Chinese Journal of Tissue Engineering Research ›› 2026, Vol. 30 ›› Issue (7): 1736-1746.doi: 10.12307/2026.061
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Pan Dong1, Yang Jialing1, Tian Wei2, Wang Dongji2, Zhu Zheng3, Ma Wenchao3, Liu Na3, Fu Changxi3
Received:
2025-01-25
Revised:
2025-05-13
Accepted:
2025-06-20
Online:
2026-03-08
Published:
2025-08-19
Contact:
Fu Changxi, PhD, Associate professor, Department of Physical Education, Xuzhou University of Technology, Xuzhou 221008, Jiangsu Province, China
About author:
Pan Dong, PhD, Lecturer, School of Physical Education, Lianyungang Normal College, Lianyungang 222006, Jiangsu Province, China
Supported by:
CLC Number:
Pan Dong, Yang Jialing, Tian Wei, Wang Dongji, Zhu Zheng, Ma Wenchao, Liu Na, Fu Changxi. Resistance exercise activates skeletal muscle satellite cells in aged rats: role of adiponectin receptor 1 pathway[J]. Chinese Journal of Tissue Engineering Research, 2026, 30(7): 1736-1746.
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2.8 各组大鼠骨骼肌卫星细胞数量比较 免疫荧光染色显示,配对盒基因7阳性细胞呈绿色荧光、成肌分化因子阳性细胞呈红色荧光,DAPI阳性呈蓝色荧光,见图6A。与青年安静组比较,衰老安静组配对盒基因7阳性/成肌分化因子阳性细胞率降低(P < 0.05)。与衰老安静组比较,衰老运动组配对盒基因7阳性/成肌分化因子阳性细胞率升高(P < 0.05)。与衰老运动组比较,衰老运动抑制剂组配对盒基因7阳性/成肌分化因子阳性细胞率降低(P < 0.05),见图6B。 2.9 各组大鼠骨骼肌蛋白表达量比较 与青年安静组比较,衰老安静组总蛋白含量以及脂联素受体1、过氧化物酶体增殖物激活受体γ辅激活子1α、核呼吸因子1、线粒体转录因子A、磷酸化蛋白激酶B/蛋白激酶B、磷酸化哺乳动物雷帕霉素靶蛋白/哺乳动物雷帕霉素靶蛋白、磷酸化P70核糖体蛋白S6激酶/P70核糖体蛋白S6激酶、配对盒基因7、成肌分化因子、肌细胞生成素、生肌因子5蛋白表达量下调(P < 0.05)。与衰老安静组比较,衰老运动组总蛋白含量以及脂联素受体1、过氧化物酶体增殖物激活受体γ辅激活子1α、核呼吸因子1、线粒体转录因子A、磷酸化蛋白激酶B/蛋白激酶B、磷酸化哺乳动物雷帕霉素靶蛋白/哺乳动物雷帕霉素靶蛋白、磷酸化P70核糖体蛋白S6激酶/P70核糖体蛋白S6激酶、配对盒基因7、成肌分化因子、肌细胞生成素、生肌因子5蛋白表达量上调(P < 0.05)。与衰老运动组比较,衰老运动抑制剂组总蛋白含量以及脂联素受体1、过氧化物酶体增殖物激活受体γ辅激活子1α、核呼吸因子1、线粒体转录因子A、磷酸化蛋白激酶B/蛋白激酶B、磷酸化哺乳动物雷帕霉素靶蛋白/哺乳动物雷帕霉素靶蛋白、磷酸化P70核糖体蛋白S6激酶/P70核糖体蛋白S6激酶、配对盒基因7、成肌分化因子、肌细胞生成素、生肌因子5蛋白表达量下调(P < 0.05),见图7。 "
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