Characterization and photothermal effect of indocyanine green encapsulated poly lactic acid-co-glycolic acid microspheres

Abstract

Abstract: BACKGROUND: Indocyanine green, an efficient photothermal conversion agent, can be used for photothermal therapy of oral squamous cell carcinoma, but it has some shortcomings such as water instability and photodegradation. Using carrier to encapsulate indocyanine green to improve its stability was of great significance for exploring photothermal therapy of oral squamous cell carcinoma.
Objective: Indocyanine green encapsulated poly lactic acid-co-glycolic acid microspheres were fabricated to delay the photodegradation of indocyanine green and improve its photothermal stability to develop reagents for photothermal treatment of oral squamous cell carcinoma.
Methods: The microspheres were prepared by emulsion-solvent evaporation method. The morphology, particle size distribution, surface charge, drug loading and encapsulation efficiency were characterized. The photothermal effect of free indocyanine green and microspheres were investigated at different concentrations, four laser on/off cycles, and left exposed to light for 0, 3, 6 and 9 days. Morphological changes of SCC-25 cells after different treatments were observed under light microscope, and in vitro photothermal anti-tumor effect of microspheres were evaluated by CCK8.
Results: The microspheres had smooth surfaces with particle sizes of (2.54±0.29)μm, zeta potential of -(20.2±1.58)mV, encapsulation efficiency and loading efficiency were (69.24±1.29) % and (4.87±0.15) %, respectively. Compared with free indocyanine green, microspheres have more stable photothermal properties, and tumor cells shrink to spherical shape after photothermal, which can effectively reduce the viability of SCC-25 cells.
Conclusion: Indocyanine green encapsulated poly lactic acid-co-glycolic acid microspheres possessed efficient photothermal conversion ability and can obviously delay photobleaching and photodegradation of indocyanine green. Poly lactic acid-co-glycolic acid is a suitable vehicle of indocyanine green and can be used for photothermal therapy of oral squamous cell carcinoma.

Key words: poly lactic acid-co-glycolic acid, indocyanine green, microsphere, oral squamous cell carcinoma , photothermal therapy, SCC-25 cell, near-infrared, emulsion-solvent evaporation method

CLC Number:

FAN Yaru, LI Ruixin , LI Fengji, LUO Rui, LIU Hao, YAN Yingbin. Characterization and photothermal effect of indocyanine green encapsulated poly lactic acid-co-glycolic acid microspheres[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(在线): 1-6.

Figures/Tables 4

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