Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8390-8393.doi: 10.3969/j.issn.2095-4344.2012.45.006

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Bone marrow mesenchymal stem cells migrate to the zone of spinal cord injury

Jia Quan-zhang1, Li Dong-jun2, Chen Yu-bing3, Sun Jing-hai1, Wu Chuan-zhen1, Wang Feng-hua1, Xu Shuang1, Liu Li-ping3, Jiang Da-wei2, Gao De-xuan2   

  1. 1The 208 Hospital of People’s Liberation Army, Changchun 130062, Jilin Province, China
    2Department of Medicine, Yanbian University, Yanji 133002, Jilin Province, China
    3the Second Affiliated Hospital of Jilin University, Changchun 130062, Jilin Province, China
  • Received:2012-05-04 Revised:2012-09-23 Online:2012-11-04 Published:2012-11-04
  • About author:Jia Quan-zhang☆, Doctor, Chief physician, the 208 Hospital of People’s Liberation Army, Changchun 130062, Jilin Province, China jqz208@sohu.com

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Abstract:

BACKGROUND: How to observe the survival and prognosis of bone marrow mesenchymal stem cells after migration to the spinal cord injury zone remains controversial.
OBJECTIVE: To observe the migration situation of bone marrow mesenchymal stem cells in the rat spinal cord injury zone.
METHODS: Thirty-six Wistar rats were randomly divided into two groups. In the experimental group, the rats were used to make the spinal cord injury model, and after 1 week, 1 mL DAPI labeled bone marrow mesenchymal stem cells (1×109/L) were daily transplanted into the rats through the tail vein for 2 days. The rats in the control group were not subjected to spinal cord injury induction, but they received bone marrow mesenchymal stem cells transplantation at the same time with the same method. The frozen sections of injured spinal cord were prepared at 5, 10 and 15 days after transplantation, and the migration of bone marrow mesenchymal stem cells was observed under the confocal laser scanning microscope.
RESULTS AND CONCLUSION: At 5 days after the transplantation in the experimental group, the fluorescence marked bone marrow mesenchymal stem cells were observed in the vessels of spinal cord injury tissue; 10 days after transplantation, bone marrow mesenchymal stem cells were dispersed in extravascular tissue; 15 days after transplantation, the fluorescence marked bone marrow mesenchymal stem cells were widely diffused in the injury tissue. While, the DAPI labeled bone marrow mesenchymal stem cells were not observed in the control group. Results show that bone marrow mesenchymal stem cells can migrate to the injured spinal tissue through spinal cord-blood barrier after transplantation through the tail vein.

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