Intra-articular injection of sodium hyaluronate versus placenta-derived mesenchymal stem cells-differentiated chondrocytes for treatment of knee osteoarthritis

doi:10.3969/j.issn.2095-4344.2014.50.018

Abstract

Abstract:

BACKGROUND: Placenta-derived mesenchymal stem cells have been shown to have a strong proliferative capacity, which can be induced to differentiate into nerve cells, vascular endothelial cells, epithelial cells and islet-like cells, but there are few studies about the chondrogenic differentiation in the treatment of knee osteoarthritis.
OBJECTIVE: To compare the intra-articular injection of sodium hyaluronate and placenta-derived mesenchymal stem cells-induced chondrocytes for treatment of knee osteoarthritis.
METHODS: (1) Knee osteoarthritis models were established in the left hind of New Zealand white rabbits. After
5.5 weeks of plaster fixation, the articular cartilage was observed under microscope. (2) Human placenta-derived mesenchymal stem cells were isolated and cultured using IV collagenase digestion, and flow cytometry was used to detect cell surface markers to identify the placenta-derived mesenchymal stem cells. Passage 3 placenta-derived mesenchymal stem cells were cultured in chondrocyte induction medium and observed under inverted microscope. (3) The rabbits were randomly divided into sodium hyaluronate group, placenta-derived mesenchymal stem cells group, and induced chondrocyte group, and then intra-articular injection of sodium hyaluronate, placenta-derived mesenchymal stem cells, and induced chondrocyte was given, respectively. After 1-5 weeks, the knee cartilage was taken and observed under microscope.
RESULTS AND CONCLUSION: In vitro cultured human placenta-derived mesenchymal stem cells proliferated to produce a large amount of adherent cells with typical mesenchymal cell morphology; after culture in inducing culture medium, cells presented with micelles and could not proliferate to form adherent cells. There were no adherent cells on the micelles base, showing human placenta-derived mesenchymal stem cells can differentiate into chondrocytes. Under hematoxylin-eosin staining, rabbit articular cartilage was repaired in all the groups, but the intra-articular injection of chondrocytes differentiated from placenta-derived mesenchymal stem cells were better than the intra-articular injection of sodium hyaluronate and placenta-derived mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, chondrocytes, osteoarthritis, knee

CLC Number:

R394.2

Li Zhi, Zhao Wei, Liu Wei, Zhou Ye, Jia Jing-qiao, Yang Li-feng. Intra-articular injection of sodium hyaluronate versus placenta-derived mesenchymal stem cells-differentiated chondrocytes for treatment of knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(50): 8140-8146.

Figures/Tables 4

References

[1] Frizziero A, Maffulli N, Masiero S, et al. Six-months pain relief and functional recovery after intra-articular injections with hyaluronic acid (mw 500-730 KDa) in trapeziometacarpal osteoarthritis. Muscles Ligaments Tendons J. 2014;4(2):256-261.
[2] Ishijima M, Nakamura T, Shimizu K, et al. Intra-articular hyaluronic acid injection versus oral non-steroidal anti-inflammatory drug for the treatment of knee osteoarthritis: a multi-center, randomized, open-label, non-inferiority trial. Arthritis Res Ther.2014;16(1):R18.
[3] Wexler SA, Donaldson C, Denning-Kendall P. Adult bone marrow is a rich source of human mesenchymal “stem” cells but umbilical cord and mobilized adult blood are not. Br J Haematol.2003;121(2):368-374.
[4] Rao MS, Mattson MP. Stem cells and aging: expanding the possibilities. Mech Ageing Dev.2001;122(7):713-734.
[5] Giuliani N, Mangoni M, Rizzoli V. Osteogenic differentiation of mesenchymal stem cells in multiple myeloma: identification of potential therapeutic targets. Exp Hematol.2009;37(8): 879-886.
[6] Banas A. Purification of adipose tissue mesenchymal stem cells and differentiation toward hepatic-like cells. Methods Mol Biol.2012;826:61-72.
[7] Sacchetti B, Funari A, Michienzi S, et al. Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment. Cell.2007;131(2):324-336.
[8] Aziz Aly LA, Menoufy HE, Ragae A, et al. Adipose stem cells as alternatives for bone marrow mesenchymal stem cells in oral ulcer healing. Int J Stem Cells.2012;5(2):104-114.
[9] Sun NZ, Ji H. In vitro differentiation of osteocytes and adipocytes from human placenta-derived cells. J Int Med Res.2012;40(2):761-767.
[10] Hayati AR, Nur Fariha MM, Tan GC, et al. Potential of human decidua stem cells for angiogenesis and neurogenesis. Arch Med Res.2011;42(4):291-300.
[11] Brooke G, Tong H, Levesque JP, et al. Molecular trafficking mechanisms of multipotent mesenchymal stem cells derived from human bone marrow and placenta. Stem Cells Dev.2008; 17(5):929-940.
[12] Abumaree MH, Al Jumah MA, Kalionis B, et al. Phenotypic and functional characterization of mesenchymal stem cells from chorionic villi of human term placenta. Stem Cell Rev. 2013;9(1):16-31.
[13] 费倩怡,刘春丽,朱振威,等.人胎盘来源间充质干细胞和大鼠骨髓间充质干细胞的生物学特征比较[J].吉林大学学报:医学版,2013, 39(3):467-471.
[14] 李治,赵伟,刘伟,等.胎盘来源间充质干细胞的体外诱导成软骨分化[J].中国组织工程研究,2014,18(32):5203-5208.
[15] Sakaguchi Y, Sekiya I, Yagishita K, et al. Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source. Arthritis Rheum.2005; 52(8):2521-2529.
[16] Dicker A, Le Blanc K, Aström G, et al. Functional studies of mesenchymal stem cells derived from adult human adipose tissue. Exp Cell Res.2005;308(2):283-290.
[17] Kadam S, Muthyala S, Nair P, et al. Human placenta-derived mesenchymal stem cells and islet-like cell clusters generated from these cells as a novel source for stem cell therapy in diabetes. Rev Diabet Stud.2010;7(2):168-182.
[18] Sabapathy V, Ravi S, Srivastava V, et al. Long-term cultured human term placenta-derived mesenchymal stem cells of maternal origin displays plasticity. Stem Cells Int.2012;2012: 174328.
[19] Lu GH, Zhang SZ, Chen Q, et al. Isolation and multipotent differentiation of human decidua basalis-derived mesenchymal stem cells. Nan Fang Yi Ke Da Xue Xue Bao.2011;1(2):262-265.
[20] Ringden O, Keating A. Mesenchymal stromal cells as treatment for chronic GVHD. Bone Marrow Transplant.2011;46(2):163-164.
[21] Jin J, Wang J, Huang J, et al. Transplantation of human placenta-derived mesenchymal stem cells in a silk fibroin/hydroxyapatite scaffold improves bone repair in rabbits. J Biosci Bioeng. 2014;118(5):593-598.
[22] 卢遥,王静成,顾加祥,等.兔胎盘来源间充质干细胞修复兔桡骨骨缺损的实验研究[J].中华创伤骨科杂志,2011,13(11):1060-1065.
[23] 钱寒光,苗宗宁,赵基栋,等.丝素蛋白/羟基磷灰石材料复合人胎盘间充质干细胞修复桡骨节段性骨缺损[J].中国组织工程研究与临床康复,2010,14(51):9507-9511.
[24] Richardson SM, Hoyland JA, Mobasheri R, et al. Mesenchymal stem cells in regenerative medicine: opportunities and challenges for articular cartilage and intervertebral disc tissue engineering. J Cell Physiol.2010; 222(1):23-32.
[25] Mobasheri A, Csaki C, Clutterbuck AL, et al. Mesenchymal stem cells in connective tissue engineering and regenerative medicine: applications in cartilage repair and osteoarthritis therapy. Histol Histopathol.2009;24(3):347-366.
[26] Onyekwelu I, Goldring MB, Hidaka C.Chondrogenesis, joint formation, and articular cartilage regeneration.J Cell Biochem. 2009;107(3):383-392.
[27] Shimomura K, Ando W, Tateishi K,et al.The influence of skeletal maturity on allogenic synovial mesenchymal stem cell-based repair of cartilage in a large animal model. Biomaterials. 2010;31(31):8004-8011