Effect of bone marrow mesenchymal stem cells combined with Danhong injection on expression of GAP-43 and Bcl-2 after cerebral infarction

doi:10.3969/j.issn.2095-4344.2013.32.019

Abstract

Abstract:

BACKGROUND: Danhong injection, scavenging free radicals and inhibiting lipid peroxidation, can improve microenvironment injury after cerebral infarction.
OBJECTIVE: To explore the influence of bone marrow mesenchymal stem cells combined with Danhong injection on expression of GAP-43 and Bcl-2 after cerebral infarction in rats.
METHODS: Sixty Wistar rats were selected to prepare models of cerebral infarction by middle cerebral artery occlusion and then randomly divided into control group, bone marrow mesenchymal stem cell group, and combination group. Control group received tail vein injection of PBS. Bone marrow mesenchymal stem cell group received tail vein injection of 2.5×10⁹/L bone marrow mesenchymal stem cell suspension. Combination group received injection of 2.5× 10⁹/L bone marrow mesenchymal stem cell suspension+2 mL/kg Danhong injection, for 5 consecutive days, once a day.
RESULTS AND CONCLUSION: There were no significant differences in the neurological dysfunction scores among the three groups at 24 hour and 3 days after implantation (P > 0.05). The neurological dysfunction scores in the ombination group were significantly lower than those in the bone marrow mesenchymal stem cell group and control group at 1 and 2 weeks after transplantation (P < 0.05). In the combination group, GAP-43 and Bcl-2 expression was significantly higher than the bone marrow mesenchymal stem cell group and control group (P < 0.05). Bone marrow mesenchymal stem cell transplantation combined with Danhong injection can significantly promote the local expression of GAP-43 and Bcl-2 after cerebral infarction, and has obvious inhibitory effects on cell apoptosis in rats with cerebral infarction.

Key words: stem cells, stem cells and traditional Chinese medicine, bone marrow mesenchymal stem cells, cerebral infarction, Danhong injection, GAP-43, Bcl-2

CLC Number:

R394.2

Li Jin-yan, Zhang Zhe-cheng. Effect of bone marrow mesenchymal stem cells combined with Danhong injection on expression of GAP-43 and Bcl-2 after cerebral infarction [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(32): 5871-5876.

Figures/Tables 6

References

[1] Li J, Zhu H, Liu Y, et al. Human mesenchymal stem cell transplantation protects against cerebral ischemic injury and upregulates interleukin-10 expression in Macacafascicularis. Brain Res. 2010;1334:65-72.

[2] 张洪连,吴晓牧. 骨髓间充质干细胞移植治疗脑梗死及存在问题[J].中华脑血管病杂志,2010,4(4):300-307.

[3] 张婷勇.骨髓间充质干细胞移植并用单唾液酸神经节苷脂治疗大鼠脑梗死[J]. 中国组织工程研究与临床康复,2011,15(14): 2557-2561.

[4] 朱路文,刘宏光,张春艳.任务导向性训练对局灶性脑梗死大鼠前肢运动功能及缺血区突触素和生长相关蛋白-43表达的影响[J].中国康复理论与实践,2012,18(4):319-323.

[5] 刘志婷,俞天虹,曲铁兵,等.补阳还五汤对小鼠局灶性脑缺血后生长相关蛋白43和突触素表达的影响[J].中华行为医学与脑科学杂志,2012,21(12):1070-1072.

[6] 龚誉华,程光宇,程为平.中药补脑止痫散对癫痫大鼠海马神经元凋亡基因Bax和Bcl-2表达的影响[J].环球中医药,2012,5(2): 105-107.

[7] Jiang W, Xia F, Han J, et al. Patterns of Nogo-A, NgR, and RhoA expression in the brain tissues of rats with focal cerebral infarction. Transl Res. 2009;154(1):40-48.

[8] Kim SS, Yoo SW, Park TS, et al. Neural induction with neurogenin1 increases the therapeutic effects of mesenchymal stem cells in the ischemic brain. Stem Cells. 2008;26(9):2217-2228.

[9] Theus MH, Wei L, Cui L, et al. In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain. Exp Neurol. 2008;210(2):656-670.

