (P < 0.05). Such red or brown granules were not observed in the empty virus and blank control groups. Tubercles developed in the hUMSC + doxcycline group and hUMSC group. The tubercles were more and larger in the hUMSC + doxcycline group compared with the hUMSC group. Tubercles were not observed in the empty virus and blank control groups. These findings suggest that Tet-On lentiviral vectors carrying human bone morphologic protein 2 can stably transfect human umbilical cord mesenchymal stem cells and significantly increase cellular osteogenic capability.   Related Articles | Metrics

Select

Effects of different implantation densities on the growth and multi-directional differentiation of human umbilical cord mesenchymal stem cells  Li Xin, Diao Ke, Zhang Feng-xiang, Sun Da-peng 2012, 16 (14):  2550-2554.  doi: 10.3969/j.issn.1673-8225.2012.14.016 Abstract ( 229 )   PDF (532KB) ( 296 )   Save BACKGROUND: It is important to search a rational cell implantation density to achieve stably and high efficiently growing stem cells during the culture of stem cells. OBJECTIVE: To investigate the effects of different implantation densities on primary culture of human umbilical cord mesenchymal stem cells and their differentiation into neuron-, islet-, and adipose-like cells. METHODS: Human umbilical cord venous endothelial and subendothelial layer cells were isolated and grouped according to different implantation densities for primary culture. The time for primary culture was recorded and cell passage was performed. Passage 1 human umbilical cord mesenchymal stem cells were in vitro induced to differentiate into neuron-, islet-, and adipose-like cells. RESULTS AND CONCLUSION: Human umbilical cord mensenchymal stem cells at an implantation density of 5×105-1 ×106/cm2 cell density showed optimal growth. After induced by β-sulfhydryl alcohol for 6 hours, human umbilical cord mesenchymal stem cells expressed nestin, neurofilament protein and glial fibrillary acidic protein. After induced by niacinamide, activin A, glucagon-like peptide 1 for 21 days, obvious islet-like cell masses appeared and were stained red by dithizone. After induced by adipogenic culture medium, cells were positive for oil red O staining, with appearance of obvious liquid drops. A large number of human umbilical cord mesenchymal stem cells could be quickly harvested at a proper implantation density and the cells could be induced to differentiate into neuron-, islet-, and adipose-like cells. Related Articles | Metrics

Select

Appropriate conditions for culture of umbilical cord blood mesenchymal stem cells   Zhang Tao1, Li Min2, Xu Pei-ru2 2012, 16 (14):  2555-2558.  doi: 10.3969/j.issn.1673-8225.2012.14.017 Abstract ( 246 )   PDF (376KB) ( 274 )   Save BACKGROUND: There have been many experiments describing culture of umbilical cord blood mesenchymal stem cells (UCBMSCs) in terms of improvement of medium compositions, separation method of cord blood cells, and the time for changing liquid for the first time. A safe and efficient training system has not been established yet. OBJECTIVE: To explore a better method for culture of UCBMSCs in vitro with different conditions. METHODS: The umbilical cord blood of healthy parturiens was taken from the Department of Obstetrics of the First Affiliated Hospital of Xinjiang Medical University. Several factors which influence the primary culture process of UCBMSCs were analyzed include the time for changing liquid for the first time, different culture mediums and different levels of fetal bovine serum. RESULTS AND CONCLUSION: Changing liquid at 5-7 days for the first time, low glucose DMEM or IMDE medium would be helpful to the growth of UCBMSCs. 30% fetal bovine serum and 20% fetal bovine serum did not produce different effects in culture of UCBMSCs. Related Articles | Metrics

Select

Isolation, culture and identitication of Ovis aries amnion epithelial cells Zhu Xue-min, Li Hai-jun, Mi Yan, Yang Yin-feng, Guan Hong-min, Cao Gui-fang 2012, 16 (14):  2559-2562.  doi: 10.3969/j.issn.1673-8225.2012.14.018 Abstract ( 240 )   PDF (395KB) ( 294 )   Save BACKGROUND: The amnion epithelial cells (AECs) with stem cells properties have been successfully obtained after isolating, digesting and culturing human and rat amnion and AECs have the potential to differentiate into cells of the endoderm (liver, lung, epithelium), mesoderm (bone, fat), and ectoderm (neural cells), OBJECTIVE: To obtain the Ovis aries AECs and detect the stem cell characteristics. METHODS: Ovis aries amnion tissue was peeled off by mechanical way and the low velocity-trypLE (pancreatic enzyme replacement) digestion method was used to isolate and culture the Ovis aries amnion, and the Ovis aries AECs were obtained. RESULTS AND CONCLUSION: The immunofluorescence observation showed that the labeled protein of AECs such as Oct-4, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 could be expressed on Ovis aries AECs. RT-PCR experiments confirmed that the genes of Oct-4, Sox-2 and Rex-1 were expressed and Nanog was not expressed. The results indicate that the Ovis aries AECs with stem cells properties have been achieved successfully. Related Articles | Metrics

