Antagonistic effect of methylene blue on aniline-induced DNA damage of mouse bone marrow cells

doi:10.3969/j.issn.2095-4344.1836

Abstract

Abstract:

BACKGROUND: With the continuous application of single cell gel electrophoresis technology in DNA damage and repair, we explore target organs of aniline genotoxicity and kinetic effects of methylene blue, providing a new strategy for the treatment of aniline poisoning symptoms.
OBJECTIVE: To investigate the antagonistic effect of methylene blue on aniline-induced DNA damage of bone marrow cells in mice.
METHODS: Kunming mice were intragastrically administered with aniline at 5, 50, 100, and 200 mg/kg (aniline-administered groups). Corresponding methylene blue treatment groups, negative control group, and blank control group were set. Ten mice in each group were exposed to poison for 6 days. The mouse bone marrow cells were taken for single cell gel electrophoresis to observe the DNA damage and repair of bone marrow cells.
RESULTS AND CONCLUSION: (1) Compared with the negative control group and the blank control group, the DNA tail length and tail moment of the bone marrow cells in 100 and 200 mg/kg aniline-administered groups increased significantly (P < 0.01). The tail length and tail moment of the 5 and 50 mg/kg methylene blue treatment groups were slightly lower than those of the corresponding aniline-administered groups (P > 0.05), while the tail length and tail moment of the 100 and 200 mg/kg aniline-administered group showed a significant increase as compared with the corresponding methylene blue treatment groups (P < 0.01). Overall findings indicate that DNA damage in mouse bone marrow cells gradually aggravates with the increase of aniline concentration, and methylene blue has a certain repair effect on aniline-induced DNA damage in mouse bone marrow cells.

Key words: DNA damage, DNA repair, genotoxicity, antagonism, comet assay, dose relationship, methylene blue, aniline, Kunming mice, bone marrow

CLC Number:

Jiang Chao, Hu Yulong. Antagonistic effect of methylene blue on aniline-induced DNA damage of mouse bone marrow cells[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(33): 5312-5316.

Figures/Tables 2

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