[10] Shen LH, Li Y, Gao Q, et al. wn-regulation of neurocan expression in reactive astrocytes promotes axonal regeneration and facilitates the neurorestorative effects of bone marrow stromal cells in the ischemic rat brain. Glia. 2008;56(16):1747-1754.

[11] Kim SS, Yoo SW, Park TS, et al. Neural induction with neurogenin1 increases the therapeutic effects of mesenchymal stem cells in the ischemic brain. Stem Cells. 2008;26(9):2217-2228.

[12] Li Y, Chen J, Chen XG, et al. Human marrow stromal cell therapyfor troke in rat: Neurotrophins and functional recovery. Neurology. 2002;59(4):514-523.

[13] Zhang J, Li Y, Zhang ZG, et al. Bone marrow stromal cells increase oligodendrogenesis after stroke. J Cereb Blood Flow Metab. 2009;29(6):1166-1174.

[14] 杨锁成,汪坤,张振凌.HPLC同时测定复方丹参降浊丸中丹参酮ⅡA和丹酚酸B[J].中国实验方剂学杂志,2011,17(9):84-86.

[15] 韩丽妹,付艳,张丽红,等.新型药用辅料对复方丹参片药剂学性质及溶出性能的影响[J].复旦学报:医学版,2011,38(3):233-236.

[16] Freret T, Bouet V, Leconte C,et al. Behavioral deficits after distal focal cerebral ischemia in mice: Usefulness of adhesive removal test. Behav Neurosci. 2009;123(1):224-230.

[17] 王溯.丹红治疗对急性脑梗死大鼠运动功能改善的影响论著[J]. 临床和实验医学杂志, 2010,9(16):1204-1205.

[18] 张春燕.红花注射液对急性脑梗死病人疗效、血脂、血液流变学的影响[J].中国新药与临床杂志,2001,20(4):273-275.

[19] 舒榕,刘冬玲,王强.瑞芬太尼对大鼠脑缺血再灌注损伤后大脑皮质bcl-2和Caspase-3表达的影响[J].华中科技大学学报:医学版, 2012,41(1):72-75.

[20] 陈文杰,胡伟鑫.依达拉奉对颅脑损伤大鼠脑组织Bcl-2和Bax表达的影响[J].北方药学,2012,9(5):44-45.

[21] 杨金龙,程朝辉,刘双,等.2-(2-苯并呋喃基)-2-咪唑啉对大鼠脑缺血再灌注损伤后Bcl-2和Bax表达的影响[J].温州医学院学报, 2012, 42(2):129-131.

[22] 董银凤,陈前芬.大豆异黄酮对大鼠脑缺血再灌后ATP酶及bcl-2和bax的影响[J].蚌埠医学院学报,2012,37(1):8-11.

[23] 马晓丽,张丽华,张士岭,等.EP对HIBD新生大鼠脑组织BCL-2及BAX表达的影响[J].中国伤残医学,2012,20(1):24-25.

[24] 张博,胡婷婷,张萍,等.EPO对大鼠急性脊髓损伤后Bcl-2及Bax表达与运动神经元凋亡的影响[J].辽宁中医药大学学报,2012, (10): 122-123.

[25] 张迪,裴少保,王海峰,等.海水浸泡对兔脊髓损伤Bcl-2和Bax基因表达的影响[J].中华全科医学,2012(2):168-169,333.

[26] 宋月平,苏立凯,钟娅莉,等.替勃龙对大鼠神经细胞Bcl-2、Bax表达的影响[J].河北医药,2012,34(1):14-16.

[27] 税林,王霞,金茂强.大鼠脑干损伤GAP-43、NOS3、HIF-1α的表达及其表达时相的相关性研究[J].新疆医科大学学报,2012, 35(4): 457-461,466.

[28] 王瑞红,侯世科,吴志鸿.GAP-43与受损后视网膜神经节细胞存活和再生关系的研究进展[J].国际眼科纵览,2012,36(3): 172-177.

[29] 张玉辉,郝飞,朴花,等.ATP诱导大鼠原代培养海马神经元神经毒性及对GAP-43表达的影响[J].神经解剖学杂志,2011,27(5): 527-533.

[30] 郑里翔,汪敏,乔玉丹,等.补骨脂汤对痴呆大鼠海马神经元保护及GAP-43基因表达的影响[J].时珍国医国药,2011,22(10): 2382-2384.