Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2571-2575.doi: 10.3969/j.issn.1673-8225.2012.14.021

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Expression of tongue cancer stem cells marker CD44, ESA and CXCR4 in different environments

Huang Ying-hua1, Yao Jin-guang2, Kuang Xiao-cong3, Cai Jie4, Xie Ji-sheng5, Li Jun2, Chen Hai-bo2, Yang Yong-rong2   

  1. 1Department of Pathology, Guilin Medical School, Guilin  541002, Guangxi Zhuang Autonomous Region, China; 2Department of Stomatology; 5Department of Histology and Embryology, Youjiang Medical College, Baise  533000, Guangxi Zhuang Autonomous Region, China; 3Department of Pathophysiology;  4 Medical Research Center, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-09-25 Revised:2011-10-08 Online:2012-04-01 Published:2012-04-01
  • Contact: 课题负责人：姚金光，博士，教授，右江民族医学院口腔系，广西壮族自治区百色市 533000 yao7760698@126.com 并列通讯作者：邝晓聪，硕士，副教授，广西医科大学病理生理学教研室，广西壮族自治区南宁市 530021 bskxc@yahoo.com.cn
  • About author:Huang Ying-hua★, Master, Lecturer, Department of Pathology, Guilin Medical School, Guilin 541002, Guangxi Zhuang Autonomous Region, China hyh8150@gmail.com Yao Jin-guang☆, Doctor, Professor, Department of Stomatology, Youjiang Medical College, Baise 533000, Guangxi Zhuang Autonomous Region, China yao7760698@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30860326*

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Abstract:

BACKGROUND: Cancer stem cells marker expression in different environments differ greatly because the microenvironment can impact the expression of proteins.
OBJECTIVE: To discuss whether different cultures in vitro have influence on the expressions of cancer stem cells marker CD44, ESA and CXCR4.
METHODS: Tongue cancer stem cells TCA8113 cell line was obtained. In vitro, three different microenvironments were established: stem cell culture medium, conventional standard medium and conventional standard medium supplemented with adriamycin. The expressions of three proteins in TCA8113 tongue carcinoma cells were detected by immunohistochemistry (IHC) and flow cytometry (FCM).
RESULTS AND CONCLUSION: In all microenvironments, IHC showed the expressions of CD44 and ESA were “++”. In stem cell culture medium and the other microenvironments, the expressions of CXCR4 were “±” and “+” respectively. FCM found the expressions of CD44 and ESA were above 90% in all microenvironments; compared with conventional standard medium, the expression of CXCR4 was super low in stem cell culture medium, and recovery when tongue cancer cells reversed into conventional standard medium from stem cell culture medium; the expression of CXCR4 was increased in conventional standard medium supplemented with adriamycin. The expressions of cancer stem cell markers were various in different mircoenvironments; it needs to take various markers into account and combine with the experessions of markers on cancer cells in vivo to identify cancer stem cell markers. Different kinds of cancer stem cells may accumulate together by different microenvironments model.

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