Icariin intervenes wear particles-induced osteolysis

doi:10.3969/j.issn.2095-4344.2014.17.001

Abstract

Abstract:

BACKGROUND: Osteolysis surrounding artificial joints is the main reason for the failure of joint loosening. Scholars all over the world are seeking an effective drug to inhibit osteolysis so as to reduce the occurrence of prosthetic loosening.
OBJECTIVE: To observe the effects of different doses of icariin on osteolysis induced by wear particles.
METHODS: The wear debris (Ti alloy and bone cement) was put into mouse cranium to create mouse models of calvarial osteolysis. Physiological saline was dropped in the cranium in the blank group. Different concentrations of icariin (30, 60 and 120 mg/kg) were given in the drug intervention group, by intragastric administration, once a day. At 2 weeks after model induction, mice were sacrificed. The structure of mouse cranium was observed under a microscope. Hematoxylin-eosin staining and immunohistochemical staining were performed. The number of osteoclasts and the changes in the calvarial osteolysis area of mice after intervention were calculated.
RESULTS AND CONCLUSION: Compared with the blank group, the number of absorption pits of bone slices that were stimulated by wear debris (Ti alloy and bone cement) and the number of osteoclasts were significantly increased, and absorption pits area was also bigger (P < 0.05). After intervention with icariin, osteolysis area and the number of osteoclasts became less (P < 0.05), especially in the 120 mg/kg gavage group, followed by 60 mg/kg and 30 mg/kg. Results suggested that Ti particles and bone cement particles can promote osteoclast proliferation and osteolysis. Icariin can suppress the formation of osteoclasts induced by particles, resulting in inhibiting osteolysis.

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

全文链接：

Key words: joint prosthesis, bone resorption, osteoclasts, titanium, polymethyl methacrylate, epimedium

CLC Number:

R318

Wang Jun, Zhu Xu-ri, Zhang Chao, Shen Ji-rong, Du Bin. Icariin intervenes wear particles-induced osteolysis[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(17): 2625-2631.

Figures/Tables 6

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