Select

Effects of trypsogen and collagenase on culture of neural stem cells of fetal rat telencephalon   Liang Jun1, Song Xiao-yu2, Zhao Fu-sheng1, Li Yue-zhen1, Dong Jian-jiang1 2012, 16 (14):  2563-2566.  doi: 10.3969/j.issn.1673-8225.2012.14.019 Abstract ( 278 )   PDF (461KB) ( 336 )   Save BACKGROUND: Neural stem cells (NSCs) bring wide prospect for the study of neural development and repairing, and the in vitro culture is foundational to neuroscience. OBJECTIVE: To contrast the effects of trypsogen and collagenase on culture of NSCs in vitro. METHODS: The fetal rat telencephalons were isolated from SD rats pregnant for 14 days under sterile conditions, and tissues were divisively treated with trypsogen and typeⅠ collagenase contained EDTA following trituration, and cultured with serum-free medium. RESULTS AND CONCLUSION: The adherent monoculture NSCs were obtained using collagenase and congeries of cells were obtained using trypsogen. Nestin immunofluorescence identification showed the cells were positive, and the purity was over 99%. The primary adherent monoculture NSCs can be simply and quickly got using collagenase.   Related Articles | Metrics

Select

Effect of human umbilical cord blood mesenchymal stem cells transplantation on rotational behavior of Parkinson’s disease rats  Fan Zhi-gang, Liu Fang 2012, 16 (14):  2567-2570.  doi: 10.3969/j.issn.1673-8225.2012.14.020 Abstract ( 243 )   PDF (356KB) ( 352 )   Save BACKGROUND: To date, the clinical treatment of Parkinson’s disease (PD) mainly depends on drug, and as for cell transplantation experiment, bone marrow mesenchymal stem cells (BMSCs) transplantation is the common method. The reports about whether umbilical cord blood mesenchymal stem cells (UCBMSCs) transplantation can improve the rotational behavior are rare. OBJECTIVE: To explore the effect of human UCBMSCs transplantation on rotational behavior of PD rats. METHODS: The PD rat models were divided into the experimental group (n=20) and the control group (n=20). The fourth generation of MSCs were marked by Hoechst33258 and then transplanted into rat striatum in experimental group, and the rats in control group were given PBS. Apomorphine was injected intraperitoneally to examine the rotational behavior of the rats in two groups every week. Immunofluorescence double labeling method was used to identify the survival and migration of MSCs and the expression of glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE), tyrosine hydroxylase (TH) and synaptophysin at the 3rd, 6th and 9th weeks. RESULTS AND CONCLUSION: The rotational behavior of PD rats in experimetal group was improved obviously compared with control group (P < 0.05) after UCBMSCs transplantation. Part of MSCs could survive in brain tissue, and migrated to the striatum, callose and cortex with time prolonged and expressed GFAP, NSE and TH, but not expressed synaptophysin. UCBMSCs could obviously improve the rotational behavior of PD rats and were expected to be the seed cells to cure PD.   Related Articles | Metrics

Select

Bone marrow mesenchymal stem cells transplantation for treatment of dilated cardiomyopathy in rats  Xu Yan1, Zhang Yao2, Li Li-li2 2012, 16 (14):  2567-2580.  doi: 10.3969/j.issn.1673-8225.2012.14.022 Abstract ( 246 )   PDF (369KB) ( 271 )   Save BACKGROUND: Myocardial fibrosis induced by dilated cardiomyopathy (DCM) is the pathological basis of heart failure. At present, drug treatment, interventionaI therapy and surgical intervention cannot ameliorate necrotic myocardium and completely improve cardiac function． OBJECTIVE: To investigate the effect of allogenic bone marrow mesenchymal stem cells (BMSCs) transplantation on cardiac function and myocardial fibrosis in rats with DCM. METHODS: Forty Wistar rats were randomly divided into three groups: cell transplantation group (n=15), control group (n=15) and blank group (n=10). DCM models were established in cell transplantation group and control group. Four weeks after the models were established successfully, rats in cell transplantation group were injected with allogeneic BMSCs 150 μL (containing 3×106 cells). Rats in control group and blank group were also implanted with the culture medium in the same amount.  RESULTS AND CONCLUSION: Compared with the blank group, echocardiography showed that the left ventricular end-systolic inside diameter was increased before transplantation, while the ejection fraction and fractional shortening was decreased significantly (P < 0.01) in cell transplantation group and control group. Four weeks after transplantation, the echocardiography showed that the left ventricular end-systolic inner diameter was decreased, while the ejection fraction and fractional shortening was increased obviously (P < 0.01) in cell transplantation group compared with before transplantation. Expression of cardiac collagen in cell transplantation group was lower than that in control group (P < 0.05). Compared with the control group, the expression of matrix metalloproteinase 2 and matrix metalloproteinase 9 was significantly increased in the other two groups (P < 0.05). In conclusion, bone marrow mesenchymal stem cells (BMSCs) transplantation can improve cardiac function and ameliorate myocardial fibrosis in rats with DCM. Related Articles | Metrics

