Impaired fracture healing and change of advanced glycation end products in vivo 
in type 2 diabetes rats

doi:10.3969/j.issn.2095-4344.2014.20.002

Abstract

Abstract:

BACKGROUND: Increasing attention has been paid on the role of advanced glycation end products in bone tissue. Glucose metabolic disorder is one of the main reasons for the increase of advanced glycation end products.
OBJECTIVE: To observe the change of advanced glycation end products expressed in type 2 diabetes rats, and to investigate the relationship between impaired fracture healing and change of advanced glycation end products expression in vivo.
METHODS: Thirty Sprague-Dawley rats were randomly and equally divided into two groups: control group (normal feeding) and experimental group (high fat and sucrosum diet feeding to establish type 2 diabetes model). After diabetes models were established, the model of distraction osteogenesis in the left tibiae of all the rats was produced. Distraction was given 0.3 mm per day and continued for 14 days.
RESULTS AND CONCLUSION: After the traction was complete, callus formation in distraction gap was obviously reduced in experimental group compared with control group by X-ray examination. The array of microcolumn formation was disordered and the area of primary matrix front was catachromasis by histology examination. The enzyme-linked immunosorbent assay results showed that, the level of advanced glycation end products was obviously elevated (P < 0.01) while osteocalcin was obviously reduced (P < 0.01) in experimental group in comparison with control group. The formation of distraction callus was impaired in the process of fracture healing and blood of type 2 diabetes rats. The increase of advanced glycation end products may be one of the reasons that cause impaired fracture healing in diabetic rats.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: diabetes mellitus, model, animals, fracture healing, tibial fracture, osteocalcin, advanced glycation end products

CLC Number:

R318

Liu Zhen-dong, Liu Ya-jiang, Gao Min-wei, Huang Zu-fa, Liao Xiao-jun, Shi Lei . Impaired fracture healing and change of advanced glycation end products in vivo
in type 2 diabetes rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(20): 3122-3126.

Figures/Tables 4

References

[1] Hui SL, Epstein S, Johnston CC Jr. A prospective study of bone mass in patients with type I diabetes. J Clin Endocrinol Metab.1985;60(1):74-80.
[2] Piepkorn B, Kann P, Forst T, et al. Bone mineral density and bone metabolism in diabetes mellitus. Horm Metab Res.1997; 29(11):584-591.
[3] Schwartz AV, Sellmeyer DE, Ensrud KE,et al.Older women with diabetes have an increased risk of fracture: a prospective study.J Clin Endocrinol Metab. 2001;86(1):32-38.
[4] Bouillon R, Bex M, Van Herck E, et al. Influence of age, sex and insulin on osteoblast function: osteoblast dysfunction in diabetes mellitus. J Clin Endocrinol Metab.1995;80(4): 1194-1202.
[5] Beam HA, Parsons JR, Lin SS.The effects of blood glucose control upon fracture healing in the BB Wistar rat with diabetes mellitus.J Orthop Res. 2002;20(6):1210-1216.
[6] 孔德娟,李恩,陈永春,等.晚期糖化终末产物对大鼠成骨细胞增殖和分化的影响[J].中国骨质疏松杂志,1999,5(3):29-32.
[7] 孔德娟.高级糖化终末产物与骨质疏松[J].国外医学:老年医学分册,1999,20(2):57-60.
[8] The Ministry of Science and Technology of the Peoples Republic of China. Guidance suggestion of caring laboratory animals. 2006-09-30.
[9] 李丁,苏海川,赵锦荣,等.骨组织总RNA的提取[J].第四军医大学学报,1999,20(12):1056.
[10] 茂欣,权金星,朱任之,等.一种从骨组织提取RNA的新方法的研究[J].兰州大学学报自然科学版,2000,36(5):22.
[11] 孙磊,王利群,韩一生,等.老年与青年人股骨头松质骨的比较[J].中国矫形外科杂志,1999,6(12):919-921.
[12] 李健,郭洪敏,聂志奎,等.2型糖尿病大鼠血清AGEs与骨代谢相关性分析[J].济宁医学院学报,2008,31(1):15-18.
[13] Liu Z, Aronson J, Wahl EC, et al. A novel rat model for the study of deficits in bone formation in type-2 diabetes. Acta Orthopaedica, 2007; 78(1):46-55.
[14] Spencer CP, Godsland IF, Cooper AJ,et al.Effects of oral and transdermal 17beta-estradiol with cyclical oral norethindrone acetate on insulin sensitivity, secretion, and elimination in postmenopausal women.Metabolism. 2000;49(6):742-747.
[15] Hein G, Weiss C, Lehmann G. Advanced glycation end product modification of bone proteins and bone remodelling: hypothesis and p reliminary immunohistochemical findings. Ann Rheum Dis.2006;65(1):101-104.
[16] Hofbauer LC, Brueck CC, Singh SK,et al.Osteoporosis in patients with diabetes mellitus.J Bone Miner Res. 2007;22(9): 1317-1328.