Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (5): 724-729.doi: 10.12307/2022.118
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Li Weiming, Xu Qingwen, Li Yijun, Sun Yanbo, Cui Jin, Xu Pengyuan
Received:
2020-09-30
Revised:
2020-10-13
Accepted:
2021-01-19
Online:
2022-02-18
Published:
2021-10-28
Contact:
Li Weiming, Second Ward of Department of Gastrointestinal Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China
About author:
Li Weiming, MD, Associate chief physician, Second Ward of Department of Gastrointestinal Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China
Supported by:
CLC Number:
Li Weiming, Xu Qingwen, Li Yijun, Sun Yanbo, Cui Jin, Xu Pengyuan . Deep seawater promotes wound healing in diabetic mice by activating PI3K/Akt pathway[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(5): 724-729.
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2.4 深层海水对糖尿病小鼠创面肉芽新生血管形成的影响 各组小鼠创面苏木精-伊红染色结果,见图2A。对照组和自来水组小鼠创伤后第3天创面具有大量炎性细胞浸润及少量纤维母细胞,第10天可见肉芽组织及毛细血管和纤维母细胞;深层海水组小鼠创伤后第3天就可观察到纤维母细胞、毛细血管和少量炎性细胞,第10天可见新生肉芽组织,有较多胶原纤维和少量毛细血管。 此外Western blot检测结果显示,深层海水组小鼠创伤后第3,7,10天创面组织中的血管内皮生长因子和碱性成纤维细胞生长因子蛋白表达水平明显高于对照组和自来水组(P < 0.01),自来水组小鼠创面组织中的血管内皮生长因子和碱性成纤维细胞生长因子蛋白表达水平与对照组比较差异无显著性意义(P > 0.05),见图2B。结果表明,深层海水会促进创面新生肉芽的形成,进一步说明深层海水会促进创面愈合。"
2.6 深层海水通过调控PI3K/Akt信号通路对糖尿病小鼠创面愈合的影响 基于深层海水处理糖尿病小鼠后对PI3K/Akt信号通路的影响,进一步探讨深层海水是否通过激活PI3K/Akt信号通路促进小鼠创面愈合。 2.6.1 创面苏木精-伊红染色 对照组小鼠创伤后第3天创面有大量炎症细胞浸润和纤维母细胞;第10天有少量炎性细胞、大量胶原纤维并有新生表皮。深层海水组小鼠创伤后第3天创面只有少量炎性细胞浸润,并有少量胶原纤维及毛细血管;第10天有较多的胶原纤维和毛细血管,同时伴有新生表皮的生成。深层海水+LY294002组小鼠创面苏木精-伊红染色结果与对照组相似。各组不同时间点创面组织形态观察见图4。"
2.6.3 RT-PCR与Western blot检测结果 qRT-PCR及Western blot检测结果显示见图5,6。深层海水组小鼠各时间点创面组织中胶原Ⅰ、胶原Ⅲ和转化生长因子β1的蛋白及mRNA表达高于对照组(P < 0.05,P < 0.01),且在第7天达到最高值;白细胞介素1β和肿瘤坏死因子α的蛋白及mRNA表达低于对照组(P < 0.05,P < 0.01),且在第7天达到最低值。深层海水+LY294002组胶原Ⅰ、胶原Ⅲ、转化生长因子β1、白细胞介素1β和肿瘤坏死因子α的蛋白及mRNA表达与对照组比较差异均无显著性意义(P > 0.05),说明LY294002可逆转深层海水对胶原、促炎因子和促愈合因子表达的调控作用。由此可知,深层海水通过激活PI3K/Akt通路促进了创面组织中胶原和促愈合因子的表达,从而加速糖尿病小鼠创伤创面愈合。"
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