Select

Expression of tongue cancer stem cells marker CD44, ESA and CXCR4 in different environments Huang Ying-hua1, Yao Jin-guang2, Kuang Xiao-cong3, Cai Jie4, Xie Ji-sheng5, Li Jun2, Chen Hai-bo2, Yang Yong-rong2 2012, 16 (14):  2571-2575.  doi: 10.3969/j.issn.1673-8225.2012.14.021 Abstract ( 279 )   PDF (508KB) ( 234 )   Save BACKGROUND: Cancer stem cells marker expression in different environments differ greatly because the microenvironment can impact the expression of proteins. OBJECTIVE: To discuss whether different cultures in vitro have influence on the expressions of cancer stem cells marker CD44, ESA and CXCR4. METHODS: Tongue cancer stem cells TCA8113 cell line was obtained. In vitro, three different microenvironments were established: stem cell culture medium, conventional standard medium and conventional standard medium supplemented with adriamycin. The expressions of three proteins in TCA8113 tongue carcinoma cells were detected by immunohistochemistry (IHC) and flow cytometry (FCM). RESULTS AND CONCLUSION: In all microenvironments, IHC showed the expressions of CD44 and ESA were “++”. In stem cell culture medium and the other microenvironments, the expressions of CXCR4 were “±” and “+” respectively. FCM found the expressions of CD44 and ESA were above 90% in all microenvironments; compared with conventional standard medium, the expression of CXCR4 was super low in stem cell culture medium, and recovery when tongue cancer cells reversed into conventional standard medium from stem cell culture medium; the expression of CXCR4 was increased in conventional standard medium supplemented with adriamycin. The expressions of cancer stem cell markers were various in different mircoenvironments; it needs to take various markers into account and combine with the experessions of markers on cancer cells in vivo to identify cancer stem cell markers. Different kinds of cancer stem cells may accumulate together by different microenvironments model. Related Articles | Metrics

Select

Tail vein transplantation of human umbilical cord mesenchymal stem cells for treatment of cerebral ischemia-reperfusion injury in rats   Zhang Pei-pei1, Liu Hui-chun1, Yan Xiao-ling2, Wang Shi-min2, Kong Fan-ming2, Zhang Wen-zhi2 2012, 16 (14):  2581-2584.  doi: 10.3969/j.issn.1673-8225.2012.14.023 Abstract ( 321 )   PDF (397KB) ( 308 )   Save BACKGROUND: The specific mechanism of tail vein transplantation of stem cells through the blood-brain barrier to reach the host brain damage region is not clear. OBJECTIVE: To evaluate the safety of tail vein transplantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) for the treatment of cerebral ischemia-reperfusion injury in Sprague-Dawley (SD) rats and to investigate its possible mechanism. METHODS: hUC-MSCs were isolated using density gradient centrifugation combined with adherent screening method, and then labeled with BrdU. SD rats were used to establish the middle cerebral artery ischemia-reperfusion model with modified suture method. Rats of transplantation group A at 7 days after injury and group B at 14 days were given hUC-MSCs through the tail vein. Rats in the model group were left intact.  RESULTS AND CONCLUSION: (1) The mNSS score of transplantation group A and group B was lower than that of model group after transplantation for 7, 14, 28, 35, 42, 49 and 56 days (P < 0.05), and the mNSS score of transplantation group A was lower than that of transplantation group B after transplantation for 7, 14, 28, 35, 42 and 49 days (P < 0.05). The mNSS score reached the plateau phase at 35 days in model group, 42 days in transplantation group A and 49 days in transplantation group B. (2) Brdu+Nestin, Brdu+microtubule-associated protein 2, Brdu+glial fibrillary acidic protein, Brdu+factor Ⅷ and Brdu+vascular endothelial growth factor immunohistochemical double staining positive cells could be seen at the center of damage site in rats of the group A and group B, there were no CD11b and MPO immunohistochemical single staining positive cells. hUC-MSCs transplantation through tail vein is able to survive in the host brain damage region and improve the recovery of the neural function of ischemic brain injury rats. The mechanism may be related to the transplanted cells differentiating into neuron-like and glial-like cells, which promote the angiogenesis and secret the neurotrophic factors. In this study, no obvious immune rejection and tumor cells were found, and the transplantation has high safety. Related Articles | Metrics

Select

Short-term effects of human umbilical cord-derived mesenchymal stem cells in treatment of patients with decompensated cirrhosis    Zhang Yue-fan1, Li Nan2, Zhai Jun-shan2, Jiang Li-jun2, Cao Jian-ning3 2012, 16 (14):  2585-2588.  doi: 10.3969/j.issn.1673-8225.2012.14.024 Abstract ( 240 )   PDF (340KB) ( 336 )   Save BACKGROUND: Umbilical cord-derived mesenchymal stem cells (UCMSCs) can differentiate into hapatocyte-like cells. OBJECTIVE: To investigate short-time clinical effects of UCMSCs transplantation in treatment of patients with decompensated cirrhosis. METHODS: Thirty patients with decompensated cirrhosis were involved. Eighteen patients in the control group were treated with conventional therapy, while twelve patients in the transplantation group were treated with UCMSCs transplantation via hepatic artery based on conventional therapy. RESULTS AND CONCLUSION: After transplantation, albumin level in the transplantation group showed an ascend trend and had a significant increase (P < 0.05) compared with the control group from 2 weeks to 12 weeks of transplantation. Meanwhile, both alanine aminotransferase and prothrombin time levels began to decrease at 2 weeks after transplantation and were significantly lower than those of the control group (P < 0.05) from 8 weeks to 12 weeks of transplantation. In contrast to the controls, the level of total bilirubin in the transplantation group showed an ascend trend and had a significant increase (P < 0.05) from 4 weeks to 12 weeks of transplantation. The differences in clinical symptoms and signs between the two groups were not significant (P > 0.05). The transplantation of UC-MSCs via hepatic artery is effective in short time for treatment of patients with decompensated cirrhosis. Related Articles | Metrics

