Research about panaxtrial saponins on the relationship between cerebral ischemic tolerance and proliferation of endogenous neural stem cells

doi:10.3969/j.issn.2095-4344.2014.37.020

Abstract

Abstract:

BACKGROUND: Cerebral ischemia tolerance can promote proliferation of autologous neural stem cells in the hippocampus of cerebral infarction rats, but panaxtrial saponins effects on the proliferation of autologous neural stem cells in the brain have not been reported.
OBJECTIVE: To explore the relationship of panaxtrial saponins, ischemic preconditioning and proliferation of endogenous neural stem cells in the hippocampus of rats at 7 days after cerebral infarction, and to observe the effect on neurobehavioral scores of rats after cerebral infarction.
METHODS: Fifty Sprague-Dawley rats were included and randomly divided into five groups: sham group, ischemia group, ischemic control group, ischemic preconditioning group, and panaxtrial saponins group. In the latter four groups, acute models of cerebral infarction were established using Zea-Longa method. In the sham group, only an incision was made on the neck. The focal-focal ischemic tolerance models were established with twice suture method in the ischemic preconditioning and panaxtrial saponins groups. Sham operation was instead of ischemic preconditioning in the ischemic control group. In the panaxtrial saponins group, rats were given intraperitoneal injection of 100 mg/kg panaxtrial saponins at 7 days before modeling.
RESULTS AND CONCLUSION: After 7 days of cerebral infarction, the neurobehavioral score and the number of neural stem cells in the hippocampus were significantly increased in the ischemia group (P < 0.01); compared with the ischemia group, the neurobehavioral scores were lowered in the ischemic preconditioning and panaxtrial saponins groups (P < 0.01), while the number of neural stem cells in the hippocampus was increased (P < 0.01). However, there was no difference between the ischemic preconditioning and panaxtrial saponins groups (P > 0.05). In addition, differences in the neurobehavioral scores and the number of neural stem cells in the hippocampus were insignificant between the ischemic control group and ischemia group (P > 0.05). These findings indicate that panaxtrial saponins can play a role similar to ischemic tolerance, and thus improve neurologic impairment in rats with cerebral infarction.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: panax notoginseng, ginsenosides, brain ischemia, infarction, middle cerebral artery

CLC Number:

R394.2

Jiang Xiao-feng, Zhang Jie-wen, Luo Zu-ming. Research about panaxtrial saponins on the relationship between cerebral ischemic tolerance and proliferation of endogenous neural stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(37): 6014-6018.

Figures/Tables 1

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