Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2555-2558.doi: 10.3969/j.issn.1673-8225.2012.14.017

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Appropriate conditions for culture of umbilical cord blood mesenchymal stem cells  

Zhang Tao1, Li Min2, Xu Pei-ru2   

  1. 1Department of Urology, the Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China; 2Department of Pediatrics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-10-02 Revised:2011-11-14 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Xu Pei-ru, Chief physician, Professor, Doctoral supervisor, Department of Pediatrics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China xupeiru126@126.com
  • About author:Zhang Tao☆, Studying for doctorate, Attending physician, Department of Urology, the Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China

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Abstract:

BACKGROUND: There have been many experiments describing culture of umbilical cord blood mesenchymal stem cells (UCBMSCs) in terms of improvement of medium compositions, separation method of cord blood cells, and the time for changing liquid for the first time. A safe and efficient training system has not been established yet.
OBJECTIVE: To explore a better method for culture of UCBMSCs in vitro with different conditions.
METHODS: The umbilical cord blood of healthy parturiens was taken from the Department of Obstetrics of the First Affiliated Hospital of Xinjiang Medical University. Several factors which influence the primary culture process of UCBMSCs were analyzed include the time for changing liquid for the first time, different culture mediums and different levels of fetal bovine serum.
RESULTS AND CONCLUSION: Changing liquid at 5-7 days for the first time, low glucose DMEM or IMDE medium would be helpful to the growth of UCBMSCs. 30% fetal bovine serum and 20% fetal bovine serum did not produce different effects in culture of UCBMSCs.

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