Select

null Shi Ying-ying, Li Yong-ping, Zhang Wen-xin, Zheng Jian-liang, Lin Jian-xian 2012, 16 (14):  2589-2592.  doi: 10.3969/j.issn.1673-8225.2012.14.025 Abstract ( 268 )   PDF (403KB) ( 268 )   Save References | Related Articles | Metrics

Select

Cytoplasm vacuolization of fibroblasts during purification of Schwann cells by geneticin (G418): An optical microscope observation and analysis Chen Bao, Shi Xiao-yuan, Xiang Ning, Wang Guang-lin 2012, 16 (14):  2593-2596.  doi: 10.3969/j.issn.1673-8225.2012.14.026 Abstract ( 266 )   PDF (297KB) ( 273 )   Save BACKGROUND: Geneticin (G418) combined with differential adhesion method and differential detachment method are used to eliminate the contaminated fibroblasts in the purification of Schwann cells. Most of the researches prefer to describe purity of Schwann cells, rather than give a description of cell morphology change and its probable cause during the purification process.  OBJECTIVE: To describe the pathological changes of fibroblasts through the morphological photos of purified fibroblasts and Schwann cells at different time points taken by optical microscope. METHODS: Schwann cells were isolated from sciatic nerves of rats by geneticin (G418) combined with differential adhesion method and differential detachment method in primary cultivation. The cultivated cells were divided into two groups. In the experimental group, the cells were transferred to another 6-well plate after once differential adhesion, and the waste product accumulated medium was changed to fresh basal medium every 2 days, the differential detachment method was used to passage. In the control group, cells were given differential detachment once again and transferred to another 6-well tissue culture plate, and the waste product accumulated medium was changed to fresh purification medium every 2 days, the differential detachment method was used to passage. Cytopathologic changes of fibroblasts in different periods were observed by optical microscope. RESULTS AND CONCLUSION: Cytopathologic changes of fibroblasts could not be observed under optical microscope in early stage of the purification, the proliferation of the fibroblasts was decreased and the cytoplasm vacuolization of fibroblasts was observed obviously after cells were sub-cultured for three times or more (three to four weeks). Fibroblast proliferation was restricted in early stage of the Schwann cells purification process by geneticin (G418), but cytoplasm vacuolization of fibroblasts delayed to the third generation or more. Related Articles | Metrics

Select

Neurotrophic factors participate in proliferation and differentiation of neural stem cells: literature retrieval results based on Web of Science   Jiang Zhen 2012, 16 (14):  2597-2606.  doi: 10.3969/j.issn.1673-8225.2012.14.027 Abstract ( 288 )   PDF (400KB) ( 323 )   Save BACKGROUND: Neurotrophic factors have a close relationship with proliferation and differentiation of neural stem cells and play an important role in induced differentiation of neural stem cells. OBJECTIVE: To identify the global research trends of effects of neurotrophic factors on proliferation and differentiation of neural stem cells via a bibliometric analysis of Web of Science. DESIGN: A bibliometric study. DATA RETRIEVAL: We performed a bibliometric analysis for data retrievals regarding effects of neurotrophic factors on proliferation and differentiation of neural stem cells from 2002 to 2011 via Web of Science. SELECTION CRITERIA: Inclusive criteria: Peer-reviewed articles about effects of neurotrophic factors on proliferation and differentiation of neural stem cells which were published and indexed in Web of Science, including articles of original research articles, reviews, meeting abstracts and proceeding paper. Exclusive criteria: articles need to be manually searched or accessed only through telephone; unpublished articles; correction paper. MAIN OUTCOME MEASUREMENTS: Total article outputs; type of articles; distribution of output in subject categories; publication distribution of countries; publication distribution of institutions; top cited paper; distribution of publications. RESULTS: From 2002 to 2011, 257 papers studying effects of neurotrophic factors on proliferation and differentiation of neural stem cells were indexed in Web of Science, including 88 papers addressing nerve growth factor on proliferation and differentiation of neural stem cells, 127 papers addressing brain-derived neurotrophic factor on proliferation and differentiation of neural stem cells, and 42 papers addressing neurotrophin 3 on proliferation and differentiation of neural stem cells. There were only 7 articles published in 2002, whereas the number of publications doubled since 2007. Totally 43 articles addressing neurotrophic factors on proliferation and differentiation of neural stem cells were published and covered in 2010, which reach a peak. Original research was the most frequently document type of published papers, which 225 original research paper were indexed and published in the past 10 years. The result showed that nearly one third of the literatures in the field were published by Americans institutes/universities. China was ranked No. 2 in terms of number of literature published. It is interesting that most cited articles were mostly published in Cell Transplantation, Journal of Neuroscience Research, Neuroscience Letters and Brain Research. CONCLUSION: From the analysis of literature and research trends, we found that the research of effects of neurotrophic factors on proliferation and differentiation of neural stem cells is becoming more mature, and the number of literatures in this field is increasing. It is the research hot in recent years.   Related Articles | Metrics

Select

Literature analysis of neural stem cells transplantation for the treatment of spinal cord injury in the past 10 years based on Science Citation Index database Yuan Ning1, Tian Wei1, Chen Da-fu2, Sun Lei2, Yuan Run-ying2 2012, 16 (14):  2607-2616.  doi: 10.3969/j.issn.1673-8225.2012.14.028 Abstract ( 273 )   PDF (479KB) ( 401 )   Save BACKGROUND: Spinal cord injury is a serious central nervous system trauma. Tissue engineered-cell transplantation provides a new approach for spinal cord injury, which make it possible for repairing or regenerating the injured axon and recovering partial of spinal functions. OBJECTIVE: To multivariately analyze the literature on neural stem cells transplantation for the treatment of spinal cord injury through Science Citation Index Database (SCI). DESIGN: Bibliometric analysis. DATA RETRIEVAL: A computer-based retrieval was performed for the literature of neural stem cells transplantation for treating spinal cord injury during 2002-01 and 2011-12 in SCI. The retrieval results were analyzed, and the trends were described in words and graphics. INCLUSION CRITERIA: Articles on neural stem cells transplantation for the treatment of spinal cord injury include the following types: (1) Peer-reviewed original paper; (2) reviews; (3) meeting notes and abstracts; (4) proceeding papers; (5) editorial materials; (6) letters. Exclusive criteria were included: (1) Articles unrelated the study of neural stem cells transplantation for the treatment of spinal cord injury. (2) Articles published before 2002. (3) Articles which were not published on journals. (4) Articles which need to be retrieved by phone or manually. MAIN OUTCOME MEASUREMENTS: The literatures were analyzed by type of document, authors, publication year, journal distribution, discipline distribution, national distribution, institutional information, citation frequency and the fund agencies. RESULTS: A total of 500 literatures on neural stem cells transplantation for the treatment of spinal cord injury were retrieved in SCI database, in which most of paper were published as original articles (n=348). Fifteen articles were identified as classic literature. The overall number of literature had an upward trend from 2002 to 2011. Journal of Neurotrauma published most papers in this field (n=22, 15.09%), followed by Journal of Neuroscience Research (n=21). CONCLUSION: This paper provides a valuable reference for researchers to understand the overview and present situation of this field in order to set further research.  Related Articles | Metrics

Select

Bone marrow stromal stem cells repair knee meniscus injury Li Jie1, Liu Fu-shun2 2012, 16 (14):  2617-2620.  doi: 10.3969/j.issn.1673-8225.2012.14.029 Abstract ( 317 )   PDF (416KB) ( 299 )   Save BACKGROUND: Bone marrow stromal stem cells transplantation has become one of the most perspective methods to solve the problem of cartilage tissue defects, and bone marrow stromal stem cells have become the important seed cells to repair the cartilage tissue defects. OBJECTIVE: To analyze the application of bone marrow stromal stem cells on meniscus injury and cartilage injury, in order to provide the theory reference for the repair and treatment of knee meniscus injury. METHODS: The PubMed database and Tongfang database 1997/2011 were retrieved by the first and second authors with the key words “Bone Marrow Stromal Cells; Knee Joint Meniscus Injury” in English and Chinese. A total of 26 articles related to bone marrow stromal stem cells for repair of the knee meniscus injury were included to analysis. RESULTS AND CONCLUSION: Bone marrow stromal cells in the repair and treatment of knee meniscus injury have unique advantages, including less damage surface, convenient and easy to operate, no complications, and no changes in morphology and biological function caused by the removal of the meniscus, and without load conduction disorders and caused by allogeneic material transplantation and the possibility of late onset osteoarthritis. The treatment concept of bone marrow stromal stem cells transplantation was close to meniscus injury, and became the new ideas in the current and future treatment and repair of knee meniscus injury. During the process of repair treatment, the key was how to obtain a large amount of purified bone marrow stromal stem cells and induce into the meniscus cartilage phenotype in vitro. The exact mechanism underlying induction of differentiation should be further investigated. Related Articles | Metrics

