Carboxymethylated chitosan effects on proliferation of Schwann cells and expression of nuclear factor kappa B

doi:10.3969/j.issn.2095-4344.2014.03.010

Abstract

Abstract:

BACKGROUND: Carboxymethylated chitosan is shown to promote some kinds of cells proliferation, but its effects on proliferation of Schwann cells need further studies.
OBJECTIVE: To investigate the effects of carboxymethylated chitosan on proliferation of Schwann cells and expression of nuclear factor-κB in cultured Schwann cells.
METHODS: Schwann cells from Sprague-Dawley rats at logarithmic growth phase were seeded in 96-well plates, and cultured respectively with PBS, 0, 10, 50, 100, 200, 500, 1 000 mg/L carboxymethyl chitosan for 24 hours. Cell proliferation was detected using the cell counting kit-8 assay. After trypsin digestion, Schwann cells from Sprague-Dawley rats at logarithmic growth phase were used to prepare cell suspensions, which were seeded in 6-well cell culture plates and cultured respectively with 50, 100 and 200 mg/L carboxymethyl chitosan and PBS for 24 hours. Then, 5-bromo-2-deoxyuridine, real-time PCR and western blot assay were performed.
RESULTS AND CONCLUSION: Cell counting kit-8 and 5-bromo-2-deoxyuridine detection results showed that carboxymethyl chitosan at 50-1000 mg/L, especially at 200-500 mg/L, could promote Schwann cell proliferation. Real-time PCR and western blot results showed 50-200 mg/L carboxymethyl chitosan could promote nuclear factor κB mRNA and protein expression in Schwann cells in a dose-dependent manner, suggesting carboxymethyl chitosan can promote Schwann cell proliferation and expression of nuclear factor-κB in Schwann cells cultured in vitro.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, chitosan, cell proliferation, NF-kappa B

CLC Number:

R318

He Bin, Tao Hai-ying, Wei Ai-lin, Liu Shi-qing . Carboxymethylated chitosan effects on proliferation of Schwann cells and expression of nuclear factor kappa B[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(3): 389-394.

Figures/Tables 3

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