Relationship between root resorption and intrusive force during maxillary molar intrusion in Beagle dogs

doi:10.3969/j.issn.2095-4344.3143

Abstract

Abstract: BACKGROUND: Root resorption is a common complication in the process of tooth intrusion. The choice of intrusion force and the development of root resorption are issues that clinicians are more confused and concerned about.
OBJECTIVE: To investigate the effect of different force values on root absorption during molar intrusion in Beagle dogs.
METHODS: In this study, the Beagle dog was used as the research object to establish the animal model of experimental root resorption. Through morphology, hematoxylin-eosin staining, immunohistochemistry and western blot, the root surface absorption and the expression of osteoprotegerin (OPG)/receptor activator for nuclear factor kappa B ligand (RANKL) in periodontal tissue were observed.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining results showed that root absorption was limited to alveolar bone and cementum at a intrusive force of 150-200 g. In the 250 g group, dentin absorption also occurred around the root tip. Immunohistochemical results showed similar RANKL/OPG ratios in the control group and the 150 g group, and the RANKL/OPG ratio in the 200 g group and the 250 g group presented an increasing trend. Pairwise comparison between the two groups showed statistically significant differences except for the control group and the 150 g group as well as the control group and the 200 g group (P < 0.05). Western blot results showed that RANKL/OPG ratio showed an increasing trend during 3-14 days, and after 14 days, the ratio went down. Pairwise comparison between groups showed that there were statistically significant differences among all the groups except for the 3 days and 7 days groups (P < 0.05). To conclude, with the increase of the additive force, the degree of root absorption will be aggravated. Excessive intrusive force will result in irreversible root absorption. A 150 g force is the recommended intrusive force for molar intrusion. RANKL/OPG ratio can be used as a biological indicator for the early diagnosis of root absorption.

Key words: molar intrusion, planting nail, root absorption, osteoprotegerin, animal model, dog

CLC Number:

Liu Hong, Wan Zhe, Zhang Zhen, Zhang Qin. Relationship between root resorption and intrusive force during maxillary molar intrusion in Beagle dogs[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(14): 2172-2176.

Figures/Tables 5

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