Astragalus injection strengthens biological viability of rat neural stem cells

doi:10.3969/j.issn.2095-4344.2013.27.017

Abstract

Abstract:

BACKGROUND: Neuroscience and brain science researches have paid attention to the effect of astragalus membranaceus in the treatment of neurologic impairment disease and neural regeneration. Studying astragalus membranaceus effects on neural stem cells are becoming a new research direction.
OBJECTIVE: To explore the effects of astragalus injection on biological viability of rat neural stem cells.
METHODS: Neural stem cells of Wistar rats were separated and cultured. Immunofluorescence staining was applied to identify the neural stem cells. The purified cells were gained by the second subcultivation in vitro, and then the cells were randomly divided into control group and astragalus injection groups with various concentrations (50, 200, 400 g/L) to culture for 6, 12 and 24 hours. The activity of cells was tested by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, and then the immunohistochemistry was applied to detect the expressions of neuron-specific enolase and glial fibrillary acidic protein in the 50 g/L astragalus injection group after induced for 7 days.
RESULTS AND CONCLUSION: The viability of neural stem cells increased significantly after intervention with different concentrations of astragalus injection for 6 hours as compared with the control group (P < 0.05). However, there was no difference in the cell viability after treated with different concentrations of astragalus injection for 24 hours (P > 0.05). Compared with the control group, the cells in the 50 g/L astragalus group differentiated rapidly, and the number of positive cells for neuron-specific enolase was increased significantly (P < 0.05). The neural stem cells proliferation was hastened, and its differentiation was promoted by the interference of astragalus injection.

Key words: stem cells, stem cells and traditional Chinese medicine, basic experiments of stem cells, traditional Chinese medicine, astragalus injection, neural stem cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, biological activity, cell differentiation, stem cell photographs-containing paper

CLC Number:

R394.2

Zhang Li1, Luo Xiu-cheng, Yang Shi-zhao, Zhang Jun-feng, Yang Ji-ping, Zhao Zhao-hua, Zhang Hui . Astragalus injection strengthens biological viability of rat neural stem cells[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.27.017.

Figures/Tables 4

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