Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (40): 5953-5958.doi: 10.3969/j.issn.2095-4344.2016.40.004

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Effects of electroacupuncture at points selected by orthogonal experiment on the extracellular signal regulated kinase signal pathway in a rat model of cerebral ischenia-reperfusion injury

Feng Yi-hui1, Zhu Zhi-hua1, Wu Chun-xiao2, Zhou Guo-ping2   

  1. 1Department of Acupuncture, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China; 2Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Revised:2016-08-25 Online:2016-09-30 Published:2016-09-30
  • Contact: Zhou Guo-ping, Chief physician, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • About author:Feng Yi-hui, M.D., Attending physician, Department of Acupuncture, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81173355

Abstract:

BACKGROUND: Acupuncture point matching is little reported in the electroacupuncture for cerebral ischemia-reperfusion injury.

OBJECTIVE: To explore the influence of electroacupuncture acupoints selected by orthogonal experiment on rats with cerebral ischemia reperfusion and extracellular signal regulated kinase (ERK) signal pathway.
METHODS: 150 Sprague-Dawley rats were randomly divided into five groups: sham operation group rats were exposed the middle cerebral artery only; models of cerebral ischemia reperfusion were established in the rats in model and orthogonal groups. Then rats in the orthogonal groups 1 (Baihui (DU 20), Quchi (LI 11), Zusanli (ST 36), and Chize (LU 5)), 2 (Baihui (DU 20), Zusanli (ST 36), Chize (LU 5), and Sanyinjiao (SP 6)) and 3 (Hegu (LI 4), Zusanli (ST 36), Chize (LU 5) and Sanyinjiao (SP 6)) were given electroacupuncture at acupoints selected by orthogonal experiment, respectively.
RESULTS AND CONCLUSION: Compared with the model group, in all orthogonal groups, the neurological function deficit scores were decreased, infarct volume was smaller, the number of microvessel density in the ischemia region and the p-ERK expression level in the cortex and hippocampal CA1 region of ischemic hemisphere were increased, and significant changes were found in the orthogonal group 3. These findings indicate that electroacupuncture at points of Hegu (LI 4), Zusanli (ST 36), Chize (LU 5) and Sanyinjiao (SP 6) selected by orthogonal experiment can significantly reduce the infarct volume of cerebral ischemia-reperfusion injury in rats possibly by regulating the ERK signaling pathway. 

中国组织工程研究杂志出版内容重点:肾移植肝移植移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植组织工程

Key words: Electroacupuncture, Brain Ischemia, Tissue Engineering

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