Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (10): 1547-1553.doi: 10.3969/j.issn.2095-4344.2014.10.011

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Effects of Corning® CellBIND® Surface medium on growth of human umbilical cord mesenchymal stem cells

Wan Ying1, Ben Liang1, Guan Zi-qiu 2, Li Chao1, Zhang Shi-dong1, Nie De-zhi1   

  1. 1 TuoHua Biological Technology Company, Siping 136000, Jilin Province, China
    2 Corning Incorporated, Shanghai 201206, China
  • Online:2014-03-05 Published:2014-03-05
  • Contact: Nie De-zhi, M.D., Technician in charge, TuoHua Biological Technology Company, Siping 136000, Jilin Province, China
  • About author:Wan Ying, M.D., TuoHua Biological Technology Company, Siping 136000, Jilin Province, China

Abstract:

BACKGROUND: Stem cells expansion technology in vitro is affected by the microenvironment of the culture system. So, to find an effective method is particularly important to promote cell adherent and growth.
OBJECTIVE: To compare the effects of different culture media on cell expansion.
METHODS: Human umbilical cord mesenchymal stem cells at a density of 5.0×104 cells/cm2 were inoculated into Corning® polystyrene culture dishes coated with or without poly-L-ornithine and Corning® CellBIND culture dishes. Cell adhesion and proliferation were observed, and expressions of cell adhesion proteins and cell markers were detected.
RESULTS AND CONCLUSION: Cell adhesion was promoted when cells were cultured in Corning® CellBIND® Surface medium coated with poly-L-ornithine for 24 hours, and the cultured cells grew at the logarithmic phase. The cell proliferation was also enhanced, and the cells expressed cell adhesion protein but not the cell markers of CD73, CD90, CD105. Corning® CellBIND® Surface medium was superior to Corning® polystyrene culture medium in the improvement of cell adhesion and proliferation. Additionally, both of these two media showed no influence on cell phenotype. These findings indicate that Corning® CellBIND® Surface medium can promote cell adhesion and proliferation, but shows no effects on cyclin and cell phenotype. This medium coated with poly-L-ornithine can further accelerate cell adhesion and proliferation, and stably express cell phenotype of human umbilical cord mesenchymal stem cells. 


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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Key words: mesenchymal stem cells, cell culture techniques, cell proliferation, ornithin

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