Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2540-2544.doi: 10.3969/j.issn.1673-8225.2012.14.014

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Comparison of ex vivo culture characteristics between human and rabbit adipose derived stem cells   

Zhao Jian-hui1, Li Long2, Liang Li-hua3, Yi Cheng-gang1, Zeng Shu-hong1, Guo Shu-zhong1   

  1. 1Institute of Plastic Surgery, Xijing Hospital, the Fourth Military Medical University of Chinese PLA, Xi’an  710032, Shaanxi Province, China; 2Department of Oral and Maxillofacial Surgery; 3Department of Implantation, Stomatological Hospital of the Fourth Military Medical University of Chinese PLA, Xi’an  710032, Shaanxi Province, China
  • Received:2011-10-10 Revised:2011-11-02 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Guo Shu-zhong, Professor, Doctoral supervisor, Institute of Plastic Surgery, Xijing Hospital, the Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China shuzhong@fmmu.edu.cn
  • About author:Zhao Jian-hui★, Studying for master’s degree, Physician, Institute of Plastic Surgery, Xijing Hospital, the Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China zhaojianhui1985@163.com
  • Supported by:

    the National Natural Science Foundation of China, No.30973132*; Independent Innovation and Exploration Projects of the Subject Boost Plan of Xijing Hospital, No.XJZT08M10*; International Cooperation Projects of Research and Development Program of Shaanxi Province, No.2011KW-38*

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Abstract:

BACKGROUND: Whether there are differences of different sources of adipose derived stem/stromal cells (ASCs) when cultured in vitro has been poorly understood.
OBJECTIVE: To investigate the differences of ex vivo culture characteristics between human and rabbit ASCs under the same culture condition. 
METHODS: Human ASCs (hASCs) were isolated from intact fat of abdomen harvesting of skin grafts. Rabbit ASCs (rASCs) were derived from subcutaneous fat tissue of back. The hASCs and rASCs were cultured and passaged in vitro and the morphology of the cells was observed. Passage 3 ASCs were used to compare the ability of growth and proliferation, identification of CD surface molecules and the ability of adipogenic and osteogenic differentiation.
RESULTS AND CONCLUSION: Fibroblast-like adherent spindle-shaped cells could be isolated from both human and rabbit subcutaneous fat tissue. hASCs could passage after cultured for about 6-8 days, but rASCs were about 4-5 days. MTT results manifested that the growth climax of hASCs was the 4th day, whereas rASCs was the 6th day. Flow cytometry analysis showed the expression of CD29+CD31- could be seen both in human and rabbit ASCs. Both hASCs and rASCs had the cultural characteristics of stem cells. Compared with hASCs, rASCs had more powerful ability of proliferation and induction to fat whereas the ability of induction to bone was poor. Rabbit was a good choice of laboratory animal for fat transplantation research.

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