Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (10): 1719-1723.doi: 10.3969/j.issn.2095-4344.2013.10.002

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In vitro differentiation of rat bone marrow mesenchymal stem cells into vascular smooth muscle cell-like cells

Yuan Wen-dan, Li Jing-min, Xu Fang   

  1. Basic College of Binzhou Medical University, Yantai 264003, Shandong Province, China
  • Received:2012-05-30 Revised:2012-06-27 Online:2013-03-05 Published:2013-03-05
  • About author:Yuan Wen-dan★, Master, Associate professor, Basic College of Binzhou Medical University, Yantai 264003, Shandong Province, China wendany@sohu.com

Abstract:

BACKGROUND: Studies have shown that the early activated adventitial fibroblasts cells are involved in the atherosclerosis and in the formation of vascular restenosis, and other studies have shown that some bone marrow mesenchymal stem cells are adherent, differentiate and participate in vascular remodeling. The experiment was to explore the possible mechanism of bone marrow mesenchymal stem cells involved in the atherosclerosis and the formation of vascular restenosis through analyzing the interaction between adventitial fibroblasts and bone marrow mesenchymal stem cells.
OBJECTIVE: To observe the morphological changes of bone marrow mesenchymal stem cells and the expression of vascular smooth muscle actin after bone marrow mesenchymal stem cells directly contacted with adventitial fibroblasts.
METHODS: Rat bone marrow msenchymal stem cells (DAPI-labeled nucleus) and adventitial fibroblasts were mixed and co-cultured for 7 days. Bone marrow mesenchymal stem cells were cultured alone as controls. The morphological changes of co-cultured cells were observed. The expression of vascular smooth muscle actin was detected with immunofluorescence staining.
RESULTS AND CONCLUSION: After co-cultured with adventitial fibroblasts, bone marrow mesenchymal stem cells exhibited DAPI-labeled blue nuclei and red stained cytoplasm (positive smooth muscle actin). With the prolongation of culture time, smooth muscle actin expression in the bone marrow mesenchymal stem cells was significantly increased. These results indicate that bone marrow mesenchymal stem cells can differentiate into vascular smooth muscle cell-like cells after directly contacting with adventitial fibroblasts.

Key words: stem cells, bone marrow-derived stem cells, smooth muscle actin, bone marrow mesenchymal stem cells, adventitial fibroblasts, co-culture, induced differentiation, provincial grants-supported paper, stem cell photographs-containing paper

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