Improved laminectomy for constructing a rat model of spinal cord injury

doi:10.12307/2023.825

Abstract

Abstract: BACKGROUND: Spinal cord injury has become a worldwide problem. Experimental animal modeling is the first step to explore the disease; however, there is a lack of experimental animal models that are more effective.
OBJECTIVE: To establish a rat model of spinal cord injury that can be replicated, regulated, has little trauma, low mortality, low blood loss, wide application and short intraoperative time.
METHODS: Forty Sprague-Dawley rats with similar body mass were selected and randomly divided into conventional model group and improved model group (n=20 per group), where the model of spinal cord injury was made using Allen’s method and improved method, respectively. In the improved model group, a dental drill was used for biting. Operation time, intraoperative blood loss, mortality, and Basso-Beattie-Bresnahan locomotor rating scale scores at 1, 3, 6, 9, 12, and 15 days after beating were compared between the two groups.
RESULTS AND CONCLUSION: The improved method for constructing the animal model of secondary spinal cord injury has the advantages of shorter modeling time, less intraoperative blood loss, lower mortablity, and more centralized Basso-Beattie-Bresnahan score data as compared with the conventional method, which proves that the improved method is more suitable for establishing the model of secondary spinal cord injury.

Key words: spinal cord injury, animal model, Allen’s method, improved, rat, motor function, Basso-Beattie-Bresnahan locomotor rating scale

CLC Number:

Du Kairan, Deng Qiang, Guo Tiefeng, Zhang Yanjun, Peng Randong, Li Junjie, Wang Yurong, Zhang Kaidong, Luo Linzhao. Improved laminectomy for constructing a rat model of spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(32): 5173-5177.

Figures/Tables 4

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