Exosomes derived from human umbilical cord blood mesenchymal stem cells: isolation, identification and biological characteristics

doi:10.3969/j.issn.2095-4344.2014.37.009

Abstract

Abstract:

BACKGROUND: Exosomes are membrane vesicles secreted by mesenchymal stem cells. Increasing studies have shown that mesenchymal stem cells can secrete exosomes via paracrine function to play a role in tissue injury. However, reports on how to isolate and identify exosomes derived from human umbilical cord blood mesenchymal stem cells are few.
OBJECTIVE: To extract, purify and identify exosomes derived from human umbilical cord blood mesenchymal stem cells.
METHODS: The cell culture supernatant of human umbilical cord blood mesenchymal stem cells was collected. Exosome was extracted and purified with ultrafiltration and gradient centrifugation methods. The morphology of exosome was observed by transmission electronic microscope, and the expressions of CD63, CD81, CD90, CD73, CD105, CD29, and CD166 in exosome of mesenchymal stem cells were analyzed by fluorescent activated cell sorting.
RESULTS AND CONCLUSION: Mesenchymal stem cells from human umbilical cord blood secreted exosome
which exhibited elliptic or saucer-like shape and its diameter ranged from 40 to 100 nm with membrane structure. Exosome could express the common surface adhesion molecules CD63, CD81 and the surface adhesion molecules CD90, CD73, CD105, CD29, CD166 of mesenchymal stem cells. These findings indicate that exosome may be secreted by mesenchymal stem cells of human umbilical cord blood, which contains plasma membrane proteins of umbilical cord blood mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: fetal blood, mesenchymal stem cells, exosomes, membrane proteins

CLC Number:

R394.2

Zhang Juan, Liu Feng, Zhang Wei, Cong Xu, Wang Cai-sheng, Wei Lai . Exosomes derived from human umbilical cord blood mesenchymal stem cells: isolation, identification and biological characteristics[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(37): 5955-5960.

Figures/Tables 3

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