Transforming growth factor-beta 1 expression in early enthesiopathy of Achilles tendon in rats after low-level laser therapy

doi:10.3969/j.issn.2095-4344.2014.24.007

Abstract

Abstract:

BACKGROUND: Low-level laser is a kind of therapeutic laser and has great potential application value in the field of sports medicine.
OBJECTIVE: To discuss the repairing mechanism of low-level laser therapy in early enthesiopathy of achilles tendon in rats through observing the change of transforming growth factor-β1 expression after low-level laser therapy.
METHODS: A total of 96 male Wistar rats aged 4 weeks were randomly divided into control group (n=8) and model group (n=88). Then model group was assigned randomly into two subgroups: model control group (n=48) and laser groups (n=40). The laser group was further allocated to 1, 2, 3, 7, 14 days of laser radiation, with eight rats in each. The rats of model group were trained by electrical stimulation for 5 weeks, and the rats of laser groups received laser radiation. The expression of transforming growth factor-β1 mRNA level in Achilles tendon was analyzed by real-time quantitative PCR. The transforming growth factor-β1 content was tested by double antibody ABC-ELISA.
RESULTS AND CONCLUSION: The transforming growth factor-β1 in model control group was increased significantly compared with control group immediately after modeling (P < 0.05). Then, transforming growth factor-β1 was gradually decreasing as the time prolonged. Compared with laser groups, transforming growth factor-β1 in model control group was decreased significantly at 7 and 14 days (P < 0.05). Low-level laser therapy promotes the early healing of achilles tendon enthesiopathy in rats, delays the decrease of transforming growth factor-β1 content, and maintains high level of transforming growth factor-β1 at 7-14 days. Low-level laser therapy plays an important role in regulating extracellular matrix.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: laser, Achilles tendon, tendinopathy, transforming growth factor beta, extracellular matrix

CLC Number:

R318

Shi Xiao-wei, Long Li-juan, Shen Yong-wei, Wang Zhi-peng. Transforming growth factor-beta 1 expression in early enthesiopathy of Achilles tendon in rats after low-level laser therapy[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(24): 3808-3813.

Figures/Tables 2

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