Application and characteristics of decellularization technology in tissue engineered trachea

doi:10.3969/j.issn.2095-4344.2773

Abstract

Abstract:

BACKGROUND: Repair of long tracheal injury has always been a major challenge in tracheal therapy. Tissue engineering tracheal decellularization technology can provide an effective treatment approach for long tracheal injury repair.

OBJECTIVE: To review the advantages and disadvantages of different acellular techniques in tissue engineered trachea and their application in tracheal replacement therapy.

METHODS: Using the terms “tissue engineered trachea, decellularization, tracheal reconstruction, tracheal replacement therapy” as key words, CNKI, Wanfang and PubMed database from January 2006 to November 2019 were analyzed and summarized.

RESULTS AND CONCLUSION: Long tracheal injury is mainly caused by tumor, trauma, stenosis, and infection. It requires tracheal replacement for airway reconstruction therapy, and the decellularized tracheal matrix prepared by acellular technology in tissue engineering research is an effective way for its treatment. Decellularization technology removes the immunogenicity of tissues or organs through physical, chemical and biological enzymes, and effectively retains the extracellular matrix of tissues and organs, providing a supportive environment for remodeling functional trachea. After surgery, patients do not need to rely on immunosuppressants. At present, there are cases of decellularization technology used in clinical tracheal transplantation, but patients have more complications after surgery, and its functions need to be further improved. With the development of decellularization technology, acellular methods have become more mature, sophisticated and diversified, providing directions and approaches for future clinical tracheal treatment.

Key words: tissue engineered trachea, decellularization, tracheal replacement therapy, tracheal reconstruction, extracellular matrix, detergent combined with enzyme method, vacuum-assisted decellularization, sodium dodecyl sulfate

CLC Number:

Wang Zhihao, Wu Cong, Wu Sihua, Shi Hongcan. Application and characteristics of decellularization technology in tissue engineered trachea[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(35): 5695-5700.

Figures/Tables 2

References

[1] ETIENNE H, FABRE D, CARO AG, et al. Tracheal replacement. Eur Respir J. 2018;51:17022112.

[2] CROWLEY C, BIRCHALL M, SEIFALIAN AM. Trachea transplantation: from laboratory to patient. Tissue Eng Regen M. 2015;9(4):357-367.

[3] FISHMAN J M, WILES K, LOWDELL MW, et al. Airway tissue engineering: an update. Expert Opin Biol Th. 2014;14(10): 1477-1491.

[4] 杨文龙,张思泉,张国中,等. 京尼平交联脱细胞气管支架种植自体骨髓间充质干细胞的体内原位移植研究[J].国际生物医学工程杂志, 2019,42(5):375-382.

[5] BOGAN SL, TEOH GZ, BIRCHALL MA. Tissue engineered airways: a prospects article. J Cell Biochem. 2016;117(7):1497-1505.

[6] GHORBANI F, MORADI L, SHADMEHR MB, et al. In-vivo characterization of a 3D hybrid scaffold based on PCL/decellularized aorta for tracheal tissue engineering. J Cell Biochem. 2017;81:74-83.

[7] BATIOGLU-KARAALTIN A, OVALI E, KARAALTIN MV, et al. Decellularization of trachea with combined techniques for tissue-engineered trachea transplantation. Clin Exp Otorhinolar. 2019; 12(1):86-94.

[8] ELLIOTT MJ, DE COPPI P, SPEGGIORIN S, et al. Stem-cell-based, tissue engineered tracheal replacement in a child: a 2-year follow-up study. Lancet. 2012;380(9846):994-1000.

[9] 蒋媛,徐艳飞,张思泉,等.两种脱细胞方法制备组织工程气管基质的比较[J].中国组织工程研究,2016,20(51):7642-7647.

[10] GILPIN A, YANG Y. Decellularization strategies for regenerative medicine: from processing techniques to applications. Biomed Res Int. 2017;2017:9831534.

[11] GOKTAS S, MATUSKA AM, PIERRE N, et al. Decellularization method influences early remodeling of an allogenic tissue scaffold. J Biomed Mater Res A. 2014;102(1):8-16.