Select

Application and prospects of bone marrow mesenchymal stem cells and cytokines in the treatment of femoral head necrosis  Zuo Si-li, Gong Yue-kun 2012, 16 (14):  2621-2624.  doi: 10.3969/j.issn.1673-8225.2012.14.030 Abstract ( 232 )   PDF (390KB) ( 333 )   Save BACKGROUND: Simple application of bone marrow mesenchymal stem cells (BMSCs) to treat femoral head necrosis has a poor effect, Addition of cytokines during BMSCs application plays a better role. OBJECTIVE: To summarize the application of BMSCs induced by related cytokines on the treatment of femoral head necrosis. METHODS: The PubMed database and Wanfang database from 1989 to 2009 were searched by the first author. The articles were related to the new progress in the treatment of femoral head necrosis, the application of BMSCs on the treatment of bone-related diseases, the related cytokines which could induce BMSCs to differentiate into osteoblasts and the underlying mechanism. RESULTS AND CONCLUSION: The cytokines such as bone morphogenetic proteins, osteogenic growth peptide, vascular endothelial growth factor, cartilage-derived morphogenetic protein 1, transforming growth factor β, insulin-like growth factor-1, platelet-derived growth factor could induce the BMSCs to differentiate into osteoblasts and chondrocytes, and could promote the recovery of callus. Thus, the clinical application of cytokine induced BMSCs could optimize the treatment of femoral head necrosis.  Related Articles | Metrics

Select

Autologous bone marrow stem cells transplantation in the treatment of lower limb ischemia Yu Gui-ping, Wang Zhong 2012, 16 (14):  2625-2628.  doi: 10.3969/j.issn.1673-8225.2012.14.031 Abstract ( 221 )   PDF (410KB) ( 497 )   Save BACKGROUND: Bone marrow stem cells can promote the ischemic limb angiogenesis, the formation of new collateral vessels, and improve local blood supply through a variety of mechanisms. OBJECTIVE: To review the effect of bone marrow stem cells transplantation in the treatment of lower limb ischemia. METHODS: PubMed and CNKI were retrieved using computer to search relevant articles published from December 2001 to December 2011, and the keywords were “bone marrow stem cells, lower extremists ischemia”. RESULTS AND CONCLUSION: Bone marrow stem cells can migrate to the site of lower limb ischemia, and participate in tissue regeneration, formation and secretion of many vasoactive growth factors to promote angiogenesis, promote cell proliferation and differentiation, which are conducive to tissue and cell regeneration. However, there are still many questions to be solved, such as preservation of seed cells, regulatory mechanism of cell differentiation and proliferation in vitro, biomechanical properties of skeletal muscle cells, cellular and molecular biological characteristics of cells in repaired tissues. Related Articles | Metrics

Select

Research progress about neural stem cells transplantation for treatment of infantile cerebral palsy   Zhang Zhi-you1, Chen Gang2 2012, 16 (14):  2629-2632.  doi: 10.3969/j.issn.1673-8225.2012.14.032 Abstract ( 300 )   PDF (402KB) ( 380 )   Save BACKGROUND: Neural stem cells transplantation can be used to cure cerebral palsy (CP). OBJECTIVE: To summarize the investigative situation and new progress about neural stem cells transplantation for the treatment of CP. METHODS: A computer-based research was performed on the CNKI database and PubMed database (1999-01/2011-04) to search the related articles about neural stem cells transplantation for CP. The key words were “cerebral palsy, neural stem cell, transplantation” in English and Chinese. The articles related to the neural stem cells transplantation for CP was selected, and for the articles in the same field, we selected the articles published recently or in the authorized journals. There were 119 articles after the initial survey. Then 28 articles related to neural stem cells transplantation were selected according to the inclusion criteria. RESULTS AND CONCLUSION: Neural stem cells transplantation combined with neurenergen 3 could promote the recovery of the function in the child with CP and point out the direction for the future treatment of CP.   Related Articles | Metrics

Select

Immunoregulation and human umbilical cord blood-derived mesenchymal stem cells transplantation Jiao Bao-liang1, Wang Jing-chuan1, Gao Bing-hua2, Wang Xin-sheng1 2012, 16 (14):  2633-2636.  doi: 10.3969/j.issn.1673-8225.2012.14.033 Abstract ( 266 )   PDF (393KB) ( 218 )   Save :BACKGROUND: Research in recent years suggests that the self-renewal and multi-directional differentiation potency of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) offer basic condition to cell transplantation treatment. Moreover, their immunoloregulation function enormously expands the direction and limits cell transplantation treatment. OBJECTIVE: To retrospectively analyze the immunoloregulation and human UCB-MSCs transplantation. METHODS: The key word “umbilical cord blood-derived mesenchymal stem cells” was used to search in Pubmed database and CNKI database from January 2008 to June 2011 in English and Chinese using computer. The preliminary screening was made through reading the title and abstract. The articles with unrelated contents, repetitive and Meta analysis were excluded. 30 papers of pertinent literature to be published in the near future or published in the authority magazine were selected to review. RESULTS AND CONCLUSION: Human UCB-MSCs have the similar self-renewal and multi-directional differentiation potency with the bone marrow derived mesenchymal stem cells. Through cell transplantation technique, human UCB-MSCs show powerful potentiality in diabetes mellitus treatment, neural degeneration disease like Alzheimer’s disease and Parkinson’s disease and injury of nerve retreatment. Meanwhile, human UCB-MSCs have immunoregulatory ettects, they can lower immune reaction through down regulation of T-cells. We also get some advancements on several immunological diseases such as cell therapy of graft versus host disease and lupus nephritis.   Related Articles | Metrics

