Chinese Journal of Tissue Engineering Research
Cai Rong-hui, Liu Kang
Received:
2012-10-09
Revised:
2012-11-20
Online:
2013-07-02
Published:
2013-07-02
About author:
Cai Rong-hui★, Master, Associate chief physician, Zhongshan People’s Hospital, Zhongshan 528400, Guangdong Province, China
caironghui88@163.com
CLC Number:
Cai Rong-hui, Liu Kang. Tissue-engineered tendon construction using bone marrow mesenchymal stem cells induced by bone morphogenetic protein 12[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.27.001.
流式细胞仪检测细胞表面标记CD29,CD31,CD34,CD44,结果显示无论是实验组(DMEM+骨形态发生蛋白12),还是对照组(DMEM),其CD44和CD29阳性标记均出现单峰,CD31和CD34表达阴性,证明所培养的骨髓间充质干细胞是均一的细胞群,不含造血细胞、内皮细胞等其他细胞。应用SPSS 16.0软件,对实验组和对照组各CD值进行统计学分析,P均 > 0.05。 从上述分析结果看,实验组与对照组CD29值统计学分析,P > 0.05,证明两组差异无显著性意义。CD31,CD44,CD34统计学分析,P 也> 0.05,这结果说明骨形态发生蛋白12不会改变骨髓间充质干细胞表面标志,只会使骨髓间充质干细胞增殖分化加速。 2.4 组织化工程肌腱镜下形态学、力学及组织病理学观察结果 见图6-13及表2。 体外构建肌腱、Ⅰ型胶原-聚羟基乙酸缝线、丝线缝合方法修复家兔肌腱损伤的形态学结果:"
应用SPSS 16.0软件分别对实验组1、实验组2、实验组3结果进行配对t 检验,其中实验组1、实验组3左、右侧相比,P < 0.05;实验组2左、右侧相比,P > 0.05。 从上述结果可以看出,实验组1中,骨形态发生蛋白12+聚羟基乙酸重建肌腱的力学强度明显优于Ⅰ型胶原-聚羟基乙酸组;而实验组2中骨形态发生蛋白12+聚羟基乙酸重建肌腱的力学强度与丝线缝合组差异无显著性意义,这可能与丝线刺激纤维肉芽组织增生,使断端接触面积增大,从而增强了肌腱抗牵拉能力有关;至于实验组3中骨形态发生蛋白12+聚羟基乙酸重建肌腱的力学强度与正常肌腱强度差异存在显著性意义,说明组织工程重建肌腱还有待进一步改进,以增强抗牵拉力,达到完美修复肌腱损伤的程度。 体外构建肌腱、Ⅰ型胶原-聚羟基乙酸缝线、丝线缝合方法修复家兔肌腱12周时组织病理学结果:"
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