Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (25): 3981-3987.doi: 10.12307/2021.008
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Gao Shan1, Huang Dongjing1, Hong Haiman1, Jia Jingqiao2, Meng Fei1
Received:
2020-06-17
Revised:
2020-06-24
Accepted:
2020-08-04
Online:
2021-09-08
Published:
2021-03-24
Contact:
Meng Fei, Master’s supervisor, Professor, Department of Obstetrics, Affiliated Central Hospital of Shenyang Medical College, Shenyang 110024, Liaoning Province, China
About author:
Gao Shan, Master, Associate chief physician, Department of Obstetrics, Affiliated Central Hospital of Shenyang Medical College, Shenyang 110024, Liaoning Province, China
Supported by:
CLC Number:
Gao Shan, Huang Dongjing, Hong Haiman, Jia Jingqiao, Meng Fei. Comparison on the curative effect of human placenta-derived mesenchymal stem cells and induced islet-like cells in gestational diabetes mellitus rats[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(25): 3981-3987.
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2.1 诱导前人胎盘间充质干细胞形态学特征 胎盘组织在培养瓶中培养至14 d时,可见细长短小梭形细胞,待80%细胞融合后进行传代培养至第3代,大多数细胞表现为长梭形,排列整齐,贴壁均匀,细胞增殖活力良好,可进行下一步鉴定。 2.2 人胎盘间充质干细胞鉴定结果 流式细胞仪检测第3代人胎盘间充质干细胞阴性表达CD14-FITC(0.18%)、CD34-PE (0.30%)、HLA-DR-APC (0.03%),阳性表达CD44-FITC (99.35%)、CD90-PE(99.02%)、CD105-APC(99.76%),见图1,上述结果与胎盘干细胞表面标志相符[23]。第3代人胎盘间充质干细胞成骨诱导21 d经茜素红染色呈阳性,有大小不等橘红色钙结节;成脂肪诱导21 d经油红O染色呈阳性,细胞为红色,核为蓝色,脂肪颗粒呈红色;成软骨诱导21 d经甲苯胺蓝染色呈阳性,细胞呈蓝色,局部呈球状,见图2。"
2.7 各组大鼠体质量变化 各组大鼠妊娠后体质量均随时间增加,但不同处理组增加程度不同,见表1。 妊娠第6天:各妊娠期糖尿病组大鼠体质量均低于正常孕鼠组,组间总体差异有显著性意义(F=41.19,P=0.000 1)。LSD法两两比较显示,未干预组和生理盐水干预组大鼠体质量显著低于正常孕鼠组(P < 0.05),人胎盘间充质干细胞干预组和胰岛样细胞干预组体质量显著高于未干预组和生理盐水干预组(P < 0.05),人胎盘间充质干细胞干预组、胰岛样细胞干预组和正常孕鼠组间差异无显著性意义,未干预组、生理盐水干预组间差异无显著性意义。 妊娠第12天:各妊娠期糖尿病组大鼠体质量均低于正常孕鼠组,组间总体差异有显著性意义(F=41.19,P=0.001)。LSD法两两比较显示,未干预组和生理盐水干预组大鼠体质量显著低于正常孕鼠组(P < 0.05),人胎盘间充质干细胞干预组和胰岛样细胞干预组体质量显著高于未干预组和生理盐水干预组(P < 0.05),人胎盘间充质干细胞干预组体质量显著低于正常孕鼠组(P < 0.05),胰岛样细胞干预组体质量显著高于人胎盘间充质干细胞干预组(P < 0.05),但正常孕鼠组、胰岛样细胞干预组间差异无显著性意义,未干预组、生理盐水干预组间差异无显著性意义。 妊娠第18天:各组间总体差异有显著性意义(F=125.67,P=0.000 1)。LSD法两两比较显示,未干预组和生理盐水干预组大鼠体质量显著低于正常孕鼠组(P < 0.05),人胎盘间充质干细胞干预组和胰岛样细胞干预组体质量显著高于未干预组和生理盐水干预组(P < 0.05),人胎盘间充质干细胞干预组体质量显著低于正常孕鼠组(P < 0.05),胰岛样细胞干预组体质量显著高于人胎盘间充质干细胞干预组(P < 0.05),但正常孕鼠组、胰岛样细胞干预组间差异无显著性意义,未干预组、生理盐水干预组间差异无显著性意义。"
2.8 各组大鼠空腹血糖水平 注射人胎盘间充质干细胞和胰岛样细胞后,妊娠期糖尿病大鼠空腹血糖均随时间延长而逐渐降低,见表2。 妊娠第6天:各妊娠期糖尿病组大鼠空腹血糖均高于正常孕鼠组,总体差异有显著性意义(F=29.47,P=0.000 1),组间两两比较结果表明,人胎盘间充质干细胞干预组和胰岛样细胞干预组大鼠空腹血糖分别显著低于未干预组和生理盐水干预组(P < 0.05),但人胎盘间充质干细胞干预组、胰岛样细胞干预组间差异无显著性意义,未干预组、生理盐水干预组间差异也无显著性意义。妊娠第12天:各妊娠期糖尿病组大鼠空腹血糖显著高于正常孕鼠组,总体差异有显著性意义(F=47.13,P=0.000 1),组间两两比较结果表明,人胎盘间充质干细胞干预组和胰岛样细胞干预组血糖分别显著低于未干预组和生理盐水干预组(P < 0.05),人胎盘间充质干细胞干预组、胰岛样细胞干预组血糖均较妊娠第6天有所降低,且胰岛样细胞干预组血糖显著低于人胎盘间充质干细胞干预组(P < 0.05)。妊娠第18天:各妊娠期糖尿病组大鼠空腹血糖仍显著高于正常孕鼠组,总体差异有显著性意义(F=66.36,P=0.000 1),组间两两比较结果表明,人胎盘间充质干细胞干预组和胰岛样细胞干预组血糖显著低于未干预组和生理盐水干预组(P < 0.05),人胎盘间充质干细胞干预组、胰岛样细胞干预组血糖均较妊娠第12天有所降低,且胰岛样细胞干预组血糖依旧显著低于人胎盘间充质干细胞干预组 (P < 0.05)。"
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