Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (25): 4611-4614.doi: 10.3969/j.issn.1673-8225.2011.25.013

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Preparation of recombinant human bone morphogenetic protein 2 loaded chitosan nanosphere and its cytotoxicity in vitro

Wang Wei1,2, Yin Qing-shui1, Zhang Yu1   

  1. 1Orthopedic Hospital of Guangzhou General Hospital of Guangzhou Military Command, Guangzhou  510010, Guangdong Province, China
    2Postgraduate College, Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Received:2011-03-16 Revised:2011-05-10 Online:2011-06-18 Published:2014-01-10
  • Contact: Yin Qing-shui, Master, Professor, Chief physician, Postgraduate College, Southern Medical University, Guangzhou 510515, Guangdong Province, China gk_yqs@126.com
  • About author:Wang Wei☆, Doctor, Attending physician, Orthopedic Hospital of Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, Guangdong Province, China; Postgraduate College, Southern Medical University, Guangzhou 510515, Guangdong Province, China guagualang@sina.com
  • Supported by:

    Military Medical Research during the Eleventh Five-year Plan, No. 06G045*

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Abstract:

BACKGROUND: The study on bone morphogenetic protein (BMP) release approach has more important significance, but in recent years studies focus on the release effect of microspheres loaded various bone growth factors. However, the biocompatibility, especially cytotoxicity of drug-loaded chitosan nanoparticles, has not been reported.
OBJECTIVE: To prepare and characterize a controlled release system for recombinant human bone morphogenetic protein 2 (rhBMP-2) and to evaluate the cytotoxicity and biological safety of chitosan nanospheres as a carrier for controlled release of rhBMP-2.
METHODS: The rhBMP-2 loaded chitosan nanospheres were prepared using an ionic crosslinking method with ripolyphosphate  as the crosslinking agent. Transmission electron microscope was used to evaluate the morphological properties and particle size analyzer was used to analysis particle size distribution. A cytotoxic test in vitro was adopted to determine the biological safety of rhBMP-2 loaded chitosan nanospheres.
RESULTS AND CONCLUSION: The rhBMP-2 loaded chitosan nanospheres were spherical in shape, and showed a smooth surface. The mean diameter of nanospheres was 230 nm. The cell relative growth rates (RGR) of rhBMP-2 loaded chitosan nanospheres and blank chitosan nanospheres groups were 0 or 1, and all were qualified. The rhBMP-2 loaded chitosan nanospheres prepared by ionic crosslinking method show a good property and biocompatibility. Such a novel type of controlled release system has the potential of being applied for the bone tissue engineering.

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