Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2520-2523.doi: 10.3969/j.issn.1673-8225.2012.14.010

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Three kinds of two-dimensional gel electrophoresis sample preparation methods of rabbit bone marrow mesenchymal stem cells  

Qin Wan-an, Su Wei, Huang Zhao, Cui Xiang-rong, Guo Jie   

  1. Branch of Hand Surgery, Department of Traumatic Orthopedics, First Affiliated Hospital of Guangxi Medical University, Nanning  530021,Guangxi Zhuang Autonomous Region, China
  • Received:2011-09-19 Revised:2011-10-26 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Su Wei, Professor, Chief physician, Branch of Hand Surgery, Department of Traumatic Orthopedics, First Affiliated Hospital of Guangxi Medical University, Nanning 530021,Guangxi Zhuang Autonomous Region, China gxsuwei@163.com
  • About author:Qin Wan-an★, Master, Resident physician, Branch of Hand Surgery, Department of Traumatic Orthopedics, First Affiliated Hospital of Guangxi Medical University, Nanning 530021,Guangxi Zhuang Autonomous Region, China qinwanangx@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30860078*; Research Program of Education Department of Guangxi Zhuang Autonomous Region, No. 200710MS021*; Research Foundation of the First Affiliated Hospital of Guangxi Medical University*

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Abstract:

BACKGROUND: A variety of “standard” sample preparation methods for two-dimensional gel electrophoresis have been reported, but there is no single one can be universally applied to rabbit bone marrow mesenchymal stem cells (rBMSCs).
OBJECTIVE: To identif a suitable sample preparation method for rBMSCs two-dimensional gel electrophoresis (2-DE).
METHODS: After full protein extraction of rBMSCs cultured in vitro, protein samples were divided into three groups: untreated, acetone precipitation and 2-D clean-up kit group, the protein concentration was measured by Bradford method, electrophoresis maps were analyzed.
RESULTS AND CONCLUSION: Using 2-D clean-up kit to treat the protein sample could obtain the best electrophoresis map which showed lowest background, clearest protein spots, highest resolution and good reproducibility. Protein extraction treated with 2-D Clean-up was a more suitable 2-DE sample preparation method of rBMSCs.

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