Select

Research progress in brain tumor stem cells Wang Xin-xin1, Liu Ji-ping2 2012, 16 (14):  2637-2640.  doi: 10.3969/j.issn.1673-8225.2012.14.034 Abstract ( 255 )   PDF (322KB) ( 254 )   Save BACKGROUND: Research in recent years has discovered some stem cell-like cells exist in brain tumors, which have the properties of endless cell proliferation, uncontrolled self-renewal and multi-directional differentiation, and they are called as brain tumor stem cells. Brain tumor stem cells play a key role in the progress of tumorigenesis, growth, invasion, metastasis and recurrence. OBJECTIVE: To conclude and explore the current studies on brain tumor stem cells. METHODS: A computer-based search of Pubmed Database was performed to retrieve relevant articles about brain tumor and brain tumor stem cells published from January 1977 to July 2011. Books on stem cells and brain tumor stem cells were also retrieved. The data were selected primarily, and 32 articles related to brain tumor stem cells were selected. RESULTS AND CONCLUSION: Brain tumor stem cells exist in malignant brain tumor, and they are the origin of the occurrence, development, metastasis and recurrence of malignant brain tumor. Brain tumor stem cells express CD133, Nestin protein and ABC transporter in malignant brain tumors. Recently, a simple method has been obtained to isolate brain tumor stem cells. The proportion of CD133 positive tumor stem cells is positively correlated with the severity of malignant brain tumors, which can be used as a diagnostic indicator of prognosis. Related Articles | Metrics

Select

Relationship between vitamin D, vitamin D receptor and hair Zhuo Na1, Xin Yan2, Guo Guo-xiang1 2012, 16 (14):  2641-2644.  doi: 10.3969/j.issn.1673-8225.2012.14.035 Abstract ( 378 )   PDF (363KB) ( 397 )   Save BACKGROUND: Increasing evidence has demonstrated that vitamin D and its receptor correlate with hair. OBJECTIVE: To fully discuss the relationship between vitamin D, vitamin D receptor and hair follicle stem cells, hair cycle, signal transduction and alopecia. METHODS: A computer online retrieval was performed in PubMed and CNKI database to search papers addressing the relationship between vitamin D, vitamin D receptor and hair published between November 1990 and November 2011 with the key words “ vitamin D, vitamin D receptor, hair, alopecia, gene polymorphism” in Chinese or English. Papers published recently or in the authoritative journals were selected. RESULTS AND CONCLUSION: A total of 152 papers were initially retrieved. According to inclusion and exclusion criteria, 30 papers were selected. The current studies have shown that vitamin D and its receptor play an important role in hair and the disorder of vitamin D would lead to alopecia. Regulation of vitamin D and its receptor may be successful in preventing and treating hair disorders and is promising in future use.   Related Articles | Metrics

Select

Treatment of nine patients with amyotrophic lateral sclerosis by autologous peripheral blood mononuclear cells transplantation: change of short-term effect   Sun Da-yong, Han Jie, Li Xue-jiao, Liu Jing, Song Chun-li 2012, 16 (14):  2645-2647.  doi: 10.3969/j.issn.1673-8225.2012.14.036 Abstract ( 508 )   PDF (272KB) ( 389 )   Save BACKGROUND: At present, there are some clinical reports about the treatment of amyotrophic lateral sclerosis (ALS) by transplantation of autologous bone marrow nucleated cells, olfactory cells and umbilical cord blood-derived stem cells. OBJECTIVE: To evaluate clinical effect of peripheral blood mononuclear cells transplantation on the treatment of 9 ALS patients. METHODS: The changes of neurological function (Norris, FIM, Bathel score) and electromyogram (EMG) changes of 9 ALS patients were evaluated by before-after self control study at the 1st, 3rd, 6th months after peripheral blood mononuclear cells transplantation. RESULTS AND CONCLUSION: After peripheral blood mononuclear cells transplantation, the symptom was improved, neurological scores were significantly higher than pre-transplant, short-term effect was obvious and the EMG showed improvement trend. The results showed that peripheral blood mononuclear cells transplantation has valid short-term effect on improving the neurological dysfunction of ALS patients. Related Articles | Metrics

