Gastrodin interferes with motor function recovery and growth associated protein-43 expression in a rabbit model of spinal cord injury

doi:10.12307/2021.160

Abstract

Abstract: BACKGROUND: Studies have reported that gastrodin has anti-inflammatory, antioxidant and neuroprotective effects in the treatment of spinal cord injury.
OBJECTIVE: To study the effect of gastrodin on the recovery of motor function and the expression of growth associated protein-43 (GAP-43) in animals with spinal cord injury.
METHODS: Thirty New Zealand white rabbits were divided into sham operation group, control group and experimental group, with 10 rabbits in each group. The control and experimental groups used modified Allen’s percussion method to establish a spinal cord injury model. The experimental group was injected with 100 mg/(kg•d) gastrodin intravenously. The sham operation and control groups were injected with 10 mL of normal saline intravenously for 7 continuous days. At 1, 3, 5, and 7 days after modeling, the rats in each group were scored for neurological function and neuroelectrophysiological testing; at 7 days after modeling, the injured spinal cord tissue was taken and stained with hematoxylin-eosin and Nissl, and used for western blot detection, immunofluorescence observation. The experimental protocol was approved by the Animal Experiment Ethics Committee of Guilin Medical University, with an approval No. GMC201805018.
RESULTS AND CONCLUSION: The neurological function scores of the hind limbs were significantly higher in the experimental group than the control group at different time points after modeling (P < 0.05). Compared with the sham operation group, the latency period of motor evoked potential seizures at different time points after modeling in the control group became longer (P < 0.05), and the peak-to-peak amplitude decreased (P < 0.05). Compared with the control group, in the experimental group, the motor evoked potential latency was shortened (P < 0.05), and the peak-to-peak amplitude increased (P < 0.05) at 3, 5, and 7 days after modeling. Hematoxylin-eosin staining showed that in the control group, syringomyelia was seen, the structure was incomplete, the boundary between gray matter and white matter was unclear, and neurons in the gray matter were necrotic and apoptotic, while the experimental group showed cavitation and necrosis, intact spinal cord tissue, and relatively clear gray matter nodes. Nissl staining showed that there were no neurons but a large amount of glial cells proliferated in the control group; in the experimental group, neurons survived and glial cells proliferated relatively less. Western blot detection and immunofluorescence observation showed that the GAP-43 protein expression was highest in the experimental group, higher in the control group and lowest in the sham operation group. To conclude, gastrodin can promote the recovery of motor function in rabbits after spinal cord injury, and may promote axonal regeneration in the injured spinal cord by increasing the expression of GAP-43 protein.

Key words: gastrodin, spinal cord injury, nerve injury and repair, growth associated protein-43, neurological function, experimental animal

CLC Number:

Zhang Heng, Zhang Xianping, Dai Houjie, Huang Changzhao, Wang Ruiying. Gastrodin interferes with motor function recovery and growth associated protein-43 expression in a rabbit model of spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(29): 4626-4631.

Figures/Tables 6

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