Abstract:

BACKGROUND: During in vitro tissue engineering construction, poor blood supply in transplanted areas or delayed revascularization of implants leads to nutrition deficiency of seed cells, metabolism disorders, proliferation, differentiation and secretion dysfunction or even death, and at last causes the transplantation failure.
OBJECTIVE: To investigate the feasibility of transfecting vascular endothelial growth factor 165 (VEGF165) gene into human adipose tissue-derived stem cells (ADSCs) for the neovascularization of tissue engineered adipose.
METHODS: ADSCs were transfected by adenovirus-mediated VEGF165 gene, and transfected ADSCs combined with collagen sponge were transplanted into the back of nude mice as experimental group. Non-transfected ADSCs with collagen sponge was used as cell group, and DMEM culture medium+collagen sponge as control group.
RESULTS AND CONCLUSION: Three months after transplantation, survival volume and capillary density of adipose tissue in the experimental group were higher than those of the cell and control groups, which in the cell group were higher than those of the control group (P < 0.01, P < 0.05). It is indicated that ADSCs can improve the neovascularization and survival volume of tissue-engineered adipose tissues and VEGF165 gene transfected ADSCs have a stronger ability of promoting the neovascularization of transplanted tissues.