[12] PETROSYAN A, DA SACCO S, TRIPURANENI N, et al. A step towards clinical application of acellular matrix: A clue from macrophage polarization. Matrix Biol. 2017;57-58(SI):334-346.

[13] FAULK DM, JOHNSON SA, ZHANG L, et al. Role of the extracellular matrix in whole organ engineering. J Cell Physiol. 2014;229(8): 984-989.

[14] CRAPO PM, GILBERT TW, BADYLAK SF. An overview of tissue and whole organ decellularization processes. Biomaterials. 2011; 32(12): 3233-3243.

[15] 范利梅,夏荣,窦晓晨.不同脱细胞方式下所得胞外基质存在的差异[J]. 中国组织工程研究,2014,18(30):4863-4867.

[16] FUNAMOTO S, NAM K, KIMURA T, et al. The use of high-hydrostatic pressure treatment to decellularize blood vessels. Biomaterials. 2010; 31(13):3590-3595.

[17] FLYNN LE. The use of decellularized adipose tissue to provide an inductive microenvironment for the adipogenic differentiation of human adipose-derived stem cells. Biomaterials. 2010;31(17): 4715-4724.

[18] XING Q, YATES K, TAHTINEN M, et al. Decellularization of fibroblast cell sheets for natural extracellular matrix scaffold preparation. Tissue Eng Part C Methods. 2015;21(1):77-87.

[19] Den HONDT M, VANAUDENAERDE BM, MAUGHAN EF, et al. An optimized non-destructive protocol for testing mechanical properties in decellularized rabbit trachea. Acta Biomater. 2017; 60:291-301.

[20] LANGE P, SHAH H, BIRCHALL M, et al. Characterization of a biologically derived rabbit tracheal scaffold. J Biomed Mater Res B. 2017;105(7):2126-2135.

[21] HUNG S, SU C, LIN S, et al. Preliminary experiences in trachea scaffold tissue engineering with segmental organ decellularization. Laryngoscope. 2016;126(11):2520-2527.

[22] SYED O, WALTERS N J, DAY RM, et al. Evaluation of decellularization protocols for production of tubular small intestine submucosa scaffolds for use in oesophageal tissue engineering. Acta Biomaterialia. 2014; 10(12):5043-5054.

[23] SUN F, PAN S, SHI H, et al. Structural integrity, immunogenicity and biomechanical evaluation of rabbit decelluarized tracheal matrix. J Biomed Mater Res A. 2015;103(4):1509-1519.

[24] CHEN L, HE S, ZHUANG J, et al. A novel method to acquire decellularized tracheal matrix from neonatal rabbit. J Biomater Tiss Eng. 2016;6(5):400-407.

[25] ZHANG F, WANG Z, ZHENG C, et al. Biocompatibility and cellular compatibility of decellularized tracheal matrix derived from rabbits. Int J Artif Organs. 2019;42(9):500-507.

[26] PARTINGTON L, MORDAN N J, MASON C, et al. Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds. Acta Biomater. 2013; 9(2):5251-5261.

[27] ZANG M, ZHANG Q, CHANG EI, et al. Decellularized tracheal matrix scaffold for tissue engineering. Plast Reconstr Surg. 2012; 130(3): 532-540.

[28] CEBOTARI S, TUDORACHE I, JAEKEL T, et al. Detergent decellularization of heart valves for tissue engineering: toxicological effects of residual detergents on human endothelial cells. Artif Organs. 2010;34(3):206-209.

[29] XU Y, LI D, YIN Z, et al. Tissue-engineered trachea regeneration using decellularized trachea matrix treated with laser micropore technique. Acta Biomater. 2017;2017,58:113-121.

[30] OHNO M, FUCHIMOTO Y, HSU H, et al. Airway reconstruction using decellularized tracheal allografts in a porcine model. Pediatr Surg Int. 2017;33(10):1065-1071.

[31] Den HONDT M, VANAUDENAERDE BM, VERBEKEN EK, et al. Epithelial grafting of a decellularized whole-tracheal segment: an in vivo experimental model. Interact Cardiov Th. 2018;26(5):753-760.

