Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2567-2580.doi: 10.3969/j.issn.1673-8225.2012.14.022

Previous Articles     Next Articles

Bone marrow mesenchymal stem cells transplantation for treatment of dilated cardiomyopathy in rats 

Xu Yan1, Zhang Yao2, Li Li-li2   

  1. 1Department of Emergency, Affiliated Hospital of Luzhou Medical College, Luzhou  646000, Sichuan Province, China; 2Department of Cardiology, Second Affiliated Hospital of Harbin Medical University, Harbin  150000, Heilongjiang Province, China
  • Received:2011-10-27 Revised:2012-01-11 Online:2012-04-01 Published:2012-04-01
  • About author:Xu Yan★, Master, Resident physician, Department of Emergency, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China xuyan_009@sina.com

PDF

305

Abstract:

BACKGROUND: Myocardial fibrosis induced by dilated cardiomyopathy (DCM) is the pathological basis of heart failure. At present, drug treatment, interventionaI therapy and surgical intervention cannot ameliorate necrotic myocardium and completely improve cardiac function．
OBJECTIVE: To investigate the effect of allogenic bone marrow mesenchymal stem cells (BMSCs) transplantation on cardiac function and myocardial fibrosis in rats with DCM.
METHODS: Forty Wistar rats were randomly divided into three groups: cell transplantation group (n=15), control group (n=15) and blank group (n=10). DCM models were established in cell transplantation group and control group. Four weeks after the models were established successfully, rats in cell transplantation group were injected with allogeneic BMSCs 150 μL (containing 3×106 cells). Rats in control group and blank group were also implanted with the culture medium in the same amount. 
RESULTS AND CONCLUSION: Compared with the blank group, echocardiography showed that the left ventricular end-systolic inside diameter was increased before transplantation, while the ejection fraction and fractional shortening was decreased significantly (P < 0.01) in cell transplantation group and control group. Four weeks after transplantation, the echocardiography showed that the left ventricular end-systolic inner diameter was decreased, while the ejection fraction and fractional shortening was increased obviously (P < 0.01) in cell transplantation group compared with before transplantation. Expression of cardiac collagen in cell transplantation group was lower than that in control group (P < 0.05). Compared with the control group, the expression of matrix metalloproteinase 2 and matrix metalloproteinase 9 was significantly increased in the other two groups (P < 0.05). In conclusion, bone marrow mesenchymal stem cells (BMSCs) transplantation can improve cardiac function and ameliorate myocardial fibrosis in rats with DCM.

CLC Number: