Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (25): 4731-4734.doi: 10.3969/j.issn.1673-8225.2011.25.042

Previous Articles     Next Articles

Experiment study of vessel wall reconstruction after a novel biological small-diameter vascular graft

Xu Yi-min1, Qi Song-tao2, Lin Lü-biao1, Zeng Shao-wen3, Li Wei-qiu4, Zhuang Bing-rong4   

  1. 1Department of Neurosurgery, the First Affiliated Hospital of Shantou University Medical College, Shantou  515041, Guangdong Province, China; 2Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou  510515, Guangdong Province, China; 3Department of Pathology, Central Hospital of Shantou City, Shantou  515041, Guangdong Province, China; 4Center Laboratory, Shantou University Medical College, Shantou  515041, Guangdong Province, China
  • Received:2011-03-11 Revised:2011-05-06 Online:2011-06-18 Published:2014-01-10
  • Contact: Qi Song-tao, Doctor, Professor, Chief physician, Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • About author:Xu Yi-min☆, Doctor, Associate chief physician, Department of Neurosurgery, the First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong Province, China xuym@163.com

PDF

596

Abstract:

BACKGROUND: Compared with human vessels, vascular prosthesis has the greastest advantage for its rich source. Besides, its characteristics are similar to the human vessel, and vascular prosthesis has high histocompatibility after graft.
OBJECTIVE: To observe the survival and life status, graft histocompatibility and histopathological changes of the reconstructed vascular wall in experimental dogs within eighteen months following the implantation of biological small-diameter vascular graft.
METHODS: By crosslinking fixation, removing antigen, covalent bonding with heparin and coupling with specific polypetide capable of sticking and enriching growth factors, a kind of highly anticoagulant biological artificial vessel with the diameter of 3.5 to 4.5 mm was prepared. Artificial vascular grafts were implanted end-to-end by a continuous suture in the common carotid artery of the experimental dogs. Sample procedure was performed at different time for postoperative eighteen months, for histopathological examination.
RESULTS AND CONCLUSION: Only a little adhesion between the artificial artery and the surounding tissue was found during sampling procedure. Eight weeks after grafting, the host tissue penetrated the poros of grafts and protruded into the lumen, forming the neointima of the graft vessel. Twelve weeks after grafting, endothelial cells were sparsely distributed throughout the neointima near the stoma. Six months after grafting, endothelial cells were present on the whole surface of the patent graft lumen. Twelve months after grafting, VG staining of the graft wall revealed that the graft stent had been replaced by a large amount of newly grown collagen fibers and blood capillaries. The previous graft stent was partly replaced by the host vascular tissue. Eighteen months after grafting, the previous graft stent was mostly replaced by the host vascular tissue. All of the above proves that the novel biological small-diameter vascular graft has early and intact formation of the neointima with strong capacity of vessel wall reconstruction and intravascular stent regeneration, taken together with the good biocompatibility and stability.

CLC Number: