Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2559-2562.doi: 10.3969/j.issn.1673-8225.2012.14.018

Previous Articles     Next Articles

Isolation, culture and identitication of Ovis aries amnion epithelial cells

Zhu Xue-min, Li Hai-jun, Mi Yan, Yang Yin-feng, Guan Hong-min, Cao Gui-fang   

  1. Laboratory of Animal Embryo and Developmental Biology, Inner Mongolia Agricultural University, Hohhot  010018, Inner Mongolia Autonomous Region, China
  • Received:2011-10-30 Revised:2011-12-01 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Cao Gui-fang, Doctor, Professor, Laboratory of Animal Embryo and Developmental Biology, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia Autonomous Region, China guifangcao@126.com
  • About author:Zhu Xue-min☆, Studying for doctorate, Lecturer, Laboratory of Animal Embryo and Developmental Biology, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia Autonomous Region, China zhuxuemin7195@126.com
  • Supported by:

    National High Technology Research and Development Planning (863 Program), No.2008AA101005*

PDF

362

Abstract:

BACKGROUND: The amnion epithelial cells (AECs) with stem cells properties have been successfully obtained after isolating, digesting and culturing human and rat amnion and AECs have the potential to differentiate into cells of the endoderm (liver, lung, epithelium), mesoderm (bone, fat), and ectoderm (neural cells),
OBJECTIVE: To obtain the Ovis aries AECs and detect the stem cell characteristics.
METHODS: Ovis aries amnion tissue was peeled off by mechanical way and the low velocity-trypLE (pancreatic enzyme replacement) digestion method was used to isolate and culture the Ovis aries amnion, and the Ovis aries AECs were obtained. RESULTS AND CONCLUSION: The immunofluorescence observation showed that the labeled protein of AECs such as Oct-4, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 could be expressed on Ovis aries AECs. RT-PCR experiments confirmed that the genes of Oct-4, Sox-2 and Rex-1 were expressed and Nanog was not expressed. The results indicate that the Ovis aries AECs with stem cells properties have been achieved successfully.

CLC Number: