Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (28): 4518-4525.doi: 10.3969/j.issn.2095-4344.2284
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Guo Enhui, Xu Zitong, Liang Yize, Zhou Liang, Lu Zhaoxiang, You Liang, Xia Yujun
Medical College, Qingdao University, Qingdao 266071, Shandong Province, China
Received:
2019-10-21
Revised:
2019-10-25
Accepted:
2019-11-25
Online:
2020-10-08
Published:
2020-09-01
Contact:
Xia Yujun, PhD, Professor, Doctoral supervisor, Medical College, Qingdao University, Qingdao 266071, Shandong Province, China
About author:
Guo Enhui, Medical College, Qingdao University, Qingdao 266071, Shandong Province, China
Supported by:
the Qingdao Livelihood Science and Technology Program, No. 15-9-2-76-nsh
CLC Number:
Guo Enhui, Xu Zitong, Liang Yize, Zhou Liang, Lu Zhaoxiang, You Liang, Xia Yujun. Properties of a novel photocrosslinked fish collagen peptide-hyaluronic acid hydrogel[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(28): 4518-4525.
2.2 水凝胶的溶胀性 图3中显示了不同组分水凝胶材料在37 ℃去离子水中的溶胀曲线。 由图可见,在溶胀初期水凝胶的吸水溶胀速度较快,后期逐渐变缓至溶胀平衡,不同配比的水凝胶都可在100 min内达到溶胀平衡。对比各组,当鱼胶原蛋白肽质量浓度为 30 g/L时,水凝胶达溶胀平衡时的溶胀率为1 582%;当鱼胶原蛋白肽质量浓度为50 g/L时,水凝胶达溶胀平衡时的溶胀率为610%;当鱼胶原蛋白肽质量浓度为100 g/L时,水凝胶达溶胀平衡时的溶胀率仅为225%,可看出随着水凝胶中鱼胶原蛋白肽含量的增高,达溶胀平衡时的最大溶胀率迅速缩小,各组比较差异有显著性意义(P < 0.05)。尽管各组均具有较快的溶胀速率,但因配比不同,溶胀速率大小也呈A组>B组>C组的趋势,各组比较差异有显著性意义(P < 0.05)。 "
组织学观察显示,各组切片周围细胞排列规则,炎症均未波及皮肤及肌肉组织,未见组织变形坏死或异常增生;术后1周时,各组水凝胶周围均有少量炎性细胞及成纤维细胞浸润,炎性细胞以淋巴细胞为主,可见少量巨噬细胞,极少见中性粒细胞;术后2周时,周边炎性细胞浸润较第1周增多,可见淋巴细胞、成纤维细胞、新生血管长入凝胶;术后4周时,水凝胶周边炎性细胞浸润开始减少,纤维母细胞增生并分泌胶原纤维,水凝胶周围可见明显包裹的纤维薄膜,与周围组织界限清楚;术后6周时,周边炎性细胞浸润减少,组织细胞进一步长入凝胶;术后8周时,周边炎性浸润尚未完全消失,但已较少,凝胶内部大部分长入组织,见图6。各组炎性细胞计数见图7所示,术后1周各组水凝胶周围均有少量炎性细胞浸润,至术后2周时周边炎性细胞浸润明显增多,随后各个时间点炎性细胞浸润逐渐减少,各个时间点炎性细胞浸润程度均为A组>B组>C组,组间比较差异有显著性意义(P < 0.05)。 "
2.3.3 水凝胶的体内降解性 大体观察可见随时间延长水凝胶的体积不断缩小,降解程度A组>B组>C组,但至术后8周时都有凝胶残留,水凝胶仍保持原形态,未发生崩解,见图5。 组织学观察显示,术后1周时,各组仅在凝胶周边有少许组织细胞长入凝胶,凝胶内部完整,无组织细胞生长现象,A组组织细胞生长数目较B组多,C组仅少量细胞长入凝胶边缘。术后2周切时,组织细胞呈条索状长入各组凝胶,A组已有较多组织细胞长入凝胶内部,主要为成纤维细胞,淋巴细胞,极少见中性粒细胞,可见新生血管;B组也有组织细胞长入凝胶的情况,较A组为少;C组仅有少量组织细胞长入水凝胶。术后4周时,A组有更多组织细胞长入,成纤维细胞分泌胶原,新生血管进一步增多;B组亦有更多组织细胞长入,较A组为少;C组增多,但明显少于A、B两组。术后6周时,各组组织细胞进一步增多,凝胶逐渐吸收。术后8周时,凝胶内部大部分长入组织,A组凝胶残留已较少,凝胶降解吸收处组织形态已类似周围真皮组织;B组有较多凝胶残留;C组有大量水凝胶残留,水凝胶仍基本保持原来形态,尚未崩解,见图6。 各组水凝胶不同时间点的降解情况见图8,可见第2周降解速率较第1周明显加快,随后降解速率开始逐渐减慢,至第8周观察结束时,A组降解率达54%,B组降解率达45%,C组降解率达36%。各个时间点降解率相比均为A组>B组>C组,组间比较差异有显著性意义(P < 0.05)。 "
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