Effects of total saponins of Achyranthes bidentata on cytokine profiles of synovial fluid in experimental rabbits with knee osteoarthritis

doi:10.3969/j.issn.2095-4344.1842

Abstract

Abstract:

BACKGROUND: Previous studies have shown that synovial fluid cytokines are important factors responsible for knee pain. Achyranthes bidentata can promote the repair of knee chondrocytes, relieve inflammation of the knee joint, and ease knee pain. Whether Achyranthes bidentata can regulate the level of cytokines in the synovial fluid of knee osteoarthritis is still unclear.
OBJECTIVE: To investigate the effect of total saponins of Achyranthes bidentata on cytokine profiles of synovial fluid in experimental rabbits with knee osteoarthritis.
METHODS: Eight New Zealand rabbits were randomized into experimental and control groups (n=4/group). A rabbit model of knee osteoarthritis was established. At 2 days after modeling, the total saponins of Achyranthes bidentata and distilled water were administered in the experimental and control group, respectively. Range of motion of the knee joint was measured after 2 weeks of administration. The synovial fluid of the knee joint was extracted from each rabbit. The levels of inflammatory cytokines and chemokines were measured by Luminex. The levels of matrix metalloproteinases 1, 2, 3 mRNA were measured by ELISA. The study protocol was approved by the Animal Ethics Committee of Guangzhou University of Chinese Medicine (approval No. 2018-31).
RESULTS AND CONCLUSION: The range of motion of the knee joint in the experimental group was significantly higher than that in the control group (P < 0.05). Compared with the control group, the expression levels of inflammatory factors interleukin 1α, interleukin 1β, interleukin 10, tumor necrosis factor α, chemokines CCL3, CXCL8, CXCL9, matrix metalloproteinases 1, 2, 3 decreased significantly after treatment (P < 0.05). These findings indicate that Achyranthes bidentata total saponins can alleviate knee dysfunction in knee osteoarthritis rabbits, and reduce the level of cytokines in the synovial fluid of the knee joint, thus reducing inflammation and protecting the cartilage.

Key words: osteoarthritis, Achyranthes bidentata, total saponins of Achyranthes bidentata, synovial fluid, matrix metalloproteinase, cytokine, chemokine

CLC Number:

Gao Kun, Zhang Yong, Chen Dayu, Yu Weiji, Sun Shufen, Wu Yihong, Lin Zhanpeng, Cao Yafei. Effects of total saponins of Achyranthes bidentata on cytokine profiles of synovial fluid in experimental rabbits with knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(33): 5317-5321.

Figures/Tables 2

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