Select

Detection of interleukin 2, interleukin 10, transforming growth factor β and CD4+CD25+ regulatory T cells in peripheral blood of patients with acute lymphoblastic leukemia  Wu Cui-Ping1, Qin Xi1, Wu Cui-yun2, Zhu Hong1, Zhou Hai-yan1 2012, 16 (14):  2648-2651.  doi: 10.3969/j.issn.1673-8225.2012.14.037 Abstract ( 301 )   PDF (356KB) ( 316 )   Save BACKGROUND: At present, the cause of acute lymphoblastic leukemia (ALL) has not yet entirely understood, CD4+CD25+ regulatory T cells (Treg) may have certain regulatory effect on immune response in pathogenic process of ALL. OBJECTIVE: To study the levels of interleukin 2 (IL-2), interleukin 10 (IL-10), transforming growth factor (TGF-β) of CD4+CD25+ regulatory T cells (Treg) in peripheral blood of patients with ALL. METHODS: The peripheral blood mononuclear cells (PBMC) were isolated with density gradient centrifugation from 22 B cell series ALL (B-ALL) patients, 13 T cell series ALL (T-ALL) patients and 18 normal people. Immunomagnetic bead separation was used to separate CD4+CD25+Treg cells, and the levels of IL-2, IL-10 and TGF-β in the cells culture supernatant were detected by using enzyme-linked immunosorbent assay (ELISA). RESULTS AND CONCLUSION: The levels of IL-10 and TGF-β in the cells culture supernatant of B-ALL patients and T-ALL patients were significantly higher than those in normal people, while the level of IL-2 was significantly lower than that in normal people (P < 0.05). There was no significant difference between B-ALL and T-ALL patients in the IL-2, IL-10 and TGF-β levels of the cells culture supernatant (P > 0.05). CD4+CD25+Treg cells might interfere the immunologic reaction of antitumor by increasing the IL-10 and TGF-β levels and decreasing the IL-2 level.   Related Articles | Metrics

Select

Transplantation of umbilical cord mesenchymal stem cells for treatment of autosomal dominant spinocerebellar ataxias in 12 cases** Qiu Yun1, Wang Zheng1, Lu Hong-she2, Xu Peng2, Chen Wen-yi2, Lu Yan-guang2, Ding Yong-hong2 2012, 16 (14):  2652-2655.  doi: 10.3969/j.issn.1673-8225.2012.14.038 Abstract ( 294 )   PDF (230KB) ( 307 )   Save BACKGROUND: There is no study addressing transplantation of umbilical cord mesenchymal stem cells for the treatment of spinocerebellar ataxia. OBJECTIVE: To study the clinical effect of human umbilical cord stem cells transplantation in the treatment of autosomal dominant spinocerebellar ataxias. METHODS: Spinocerebellar ataxias patients selected from Stem Transplantation Center of the 455 Hospital of Chinese PLA were treated with umbilical cord mesenchymal stem cells transplantation via intrathecal injection. The number of umbilical cord mesenchymal stem cells was 107 per transplantation, once per week, for 4 weeks. RESULTS AND CONCLUSION: Both the total score of the International Cooperative Ataxia Rating Scale and Activities of Daily Living score were significantly decreased at 1 month after transplantation compared with before treatment (P < 0.05). The nerve function was significantly improved and the total effective rate was up to 16.7%. Experimental findings indicate that, transplantation of umbilical cord mesenchymal stem cells via intrathecal injection is a feasible and effective treatment to ameliorate the clinical efficacy of spinocerebellar ataxias patients and improve their quality of life. Related Articles | Metrics

Select

Umbilical cord mesenchymal stem cell transplantation and rehabilitation treatment for cerebral hemorrhage sequela: 1 year follow-up  Yuan Jin-guo1, Feng Bin1, Cao Cang-zhu1, Han Shu-sheng1, Sun Yin-chen1, Liu Jian-ling2 2012, 16 (14):  2656-2660.  doi: 10.3969/j.issn.1673-8225.2012.14.039 Abstract ( 340 )   PDF (286KB) ( 660 )   Save BACKGROUND: Human umbilical cord mesenchymal stem cells exhibit the potential to differentiate into neural cells, which have been confirmed in in vivo and in vitro experiments. There have been few clinical reports describing umbilical cord mesenchymal stem cells for treatment of cerebral hemorrhage. OBJECTIVE: To investigate the therapeutic effects of human umbilical cord mesenchymal stem cell transplantation combined with rehabilitation treatment for cerebral hemorrhage sequela. METHODS: Forty-five patients with cerebral hemorrhage were assigned to two groups according to admission data: treatment group (n=22) and control group (n=23). After 10-18 days of routine treatment, each patient had sequela to different extents. The treatment group received human umbilical cord mesenchymal stem cell transplantation and rehabilitation treatment and the control group only received rehabilitation treatment. RESULTS AND CONCLUSION: All cases had been followed up for 12 months. Compared with before treatment, Fugl-Meyer scores and modified Barthel index were significantly higher at 1, 3, 6, 12 months after treatment in each group (P < 0.05). After treatment, Fugl-Meyer scores and modified Barthel index in the experimental group were significantly higher than in the control group (P < 0.05), and body movement function and daily life activities improved obviously. After 3 months of treatment, body movement function in the treatment group was more obvious, and the involvement of rehabilitation treatment could help to compensate the shortage of simple human umbilical cord mesenchymal stem cell transplantation. Cranial MRI and related biochemistry examinations have confirmed the efficiency and safety of human umbilical cord mesenchymal stem cell transplantation combined with rehabilitation treatment. This suggests that transplantation of human umbilical cord mesenchymal stem cells cultured in vitro for treatment of cerebral hemorrhage sequela has no special adverse reactions and it can acquire better effects in recovery of body movement function after combination with rehabilitation treatment. Related Articles | Metrics