Abstract:

BACKGROUND: Labeling of bone marrow mesenchymal stem cells (BMSCs) are important links to the research of differentiation and migration in vivo.
OBJECTIVE: To label the BMSCs with PKH26 and to explore the effect of PKH26 labeling on the biological activity, differentiation in vitro and tracking in vivo.
METHODS: BMSCs were isolated and cultivated from the bone marrow of rats. The passage 2 BMSCs were cultured and labeled with PKH26. The proliferation, cycle and apoptotic of cells in labeled group and unlabeled group were evaluated. The BMSCs in labeled group were performed with osteoblasts and lipoblasts induction in vitro. The PKH26-labeled BMSCs were transplanted through vein and the distribution of BMSCs in rat endometrium was observed under fluorescence microscope at 6 weeks after transplantation.
RESULTS AND CONCLUSION: The influence of PKH26-labelled cells on cell proliferation, apoptotic and cycle was not significant. And the labeled cells still had biological characteristics of osteoblasts and lipoblasts. In endometrium, PKH26-labelled cells were mainly distributed in epithelial cells and stromal cells. It suggested that the PKH26 labeling method could be used to study the homing, plasticity and transplantation of BMSCs.