Application of digital subtraction angiography in dog hindlimb
arteriography

doi:10.3969/j.issn.2095-4344.2402

Abstract

Abstract:

BACKGROUND: The research on lower extremity vascular disease is becoming popular. In the experiment of large animals as research platform, it is necessary to detect the shape and density of lower extremity vascular in order to study and verify the effectiveness of intervention measures, and this detection method needs high feasibility and repeatability.

OBJECTIVE: To investigate the feasibility, influencing factors and application value of digital subtraction angiography in lower limb arteriography of a canine animal model.

METHODS: Six Beagle dogs were fixed on the working bed after anesthesia and punctured percutaneously through the femoral artery with an indwelling needle. Contrast agent was injected artificially. Digital subtraction angiography bolus chase technology was used to collect contrast images. The image effect and influencing factors were analyzed.

RESULTS AND CONCLUSION: (1) The final arteriography images of all six Beagle dogs’ hind limbs were clear, and the branches of small vessels could be distinguished without artifacts. (2) The first beagle shook when encountering contrast agent stimulation during the initial angiography, resulting in poor image effect. After fixing the limbs and diluting the contrast agent, the clear images were obtained by re-angiography. (3) These results indicate that arteriography using digital subtraction angiography can clearly reveal the shape and density of blood vessels in canine animal models. It is simple and reproducible, and is of great significance for research on animal models of limb ischemia.

Key words: digital subtraction angiography, animal model, dogs, big animals, lower limb ischemia, shape of blood vessels, density of blood vessels

CLC Number:

Zhang Shiyong, Wang Chengwei, Wang Xue, Yan Xiangli, Wang Jie. Application of digital subtraction angiography in dog hindlimb arteriography[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(5): 704-708.

Figures/Tables 1

References

[1] 肖婷,王爱红,许樟荣,等. 436例糖尿病足截肢相关因素分析[J].中华内分泌代谢杂志,2009,25(6):591-594.
[2] ERDEM M, BOSTAN B, GÜNEŞ T, et al. Protective effects of melatonin on ischemia-reperfusion injury of skeletal muscle. Eklem Hastalik Cerrahisi. 2010;21(3):166-171.
[3] PHELPS EA, LANDÁZURI N, THULÉ PM, et al. Bioartificial matrices for therapeutic vascularization. Proc Natl Acad Sci U S A. 2010;107(8):3323-3328.
[4] 王华,王伯胤. 糖尿病足下肢动脉病变影像学诊断研究进展[J]. 中国全科医学,2010,13(23):2543-2546.
[5] 米晓云,石保新,张壮志,等. 细粒棘球绦虫幼虫——原头蚴感染比格犬(Beagle)试验[J].中国兽医学报,2010,30(9):1223-1224.
[6] 宋洁,曹燕,黄茜,等.国内比格犬的生物学指标研究及相关应用进展[J].医学动物防制,2012,27(6):650-652.
[7] 侯明明,于维良,孙振林.新型犬脊柱侧弯动物模型的建立[J].临床骨科杂志,2006,9(2):170-172.
[8] 李鸿帅,张长青,白跃宏,等.固定一侧前肢后对犬后肢负重的影响[J].中国修复重建外科杂志,2008,22(1):66-69.
[9] KIM BJ, PIAO Y, WUFUER M. Biocompatibility and efficiency of biodegradable magnesium-based plates and screws in the facial fracture model of beagles. J Oral Maxillofac Surg. 2018; 76(5):1055-1064.
[10] NAKAMURA T, HARA Y, TAGAWA M, et al. Recombinant human basic fibroblast growth factor accelerates fracture healing by enhancing callus remodeling in experimental dog tibial fracture. J Bone Miner Res.1998;13(6):942-949.
[11] OMOTO O, YASUNAGA Y, ADACHI N, et al. Histological and biomechanical study of impacted cancellous allografts with cement in the femur: a canine model. Arch Orthop Trauma Surg.2008;128(12):1357-1364.
[12] FENG S, ZUOQIN Y, CHANGAN G, et al. Prediction of traumatic avascular necrosis of the femoral head by single photon emission computerized tomography and computerized tomography: an experimental study in dogs. Chin J Traumatol. 2011;14(4):227-232.
[13] 黄文华,蒋国民,张贤舜,等. DSA步进技术在下肢动脉疾病诊断中的应用[J].实用医学杂志, 2012, 28(4):622-623.
[14] POMPOSELLI F. Arterial imaging in patients with lower extremity ischemia and diabetes mellitus. J Vasc Surg. 2010; 100(5):412-423.
[15] 裴小燕,杜彦李,李双. CTA､MRA与DSA在脑血管狭窄疾病诊断中的应用[J].临床合理用药杂志,2016,9(15):174-175.
[16] TOEPKER M, MAHABADI AA, HEINZLE G, et al. Accuracy of MDCT in the determination of supraaortic artery stenosis using DSA as the reference standard. Eur J Radiol.2011; 80(3):351-355.
[17] YOSHINORI T, AKIHIKO K, YUTAKA K, et al. Clinical Experience of Dual-phase Cone Beam Computed Tomography during Hepatic Arteriography to Apply 3D-DSA. Japan J Radiol Technol. 2016;72(11):1091-1097.
[18] BLAGOJEVIC J, PIEMONTE G, BENELLI L, et al. Assessment, definition, and classification of lower limb ulcers in systemic sclerosis: a challenge for the rheumatologist. J Rheumatol.2016;43(3):592-598.
[19] 沈海刚,何俊玲,李鸣. DSA造影确定下肢动脉硬化闭塞性肢体坏疽截肢平面的评估[J].介入放射学杂志,2005,14(1):71-72.
[20] ABD-ELGAWAD EA, IBRAHEEM MA, SAMY LA, et al. Assessment of the distal runoff in patients with long standing diabetes mellitus and lower limb ischemia: MDCTA versus DSA. Egypt J Radiol Nucl Med. 2013;44(2):231-236.
[21] MCCOLLOUGH CH, MILLER WP, LYSEL MS, et al. Densitometric assessment of regional left ventricular systolic function during graded ischemia in the dog by use of dual-energy digital subtraction ventriculography. Am Heart J. 1993;125(6):1667-1675.
[22] 秦治刚,于伟东,杨小玉,等. DSA引导下山羊缺血性脊髓损伤模型的建立及形态学实验研究[J].中国实验诊断学,2011,15(2): 319-321.
[23] LIU B, JIANG Q, LIU W, et al. A vessel segmentation method for serialized cerebralvascular DSA images based on spatial feature point set of rotating coordinate system. Comput Methods Programs Biomed. 2018;161:55-72.
[24] 焦河,王凤英. DSA技术在兔髂总动脉造影中的应用[J].西部医学, 2009,21(4):550-552.
[25] 袁占奎,齐长明.舒泰和速眠新Ⅱ对犬呼吸和心血管系统影响的比较[J].中国兽医杂志,2009,45(1):62-64.
[26] 张世龙,翟仁友,戴定可,等. 高浓度造影剂和高速摄影在动物实验中获取高质量影像的应用[J].中国实验方剂学杂志,2007, 13(8):54-57.
[27] 李清军,魏勤,赵红朴,等.DSA检查中对比剂的流速及其选择[J]. 医学影像学杂志,2002,12(5):3-5.