Effect of small interfering RNA on gene expression of synovial cells in patients with rheumatoid arthritis

doi:10.3969/j.issn.2095-4344.2013.46.014

Abstract

Abstract:

BACKGROUND: The etiological factor for rheumatoid arthritis remains unclear, but the effects of nuclear factor-κB on the onset of rheumatoid arthritis have been gradually paid great attention by rheumatologists.
OBJECTIVE: By using the RNA interference (RNAi) technique to block the signal pathway of nuclear factor-κB mRNA of human rheumatoid arthritis synovial cells, this study explored its application prospect in the treatment of rheumatoid arthritis.
METHODS: The synovial cells were isolated, digested, and cultured for further use. In accordance with the design principle of small interfering RNA (siRNA), target sequences of siRNA of nuclear factor-κB were identified, and siRNA expression vector of nuclear factor-κB was synthesized and constructed. The four pGenesil-1/nuclear factor-κB siRNA expression vectors were transfected into the first passage of synovial cells that well grew. Blank and negative control groups were set. Cells at 12, 24, 48, 72 hours, 5 and 7 days after transfection were collected, and RNA was extracted. Intracellular nuclear factor-κB mRNA expression levels were measured, and siRNA plasmid vector that could effectively inhibit nuclear factor-κB mRNA expression was screened out.
RESULTS AND CONCLUSION: Nuclear factor-κB highly expressed in synovial cells after human rheumatoid arthritis. 3#pGenesil-1/nuclear factor-κB apparently suppressed nuclear factor-κB mRNA expression in synovial cells with human rheumatoid arthritis. RNAi technique blocked nuclear factor-κB mRNA expression. Therefore, the block of nuclear factor-κB signal pathway might be a good target for rheumatoid arthritis gene therapy.

Key words: arthritis, osteoarthritis, arthritis, rheumatoid, rheumatoid factor, rheumatoid nodule, nuclear factor-kappa B, synovial membrane

CLC Number:

R318

Hou Chun-feng, Sun Min, Li Shu-jie, Zhao Jian-hong, Wang Ji-bo. Effect of small interfering RNA on gene expression of synovial cells in patients with rheumatoid arthritis[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(46): 8062-8068.

Figures/Tables 3

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