[32] ZANG M, ZHANG Q, CHANG E I, et al. Decellularized tracheal matrix scaffold for tracheal tissue engineering: in vivo host response. Plast Reconstr Surg. 2013;132(4):549E-559E.

[33] BAIGUERA S, DEL GAUDIO C, KUEVDA E, et al. Dynamic decellularization and cross-linking of rat tracheal matrix. Biomaterials. 2014;35(24):6344-6350.

[34] MACCHIARINI P, JUNGEBLUTH P, GO T, et al. Clinical transplantation of a tissue-engineered airway. Lancet. 2008; 372(9655): 2023-2030.

[35] GONFIOTTI A, JAUS MO, BARALE D, et al. The first tissue-engineered airway transplantation: 5-year follow-up results. Lancet. 2014;383(9913):238-244.

[36] GIRALDO-GOMEZ DM, LEON-MANCILLA B, DEL PRADO-AUDELO ML, et al. Trypsin as enhancement in cyclical tracheal decellularization: morphological and biophysical characterization. Mat Sci Eng C Mater. 2016;59:930-937.

[37] BUTLER CR, HYNDS RE, CROWLEY C, et al. Vacuum-assisted decellularization: an accelerated protocol to generate tissue-engineered human tracheal scaffolds. Biomaterials. 2017; 124:95-105.

[38] LANGE P, GRECO K, PARTINGTON L, et al. Pilot study of a novel vacuum-assisted method for decellularization of tracheae for clinical tissue engineering applications. J Tissue Eng Regen M. 2017;11(3): 800-811.

[39] KITAHARA H, YAGI H, TAJIMA K, et al. Heterotopic transplantation of a decellularized and recellularized whole porcine heart. Interact Cardiovasc Thorac Surg. 2016;22(5):571-579.

[40] REMLINGER NT, CZAJKA CA, JUHAS ME, et al. Hydrated xenogeneic decellularized tracheal matrix as a scaffold for tracheal reconstruction. Biomaterials. 2010;31(13):3520-3526.

[41] DIMOU Z, MICHALOPOULOS E, KATSIMPOULAS M, et al. Evaluation of a decellularization protocol for the development of a decellularized tracheal scaffold. Anticancer Res. 2019;39(1): 145-150.

[42] PAN S, SUN F, SHI H, et al. Evaluation of an immune-privileged scaffold for in vivo implantation of tissue-engineered trachea. Biotechnol Bioproc E. 2014;19(5):925-934.

[43] KISELEVSKY MV, ANISIMOVA NY, LEBEDINSKAYA OV, et al. Optimization of a method for preparation and repopulation of the tracheal matrix for allogenic transplantation. Bull Exp Biol Med. 2011;151(1):107-113.

[44] KISILEVSKII MV, ANISIMOVA N, LEBEDINSKAIA OV, et al. Heterotopic transplantation of non-immunogenic trachea populated with recipient bone marrow stromal cells. Morfologiia. 2012; 141(1): 66-70.

[45] BAIGUERA S, JUNGEBLUTH P, BURNS A, et al. Tissue engineered human tracheas for in vivo implantation. Biomaterials. 2010;31(34): 8931-8938.

[46] SUN F, JIANG Y, XU Y, et al. Genipin cross-linked decellularized tracheal tubular matrix for tracheal tissue engineering applications. Sci Rep Uk. 2016;6:24429.

[47] CHEN Y, CHEN J, ZHANG Z, et al. Current advances in the development of natural meniscus scaffolds: innovative approaches to decellularization and recellularization. Cell Tissue Res. 2017;370(1): 41-52.

[48] HAMILTON NJ, KANANI M, ROEBUCK DJ, et al. Tissue-engineered tracheal replacement in a child: a 4-year follow-up study. Am J Transplant. 2015;15(10):2750-2757.

[49] TAN Q, LIU R, CHEN X, et al. Clinic application of tissue engineered bronchus for lung cancer treatment. J Thorac Dis. 2017;9(1):22-29.