Tissue-engineered tendon construction using bone marrow mesenchymal stem cells induced by bone morphogenetic protein 12

doi:10.3969/j.issn.2095-4344.2013.27.001

Abstract

Abstract:

BACKGROUND: The previous methods for the repair of tendon defect include end-to-end method, autologous tendon graft, tendon allograft or artificial tendon transplantation, but each method has its advantages and disadvantages.
OBJECTIVE: To investigate the feasibility of constructing tissue-engineered tendon by rabbit bone marrow mesenchymal stem cells as seed cells which were induced by bone morphogenetic protein 12 and with collagen-polyglycolic acid composite as frameworks in the repair of rabbit tendon defects.
METHODS: Bone marrow was separated from rabbit proximal femur to harvest cells, and the cells were passaged to the second generation and induced with 10 μg/L bone morphogenetic protein 12. Then the passage 2 cells were implanted into the prefabricated tissue-engineered tendon on the polyglycolic acid stitch with certain percentage together with collagenⅠsolution. The rabbits were used to establish the Achilles tendon defect models, and different methods were used to repair Achilles tendon defect: tissue-engineered tendon, collagenⅠ- polyglycolic acid stitch and end-to-end suturing in silk. Morphology, mechanics and shistopathology of the tissue-engineered tendon were observed.
RESULTS AND CONCLUSION: Pathomorphological observation of histological section after 12 weeks showed that multiple fusiform fibroblasts were homogeneously distributed in collagen in the direction of stress mechanics. Fibrocytes increased obviously, new small vessels could be seen and collagen was found aligned compactedly. In collagen Ⅰ-polyglycolic acid stitch group, a part of fibroplasia hyperplasia accompanied by granulation tissue formation could be seen, the collage fibers were in loose filamentous network and the cells were distributed disorderly and unevenly. Granulation tissue formed around the fibrous tissue in the silk group. Biomechanics strength in bone morphogenetic protein 12+polyglycolic acid group was better than that in the collagen Ⅰ-polyglycolic acid group, and there was significant difference when compared with suture silk group. The biomechanics strength of the bone morphogenetic protein 12+polyglycolic acid group was lower than that of normal tendon. It is possible to construct a tissue-engineered tendon with autologous bone marrow mesenchymal stem cells as seed cells induced by bone morphogenetic protein 12 and with the collagen-polyglycolic acid as the framework. Constructed tissue-engineered tendon has biomechanics characteristics and can be used to repair Achilles tendon defect.

Key words: stem cells, bone marrow-derived stem cells, bone morphogenetic protein, induction, bone marrow mesenchymal stem cells, collagen, polyglycolic acid, tendon, defect, tissue engineering, biomechanics, repair, stem cell photographs-containing paper

CLC Number:

R394.2

Cai Rong-hui, Liu Kang. Tissue-engineered tendon construction using bone marrow mesenchymal stem cells induced by bone morphogenetic protein 12[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.27.001.

Figures/Tables 7

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