Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (25): 4647-4650.doi: 10.3969/j.issn.1673-8225.2011.25.021

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Effects of polymethyl methacrylate particles on human synoviocytes receptor activator of nuclear factor kappa B ligand / osteoprotegerin expression

Xiong Ao1, Su Zhi-xiong2, Ma Ze-tao1, Kang Bin1, Xin Feng1   

  1. 1Department of Orthopedics, Shenzhen Hospital of Peking University, Shenzhen  518036, Guangdong Province, China
    2Department of Orthopedics, First Affiliated Hospital of Tsinghua University, Beijing  100016, China
  • Received:2010-12-29 Revised:2011-02-15 Online:2011-06-18 Published:2014-01-10
  • About author:Xiong Ao, Attending physician, Department of Orthopedics, Shenzhen Hospital of Peking University, Shenzhen 518036, Guangdong Province, China xiongao@21cn.com

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Abstract:

BACKGROUND: Various factors can influence bone metabolism through expression of osteoprotegerin/receptor activator of nuclear factor-κB/ligand of receptor-activator of nuclear factor-κB (OPG/RANKL/RANK) system. Whether bone cement particles also affects bone metabolic balance around prosthesis via OPG/RANKL/RANK expression in aseptic loosening remains poorly understood.
OBJECTIVE: To investigate the effects of polymethyl methacrylate (PMMA) particles on the expression of RANKL/OPG in human synoviocytes.
METHODS: In vitro, human synovial cells were cultured with different concentrations of PMMA particles (0, 0.2, 1 and 10 g/L). The expression of RANKL, OPG and their ratio of each group was detected by fluorescence quantitative PCR at different time points.
RESULTS AND CONCLUSION: Compared with the control group, the expression of RANKL and OPG in each experimental group was significantly down-regulated with time prolonged. While, there was no significant difference in the ratios of RANKL/OPG expression. Synoviocytes biologically reacting to PMMA particles had no interference on the dynamic balance of bone metabolism around prosthesis, thus play no obvious role in the aseptic loosening of artificial prosthesis.

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