Biocompatibility of electrospun poly(lactide-co-glycolide)/polyethylene glycol nanofibrous scaffold with mouse neural stem cells

doi:10.3969/j.issn.2095-4344.2014.47.008

Abstract

Abstract:

BACKGROUND: Poly(lactide-co-glycolide) (PLGA) scaffold is widely used in tissue engineering, but its poor cell adhesion ability and strong hydrophobicity limit its further development and application.
OBJECTIVE: To study the biocompatibility of electrospun poly (lactide-co-glycolide)/polyethylene glycol (PLGA-PEG) nanofibrous scaffolds with mouse neural stem cells in vitro.
METHODS: Neural stem cells were isolated from embryos of CD-1 mice at 15 embryonic days. Electrospinning was used to prepare PLGA and PLGA-PEG nanofibrous scaffolds. Scanning electron microscope was used for scanning observation of scaffolds. The 5th passage neural stem cells were seeded onto PLGA and PLGA-PEG scaffolds respectively, and cultured in vitro.
RESULTS AND CONCLUSION: Interconnected porous network structure was observed in both two kinds of scaffolds under the scanning electron microscope. Fiber diameters and porosities of PLGA and PLGA-PEG scaffolds showed no significant differences (P > 0.05). Cell Counting Kit-8 detection showed neural stem cells grew well on both two kinds of scaffolds and the absorbance value of two groups increased continuously with incubation time (1, 3, 5, 7, 9, 11 days). And there were statistically significant differences in the absorbance values between two groups at each time point (P < 0.05). Moreover, the cell adhesion rate was significantly higher in the PLGA-PEG group than in the PLGA group at 3, 6, 9 hours of culture (P < 0.05). Hoechst 33342 staining showed normal morphology and quality of the nuclei, and significantly more cells were observed in the PLGA-PEG group than the PLGA group (P < 0.05). Under the scanning electron microscope, compared with the PLGA scaffold, the PLGA-PEG scaffold was better for growth and matrix secretion of neural stem cells. In conclusion, PLGA-PEG nanofibrous scaffolds prepared by electrospinning are safe, non-toxic and suitable for neural stem cells growth with well biocompatibility, appropriate aperture and porosity.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, neural stem cells, mice, cell proliferation

CLC Number:

R318

Liu Chang, Rong Li-min, Li Shang-fu, Pang Mao, Yang Yang, Liu Bin. Biocompatibility of electrospun poly(lactide-co-glycolide)/polyethylene glycol nanofibrous scaffold with mouse neural stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(47): 7585-7590.

Figures/Tables 2

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