Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2491-2495.doi: 10.3969/j.issn.1673-8225.2012.14.005

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In vitro osteogenic induction of rat bone marrow mesenchymal stem cells  

Zhao Da-cheng, Wang Yu-liang, Dang Yue-xiu, Zhao Lin, Wang Jing, Wang Cui-fang   

  1. Second Hospital of Lanzhou University, Lanzhou   730030, Gansu Province, China
  • Received:2011-10-04 Revised:2012-03-01 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Wang Yu-liang, Master’s supervisor, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China wyl60918@sina.com
  • About author:Zhao Da-cheng★, Studying for master’s degree, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China zhaodacheng09@163.com
  • Supported by:

    the Natural Science Foundation of Gansu Province, No. 096RJZA077*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells are a kind of good seed cells. Bone marrow mesenchymal stem cells compounded onto biological scaffold for treatment of bone defects have acquired satisfactory outcomes and become a gradually increasing research area.
OBJECTIVE: To investigate the effect of in vitro osteogenic induction of rat bone marrow mesenchymal stem cells.
METHODS: Sprague-Dawley rat bone marrow mesenchymal stem cells were in vitro isolated by adherent method. Passage 3 well growing bone marrow mesenchymal stem cells were divided into two groups. The control group was cultured only with DMEM/F12 culture medium. The experimental group was cultured with DMEM/F12 culture medium containing osteogenic inductor.
RESULTS AND CONCLUSION: Primary bone marrow mesenchymal stem cells cultured by the whole bone marrow adherence method exhibited a shuttle-shaped or polygon-shaped appearance. After induced by osteogenic inductor, bone marrow mesenchymal stem cells adhered to plate wall in a circle or oval-shaped manner. Alkaline phosphatase activity was significantly higher in the experimental group than in the control group (P < 0.01). Calcified nodules appeared after alizarin red staining. Western blotting results showed that type Ⅰ collagen protein expression was significantly higher in the experimental group than in the control group (P < 0.01). ELISA quantitative analysis showed that osteocalcin was significantly higher in the experimental group than in the control group (P < 0.01). These findings suggest that whole bone marrow adherence method for culture of bone marrow mesenchymal stem cells is simple and practical and the cultured cells exhibit morphological and biological characteristics of osteoblasts after osteogenic induction.

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